##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AvaI AvrII
BbeI BbrPI BcgI BfrI BglI
BlnI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BstBI BstUI Bsu36I
ClaI CpoI Eco47III Eco81I EcoRI
FauI FseI Hin1I HpaI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1264 GTATAC GT^[AC][GT]AC
AflIII 1000 ACGTGT A^C[GA][CT]GT
AhdI 932 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2473 GGGCCC GGGCC^C
AspEI 932 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 714 TGCGCA TGC^GCA
BalI 517 TGGCCA TGG^CCA
BamHI 623 GGATCC G^GATCC
BclI 843 TGATCA T^GATCA
BglII 531 AGATCT A^GATCT
BsaAI 999 TACGTG [CT]AC^GT[GA]
BsgI 1714 GTGCAG GTGCAG(16/14)
BsiEI 140 CGGCCG CG[GA][CT]^CG
BsmI 9 GCATTC GAATGC(1/-1)
BspHI 974 TCATGA T^CATGA
BssSI 1283 CTCGTG CACGAG(-5/-1)
Bst1107I 1264 GTATAC GTA^TAC
BstEII 1326 GGTCACC G^GT[ACGT]ACC
BstPI 1326 GGTCACC G^GT[ACGT]ACC
BstZ17I 1264 GTATAC GTA^TAC
DraI 2908 TTTAAA TTT^AAA
DraIII 1280 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1030 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 140 CGGCCG C^GGCCG
Eam1105I 932 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 140 CGGCCG C^GGCCG
Eco52I 140 CGGCCG C^GGCCG
EcoO65I 1326 GGTCACC G^GT[ACGT]ACC
EcoT22I 125 ATGCAT ATGCA^T
FbaI 843 TGATCA T^GATCA
FspI 714 TGCGCA TGC^GCA
HaeII 1666 AGCGCC [GA]GCGC^[CT]
HgaI 1086 GACGC GACGC(5/10)
HindIII 2884 AAGCTT A^AGCTT
MfeI 27 CAATTG C^AATTG
MluNI 517 TGGCCA TGG^CCA
MscI 517 TGGCCA TGG^CCA
MunI 27 CAATTG C^AATTG
NcoI 224 CCATGG C^CATGG
NheI 1802 GCTAGC G^CTAGC
NsiI 125 ATGCAT ATGCA^T
NspI 1932 GCATGT [GA]CATG^[CT]
PvuII 1047 CAGCTG CAG^CTG
RcaI 974 TCATGA T^CATGA
SexAI 912 ACCTGGT A^CC[AT]GGT
TfiI 852 GAATC G^A[AT]TC
XcmI 2568 CCAGGAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 609 GCGG CCGC(-3/-1)
708 GCGG
AlwNI 781 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
2374 CAGACACTG
AspI 316 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1815 GACCAAGTC
BciVI 441 GGATAC GTATCC(6/5)
1458 GGATAC
BcnI 2546 CCCGG CC^[GC]GG
2844 CCCGG
BsrFI 142 GCCGGT [GA]^CCGG[CT]
670 GCCGGT
BstAPI 1943 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2079 GCATCACCTGC
BstXI 1349 CCATCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1995 CCAAAACAGTGG
CfoI 715 GCGC GCG^C
1667 GCGC
Cfr10I 142 GCCGGT [GA]^CCGG[CT]
670 GCCGGT
DsaI 224 CCATGG C^C[GA][CT]GG
1499 CCGTGG
EcoNI 1680 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2049 CCTCAGCCAGG
EcoRV 195 GATATC GAT^ATC
2686 GATATC
HhaI 715 GCGC GCG^C
1667 GCGC
HinP1I 715 GCGC G^CGC
1667 GCGC
HincII 308 GTCAAC GT[CT]^[GA]AC
1189 GTCAAC
HindII 308 GTCAAC GT[CT]^[GA]AC
1189 GTCAAC
MspA1I 706 CAGCGG C[AC]G^C[GT]G
1047 CAGCTG
NciI 2546 CCCGG CC^[GC]GG
2844 CCCGG
PflFI 316 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1815 GACCAAGTC
PshAI 178 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2543 GACCCCGGTC
SapI 1244 GAAGAGC GCTCTTC(1/4)
2535 GAAGAGC
TaqI 245 TCGA T^CGA
1217 TCGA
Tth111I 316 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1815 GACCAAGTC
TthHB8I 245 TCGA T^CGA
1217 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BanI BanII BbsI BbvCI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII