##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BcgI BciVI BclI BglI BlnI
BlpI Bpu1102I BsaHI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI Hin1I KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 35 CGCG CG^CG
AflII 3663 CTTAAG C^TTAAG
Aor51HI 322 AGCGCT AGC^GCT
ApaI 1774 GGGCCC GGGCC^C
AseI 3993 ATTAAT AT^TAAT
AsnI 3993 ATTAAT AT^TAAT
AvaII 2000 GGTCC G^G[AT]CC
BbrPI 553 CACGTG CAC^GTG
BbsI 3421 GAAGAC GAAGAC(2/6)
BbvCI 1449 GCTGAGG CCTCAGC(-5/-2)
BcnI 1320 CCGGG CC^[GC]GG
BfrI 3663 CTTAAG C^TTAAG
BglII 2710 AGATCT A^GATCT
BpuAI 3421 GAAGAC GAAGAC(2/6)
BsaAI 553 CACGTG [CT]AC^GT[GA]
BsaBI 1195 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 781 GAGACC GGTCTC(1/5)
BsaWI 437 ACCGGA [AT]^CCGG[AT]
BspMI 2306 ACCTGC ACCTGC(4/8)
BstBI 3778 TTCGAA TT^CGAA
BstEII 2199 GGTGACC G^GT[ACGT]ACC
BstPI 2199 GGTGACC G^GT[ACGT]ACC
BstUI 35 CGCG CG^CG
DraII 1773 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 707 CGGCCA [CT]^GGCC[GA]
Eco47III 322 AGCGCT AGC^GCT
EcoO109I 1773 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2199 GGTGACC G^GT[ACGT]ACC
EcoRI 831 GAATTC G^AATTC
EcoT14I 1817 CCTTGG C^C[AT][AT]GG
HgaI 33 GACGC GACGC(5/10)
HpaI 2639 GTTAAC GTT^AAC
MamI 1195 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 28 CAGCTG C[AC]G^C[GT]G
MvnI 35 CGCG CG^CG
NciI 1320 CCGGG CC^[GC]GG
NdeI 2728 CATATG CA^TATG
NspV 3778 TTCGAA TT^CGAA
PflMI 1026 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 553 CACGTG CAC^GTG
PmlI 553 CACGTG CAC^GTG
PshBI 3993 ATTAAT AT^TAAT
PvuII 28 CAGCTG CAG^CTG
SfuI 3778 TTCGAA TT^CGAA
SpeI 268 ACTAGT A^CTAGT
SspI 1394 AATATT AAT^ATT
StuI 2763 AGGCCT AGG^CCT
StyI 1817 CCTTGG C^C[AT][AT]GG
Van91I 1026 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 2273 GTCTAC GT^[AC][GT]AC
2875 GTCTAC
BanI 2429 GGTGCC G^G[CT][GA]CC
3392 GGCACC
BseRI 3058 CTCCTC GAGGAG(10/8)
976 GAGGAG
BspLU11I 3439 ACATGT A^CATGT
3951 ACATGT
BsrDI 453 GCAATG GCAATG(2/0)
2820 GCAATG
BstAPI 294 GCAAGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1440 GCAGTACATGC
FauI 36 GCGGG CCCGC(4/6)
1723 GCGGG
HaeII 322 AGCGCT [GA]GCGC^[CT]
401 AGCGCC
HincII 1980 GTCAAC GT[CT]^[GA]AC
2639 GTTAAC
HindII 1980 GTCAAC GT[CT]^[GA]AC
2639 GTTAAC
HindIII 296 AAGCTT A^AGCTT
3682 AAGCTT
PacI 2810 TTAATTAA TTAAT^TAA
3990 TTAATTAA
SacI 1093 GAGCTC GAGCT^C
3512 GAGCTC
SapI 289 GAAGAGC GCTCTTC(1/4)
3514 GCTCTTC
ScaI 2681 AGTACT AGT^ACT
3587 AGTACT
SexAI 2203 ACCTGGT A^CC[AT]GGT
3499 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
BanII BbvI BfaI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI