################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I AocI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BcgI BciVI BclI BglI BlnI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI Hin1I KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 35 CGCG CG^CG AflII 3663 CTTAAG C^TTAAG Aor51HI 322 AGCGCT AGC^GCT ApaI 1774 GGGCCC GGGCC^C AseI 3993 ATTAAT AT^TAAT AsnI 3993 ATTAAT AT^TAAT AvaII 2000 GGTCC G^G[AT]CC BbrPI 553 CACGTG CAC^GTG BbsI 3421 GAAGAC GAAGAC(2/6) BbvCI 1449 GCTGAGG CCTCAGC(-5/-2) BcnI 1320 CCGGG CC^[GC]GG BfrI 3663 CTTAAG C^TTAAG BglII 2710 AGATCT A^GATCT BpuAI 3421 GAAGAC GAAGAC(2/6) BsaAI 553 CACGTG [CT]AC^GT[GA] BsaBI 1195 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 781 GAGACC GGTCTC(1/5) BsaWI 437 ACCGGA [AT]^CCGG[AT] BspMI 2306 ACCTGC ACCTGC(4/8) BstBI 3778 TTCGAA TT^CGAA BstEII 2199 GGTGACC G^GT[ACGT]ACC BstPI 2199 GGTGACC G^GT[ACGT]ACC BstUI 35 CGCG CG^CG DraII 1773 AGGGCCC [GA]G^G[ACGT]CC[CT] EaeI 707 CGGCCA [CT]^GGCC[GA] Eco47III 322 AGCGCT AGC^GCT EcoO109I 1773 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 2199 GGTGACC G^GT[ACGT]ACC EcoRI 831 GAATTC G^AATTC EcoT14I 1817 CCTTGG C^C[AT][AT]GG HgaI 33 GACGC GACGC(5/10) HpaI 2639 GTTAAC GTT^AAC MamI 1195 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 28 CAGCTG C[AC]G^C[GT]G MvnI 35 CGCG CG^CG NciI 1320 CCGGG CC^[GC]GG NdeI 2728 CATATG CA^TATG NspV 3778 TTCGAA TT^CGAA PflMI 1026 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 553 CACGTG CAC^GTG PmlI 553 CACGTG CAC^GTG PshBI 3993 ATTAAT AT^TAAT PvuII 28 CAGCTG CAG^CTG SfuI 3778 TTCGAA TT^CGAA SpeI 268 ACTAGT A^CTAGT SspI 1394 AATATT AAT^ATT StuI 2763 AGGCCT AGG^CCT StyI 1817 CCTTGG C^C[AT][AT]GG Van91I 1026 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 2273 GTCTAC GT^[AC][GT]AC 2875 GTCTAC BanI 2429 GGTGCC G^G[CT][GA]CC 3392 GGCACC BseRI 3058 CTCCTC GAGGAG(10/8) 976 GAGGAG BspLU11I 3439 ACATGT A^CATGT 3951 ACATGT BsrDI 453 GCAATG GCAATG(2/0) 2820 GCAATG BstAPI 294 GCAAGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1440 GCAGTACATGC FauI 36 GCGGG CCCGC(4/6) 1723 GCGGG HaeII 322 AGCGCT [GA]GCGC^[CT] 401 AGCGCC HincII 1980 GTCAAC GT[CT]^[GA]AC 2639 GTTAAC HindII 1980 GTCAAC GT[CT]^[GA]AC 2639 GTTAAC HindIII 296 AAGCTT A^AGCTT 3682 AAGCTT PacI 2810 TTAATTAA TTAAT^TAA 3990 TTAATTAA SacI 1093 GAGCTC GAGCT^C 3512 GAGCTC SapI 289 GAAGAGC GCTCTTC(1/4) 3514 GCTCTTC ScaI 2681 AGTACT AGT^ACT 3587 AGTACT SexAI 2203 ACCTGGT A^CC[AT]GGT 3499 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI BanII BbvI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI