##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BglI BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsoBI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
KasI KpnI KspI MamI MfeI
MluI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacII SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 5 CGCG CG^CG
AccIII 672 TCCGGA T^CCGGA
AcsI 308 GAATTT [GA]^AATT[CT]
AflIII 3391 ACGTGT A^C[GA][CT]GT
ApoI 308 GAATTT [GA]^AATT[CT]
Asp700I 3881 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1003 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2950 GAAGAC GAAGAC(2/6)
BpuAI 2950 GAAGAC GAAGAC(2/6)
BsaWI 672 TCCGGA [AT]^CCGG[AT]
BseAI 672 TCCGGA T^CCGGA
Bsp1407I 4524 TGTACA T^GTACA
BspEI 672 TCCGGA T^CCGGA
BsrGI 4524 TGTACA T^GTACA
BstEII 755 GGTCACC G^GT[ACGT]ACC
BstPI 755 GGTCACC G^GT[ACGT]ACC
BstUI 5 CGCG CG^CG
CpoI 1460 CGGTCCG CG^G[AT]CCG
DraIII 134 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3292 CCACGG C^C[GA][CT]GG
EcoO65I 755 GGTCACC G^GT[ACGT]ACC
HaeII 3528 AGCGCC [GA]GCGC^[CT]
HpaI 296 GTTAAC GTT^AAC
MroI 672 TCCGGA T^CCGGA
MvnI 5 CGCG CG^CG
PflFI 1003 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4097 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 1460 CGGTCCG CG^G[AT]CCG
SbfI 3532 CCTGCAGG CCTGCA^GG
SphI 2032 GCATGC GCATG^C
Sse8387I 3532 CCTGCAGG CCTGCA^GG
SspBI 4524 TGTACA T^GTACA
Tth111I 1003 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4097 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2567 TCTAGA T^CTAGA
XmnI 3881 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 1101 GGGCCC GGGCC^C
1191 GGGCCC
BalI 288 TGGCCA TGG^CCA
4134 TGGCCA
BglII 1131 AGATCT A^GATCT
3767 AGATCT
BsmBI 1541 CGTCTC CGTCTC(1/5)
535 GAGACG
BspHI 907 TCATGA T^CATGA
3813 TCATGA
BsrBI 982 GAGCGG CCGCTC(-3/-3)
1304 GAGCGG
BsrDI 2871 CATTGC GCAATG(2/0)
1800 GCAATG
BstXI 2391 CCATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2468 CCAGCAGAGTGG
DraI 3982 TTTAAA TTT^AAA
4534 TTTAAA
HgaI 3 GACGC GACGC(5/10)
537 GACGC
MluNI 288 TGGCCA TGG^CCA
4134 TGGCCA
MscI 288 TGGCCA TGG^CCA
4134 TGGCCA
NaeI 250 GCCGGC GCC^GGC
2917 GCCGGC
NgoMIV 250 GCCGGC G^CCGGC
2917 GCCGGC
NspI 2032 GCATGC [GA]CATG^[CT]
4347 ACATGC
RcaI 907 TCATGA T^CATGA
3813 TCATGA
SalI 1052 GTCGAC G^TCGAC
1854 GTCGAC
ScaI 3411 AGTACT AGT^ACT
4237 AGTACT
SexAI 711 ACCTGGT A^CC[AT]GGT
3672 ACCAGGT
SspI 4071 AATATT AAT^ATT
4596 AATATT
XcmI 853 CCAAGATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
950 CCAAACAGGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AfaI AflII AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaII BanI BanII BbvCI BbvI
BclI BcnI BfaI BfrI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII