##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AccII       AgeI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AspI        AviII       BalI        
BamHI       BbeI        BbvCI       BcgI        BsaBI       
BseRI       BsiEI       BsiWI       BsrBI       BsrFI       
BssHII      BssSI       BstBI       BstUI       Bsu36I      
Cfr10I      CpoI        DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HgaI        KasI        KspI        MamI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmeI        PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    Tth111I     Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII        3299  GACGTC               GACGT^C
AccIII       1910  TCCGGA               T^CCGGA
AclI         2367  AACGTT               AA^CGTT
AcyI         3299  GACGTC               G[GA]^CG[CT]C
ApaI          468  GGGCCC               GGGCC^C
Asp700I      1978  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        3723  CCTAGG               C^CTAGG
BbrPI         438  CACGTG               CAC^GTG
BbsI         3862  GAAGAC               GAAGAC(2/6)
BciVI         543  GGATAC               GTATCC(6/5)
BclI         3948  TGATCA               T^GATCA
BglI         4028  GCCTCTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3448  AGATCT               A^GATCT
BlnI         3723  CCTAGG               C^CTAGG
BpuAI        3862  GAAGAC               GAAGAC(2/6)
BsaAI         438  CACGTG               [CT]AC^GT[GA]
BsaHI        3299  GACGTC               G[GA]^CG[CT]C
BsaI         3513  GAGACC               GGTCTC(1/5)
BsaWI        1910  TCCGGA               [AT]^CCGG[AT]
BseAI        1910  TCCGGA               T^CCGGA
BsgI         3320  GTGCAG               GTGCAG(16/14)
BsmBI         860  GAGACG               CGTCTC(1/5)
BsmI         2511  GCATTC               GAATGC(1/-1)
BspDI        3880  ATCGAT               AT^CGAT
BspEI        1910  TCCGGA               T^CCGGA
BspHI        2081  TCATGA               T^CATGA
BsrDI        4372  GCAATG               GCAATG(2/0)
BstEII        705  GGTGACC              G^GT[ACGT]ACC
BstPI         705  GGTGACC              G^GT[ACGT]ACC
BstXI         437  CCACGTGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        3448  AGATCT               [GA]^GATC[CT]
ClaI         3880  ATCGAT               AT^CGAT
EcoO65I       705  GGTGACC              G^GT[ACGT]ACC
FauI          695  GCGGG                CCCGC(4/6)
FbaI         3948  TGATCA               T^GATCA
Hin1I        3299  GACGTC               G[GA]^CG[CT]C
HindIII      3389  AAGCTT               A^AGCTT
HpaI         3081  GTTAAC               GTT^AAC
MflI         3448  AGATCT               [GA]^GATC[CT]
MroI         1910  TCCGGA               T^CCGGA
NcoI          810  CCATGG               C^CATGG
NheI         2706  GCTAGC               G^CTAGC
PaeR7I       2097  CTCGAG               C^TCGAG
PmaCI         438  CACGTG               CAC^GTG
PmlI          438  CACGTG               CAC^GTG
PpuMI        1685  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         402  GACTTCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2367  AACGTT               AA^CGTT
PstI         1805  CTGCAG               CTGCA^G
RcaI         2081  TCATGA               T^CATGA
ScaI          801  AGTACT               AGT^ACT
StuI         4035  AGGCCT               AGG^CCT
SwaI         2883  ATTTAAAT             ATTT^AAAT
XcmI           29  CCAGTGGCGATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2097  CTCGAG               C^TCGAG
XhoII        3448  AGATCT               [GA]^GATC[CT]
XmnI         1978  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

Acc65I         78  GGTACC               G^GTACC
             1368  GGTACC              
AflIII       2086  ACATGT               A^C[GA][CT]GT
             2906  ACATGT              
AhdI          679  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1396  GACAGAAAGTC         
AlwI         1567  GGATC                GGATC(4/5)
             3829  GGATC               
AlwNI        1787  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3435  CAGGTTCTG           
AseI         1617  ATTAAT               AT^TAAT
             4502  ATTAAT              
AsnI         1617  ATTAAT               AT^TAAT
             4502  ATTAAT              
Asp718I        78  GGTACC               G^GTACC
             1368  GGTACC              
AspEI         679  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1396  GACAGAAAGTC         
AvaI          327  CCCGAG               C^[CT]CG[GA]G
             2097  CTCGAG              
BlpI           58  GCTCAGC              GC^T[ACGT]AGC
             2117  GCTTAGC             
Bpu1102I       58  GCTCAGC              GC^T[ACGT]AGC
             2117  GCTTAGC             
BsoBI         327  CCCGAG               C^[CT]CG[GA]G
             2097  CTCGAG              
Bsp1407I     2472  TGTACA               T^GTACA
             2903  TGTACA              
BspLU11I     2086  ACATGT               A^CATGT
             2906  ACATGT              
BsrGI        2472  TGTACA               T^GTACA
             2903  TGTACA              
Bst1107I       16  GTATAC               GTA^TAC
             2411  GTATAC              
BstAPI       1807  GCAGGGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3975  GCAGAAAGTGC         
BstZ17I        16  GTATAC               GTA^TAC
             2411  GTATAC              
CelII          58  GCTCAGC              GC^T[ACGT]AGC
             2117  GCTTAGC             
CfoI          496  GCGC                 GCG^C
             3306  GCGC                
DraIII        187  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4142  CACAATGTG           
DsaI          810  CCATGG               C^C[GA][CT]GG
             2104  CCGTGG              
Eam1105I      679  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1396  GACAGAAAGTC         
HhaI          496  GCGC                 GCG^C
             3306  GCGC                
HinP1I        496  GCGC                 G^CGC
             3306  GCGC                
KpnI           78  GGTACC               GGTAC^C
             1368  GGTACC              
PshBI        1617  ATTAAT               AT^TAAT
             4502  ATTAAT              
SacI          218  GAGCTC               GAGCT^C
              815  GAGCTC              
SapI         1073  GAAGAGC              GCTCTTC(1/4)
             4096  GCTCTTC             
SpeI         1286  ACTAGT               A^CTAGT
             2172  ACTAGT              
SspBI        2472  TGTACA               T^GTACA
             2903  TGTACA              
SspI         1601  AATATT               AAT^ATT
             2600  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AluI        ApoI        AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BfrI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XbaI