################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AccII AgeI Alw44I AocI Aor51HI ApaLI AscI AspI AviII BalI BamHI BbeI BbvCI BcgI BsaBI BseRI BsiEI BsiWI BsrBI BsrFI BssHII BssSI BstBI BstUI Bsu36I Cfr10I CpoI DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI KasI KspI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 3299 GACGTC GACGT^C AccIII 1910 TCCGGA T^CCGGA AclI 2367 AACGTT AA^CGTT AcyI 3299 GACGTC G[GA]^CG[CT]C ApaI 468 GGGCCC GGGCC^C Asp700I 1978 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 3723 CCTAGG C^CTAGG BbrPI 438 CACGTG CAC^GTG BbsI 3862 GAAGAC GAAGAC(2/6) BciVI 543 GGATAC GTATCC(6/5) BclI 3948 TGATCA T^GATCA BglI 4028 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3448 AGATCT A^GATCT BlnI 3723 CCTAGG C^CTAGG BpuAI 3862 GAAGAC GAAGAC(2/6) BsaAI 438 CACGTG [CT]AC^GT[GA] BsaHI 3299 GACGTC G[GA]^CG[CT]C BsaI 3513 GAGACC GGTCTC(1/5) BsaWI 1910 TCCGGA [AT]^CCGG[AT] BseAI 1910 TCCGGA T^CCGGA BsgI 3320 GTGCAG GTGCAG(16/14) BsmBI 860 GAGACG CGTCTC(1/5) BsmI 2511 GCATTC GAATGC(1/-1) BspDI 3880 ATCGAT AT^CGAT BspEI 1910 TCCGGA T^CCGGA BspHI 2081 TCATGA T^CATGA BsrDI 4372 GCAATG GCAATG(2/0) BstEII 705 GGTGACC G^GT[ACGT]ACC BstPI 705 GGTGACC G^GT[ACGT]ACC BstXI 437 CCACGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 3448 AGATCT [GA]^GATC[CT] ClaI 3880 ATCGAT AT^CGAT EcoO65I 705 GGTGACC G^GT[ACGT]ACC FauI 695 GCGGG CCCGC(4/6) FbaI 3948 TGATCA T^GATCA Hin1I 3299 GACGTC G[GA]^CG[CT]C HindIII 3389 AAGCTT A^AGCTT HpaI 3081 GTTAAC GTT^AAC MflI 3448 AGATCT [GA]^GATC[CT] MroI 1910 TCCGGA T^CCGGA NcoI 810 CCATGG C^CATGG NheI 2706 GCTAGC G^CTAGC PaeR7I 2097 CTCGAG C^TCGAG PmaCI 438 CACGTG CAC^GTG PmlI 438 CACGTG CAC^GTG PpuMI 1685 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 402 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2367 AACGTT AA^CGTT PstI 1805 CTGCAG CTGCA^G RcaI 2081 TCATGA T^CATGA ScaI 801 AGTACT AGT^ACT StuI 4035 AGGCCT AGG^CCT SwaI 2883 ATTTAAAT ATTT^AAAT XcmI 29 CCAGTGGCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2097 CTCGAG C^TCGAG XhoII 3448 AGATCT [GA]^GATC[CT] XmnI 1978 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 78 GGTACC G^GTACC 1368 GGTACC AflIII 2086 ACATGT A^C[GA][CT]GT 2906 ACATGT AhdI 679 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1396 GACAGAAAGTC AlwI 1567 GGATC GGATC(4/5) 3829 GGATC AlwNI 1787 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 3435 CAGGTTCTG AseI 1617 ATTAAT AT^TAAT 4502 ATTAAT AsnI 1617 ATTAAT AT^TAAT 4502 ATTAAT Asp718I 78 GGTACC G^GTACC 1368 GGTACC AspEI 679 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1396 GACAGAAAGTC AvaI 327 CCCGAG C^[CT]CG[GA]G 2097 CTCGAG BlpI 58 GCTCAGC GC^T[ACGT]AGC 2117 GCTTAGC Bpu1102I 58 GCTCAGC GC^T[ACGT]AGC 2117 GCTTAGC BsoBI 327 CCCGAG C^[CT]CG[GA]G 2097 CTCGAG Bsp1407I 2472 TGTACA T^GTACA 2903 TGTACA BspLU11I 2086 ACATGT A^CATGT 2906 ACATGT BsrGI 2472 TGTACA T^GTACA 2903 TGTACA Bst1107I 16 GTATAC GTA^TAC 2411 GTATAC BstAPI 1807 GCAGGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3975 GCAGAAAGTGC BstZ17I 16 GTATAC GTA^TAC 2411 GTATAC CelII 58 GCTCAGC GC^T[ACGT]AGC 2117 GCTTAGC CfoI 496 GCGC GCG^C 3306 GCGC DraIII 187 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 4142 CACAATGTG DsaI 810 CCATGG C^C[GA][CT]GG 2104 CCGTGG Eam1105I 679 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1396 GACAGAAAGTC HhaI 496 GCGC GCG^C 3306 GCGC HinP1I 496 GCGC G^CGC 3306 GCGC KpnI 78 GGTACC GGTAC^C 1368 GGTACC PshBI 1617 ATTAAT AT^TAAT 4502 ATTAAT SacI 218 GAGCTC GAGCT^C 815 GAGCTC SapI 1073 GAAGAGC GCTCTTC(1/4) 4096 GCTCTTC SpeI 1286 ACTAGT A^CTAGT 2172 ACTAGT SspBI 2472 TGTACA T^GTACA 2903 TGTACA SspI 1601 AATATT AAT^ATT 2600 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BfrI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI