##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccII AgeI Alw44I AocI Aor51HI
ApaLI AscI AspI AviII BalI
BamHI BbeI BbvCI BcgI BsaBI
BseRI BsiEI BsiWI BsrBI BsrFI
BssHII BssSI BstBI BstUI Bsu36I
Cfr10I CpoI DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI KasI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 3299 GACGTC GACGT^C
AccIII 1910 TCCGGA T^CCGGA
AclI 2367 AACGTT AA^CGTT
AcyI 3299 GACGTC G[GA]^CG[CT]C
ApaI 468 GGGCCC GGGCC^C
Asp700I 1978 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3723 CCTAGG C^CTAGG
BbrPI 438 CACGTG CAC^GTG
BbsI 3862 GAAGAC GAAGAC(2/6)
BciVI 543 GGATAC GTATCC(6/5)
BclI 3948 TGATCA T^GATCA
BglI 4028 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3448 AGATCT A^GATCT
BlnI 3723 CCTAGG C^CTAGG
BpuAI 3862 GAAGAC GAAGAC(2/6)
BsaAI 438 CACGTG [CT]AC^GT[GA]
BsaHI 3299 GACGTC G[GA]^CG[CT]C
BsaI 3513 GAGACC GGTCTC(1/5)
BsaWI 1910 TCCGGA [AT]^CCGG[AT]
BseAI 1910 TCCGGA T^CCGGA
BsgI 3320 GTGCAG GTGCAG(16/14)
BsmBI 860 GAGACG CGTCTC(1/5)
BsmI 2511 GCATTC GAATGC(1/-1)
BspDI 3880 ATCGAT AT^CGAT
BspEI 1910 TCCGGA T^CCGGA
BspHI 2081 TCATGA T^CATGA
BsrDI 4372 GCAATG GCAATG(2/0)
BstEII 705 GGTGACC G^GT[ACGT]ACC
BstPI 705 GGTGACC G^GT[ACGT]ACC
BstXI 437 CCACGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3448 AGATCT [GA]^GATC[CT]
ClaI 3880 ATCGAT AT^CGAT
EcoO65I 705 GGTGACC G^GT[ACGT]ACC
FauI 695 GCGGG CCCGC(4/6)
FbaI 3948 TGATCA T^GATCA
Hin1I 3299 GACGTC G[GA]^CG[CT]C
HindIII 3389 AAGCTT A^AGCTT
HpaI 3081 GTTAAC GTT^AAC
MflI 3448 AGATCT [GA]^GATC[CT]
MroI 1910 TCCGGA T^CCGGA
NcoI 810 CCATGG C^CATGG
NheI 2706 GCTAGC G^CTAGC
PaeR7I 2097 CTCGAG C^TCGAG
PmaCI 438 CACGTG CAC^GTG
PmlI 438 CACGTG CAC^GTG
PpuMI 1685 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 402 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2367 AACGTT AA^CGTT
PstI 1805 CTGCAG CTGCA^G
RcaI 2081 TCATGA T^CATGA
ScaI 801 AGTACT AGT^ACT
StuI 4035 AGGCCT AGG^CCT
SwaI 2883 ATTTAAAT ATTT^AAAT
XcmI 29 CCAGTGGCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2097 CTCGAG C^TCGAG
XhoII 3448 AGATCT [GA]^GATC[CT]
XmnI 1978 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 78 GGTACC G^GTACC
1368 GGTACC
AflIII 2086 ACATGT A^C[GA][CT]GT
2906 ACATGT
AhdI 679 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1396 GACAGAAAGTC
AlwI 1567 GGATC GGATC(4/5)
3829 GGATC
AlwNI 1787 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
3435 CAGGTTCTG
AseI 1617 ATTAAT AT^TAAT
4502 ATTAAT
AsnI 1617 ATTAAT AT^TAAT
4502 ATTAAT
Asp718I 78 GGTACC G^GTACC
1368 GGTACC
AspEI 679 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1396 GACAGAAAGTC
AvaI 327 CCCGAG C^[CT]CG[GA]G
2097 CTCGAG
BlpI 58 GCTCAGC GC^T[ACGT]AGC
2117 GCTTAGC
Bpu1102I 58 GCTCAGC GC^T[ACGT]AGC
2117 GCTTAGC
BsoBI 327 CCCGAG C^[CT]CG[GA]G
2097 CTCGAG
Bsp1407I 2472 TGTACA T^GTACA
2903 TGTACA
BspLU11I 2086 ACATGT A^CATGT
2906 ACATGT
BsrGI 2472 TGTACA T^GTACA
2903 TGTACA
Bst1107I 16 GTATAC GTA^TAC
2411 GTATAC
BstAPI 1807 GCAGGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3975 GCAGAAAGTGC
BstZ17I 16 GTATAC GTA^TAC
2411 GTATAC
CelII 58 GCTCAGC GC^T[ACGT]AGC
2117 GCTTAGC
CfoI 496 GCGC GCG^C
3306 GCGC
DraIII 187 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4142 CACAATGTG
DsaI 810 CCATGG C^C[GA][CT]GG
2104 CCGTGG
Eam1105I 679 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1396 GACAGAAAGTC
HhaI 496 GCGC GCG^C
3306 GCGC
HinP1I 496 GCGC G^CGC
3306 GCGC
KpnI 78 GGTACC GGTAC^C
1368 GGTACC
PshBI 1617 ATTAAT AT^TAAT
4502 ATTAAT
SacI 218 GAGCTC GAGCT^C
815 GAGCTC
SapI 1073 GAAGAGC GCTCTTC(1/4)
4096 GCTCTTC
SpeI 1286 ACTAGT A^CTAGT
2172 ACTAGT
SspBI 2472 TGTACA T^GTACA
2903 TGTACA
SspI 1601 AATATT AAT^ATT
2600 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BfrI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI