##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII AvrII BanI BbeI
BbvCI BcgI BglI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BstAPI
BstBI BstEII BstPI CelII ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FauI FseI
FspI KasI KpnI KspI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PacI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SmaI
Sse8387I SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 3 CGCG CG^CG
AcyI 115 GGCGTC G[GA]^CG[CT]C
AflII 1734 CTTAAG C^TTAAG
AseI 4099 ATTAAT AT^TAAT
AsnI 4099 ATTAAT AT^TAAT
AspI 2184 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 266 GGACC G^G[AT]CC
BbrPI 1360 CACGTG CAC^GTG
BcnI 1483 CCGGG CC^[GC]GG
BfrI 1734 CTTAAG C^TTAAG
BsaBI 777 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 115 GGCGTC G[GA]^CG[CT]C
BsaI 3110 GAGACC GGTCTC(1/5)
BseRI 2599 CTCCTC GAGGAG(10/8)
Bst1107I 2865 GTATAC GTA^TAC
BstUI 3 CGCG CG^CG
BstXI 4980 CCAATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2865 GTATAC GTA^TAC
DraII 3899 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 4107 GACCCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 22 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3899 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1390 GAATTC G^AATTC
HaeII 109 AGCGCC [GA]GCGC^[CT]
Hin1I 115 GGCGTC G[GA]^CG[CT]C
HpaI 3484 GTTAAC GTT^AAC
MamI 777 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3 CGCG CG^CG
NciI 1483 CCGGG CC^[GC]GG
NcoI 2622 CCATGG C^CATGG
PaeR7I 75 CTCGAG C^TCGAG
PflFI 2184 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2803 CCACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1360 CACGTG CAC^GTG
PmlI 1360 CACGTG CAC^GTG
PshBI 4099 ATTAAT AT^TAAT
PvuII 3095 CAGCTG CAG^CTG
SapI 929 GAAGAGC GCTCTTC(1/4)
ScaI 3404 AGTACT AGT^ACT
SgrAI 111 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 1941 ACTAGT A^CTAGT
Tth111I 2184 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2803 CCACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 75 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 1056 GTGCAC G^TGCAC
4370 GTGCAC
ApaLI 1056 GTGCAC G^TGCAC
4370 GTGCAC
BalI 438 TGGCCA TGG^CCA
2378 TGGCCA
BamHI 513 GGATCC G^GATCC
1558 GGATCC
BanII 17 GGGCTC G[GA]GC[CT]^C
3367 GGGCTC
BciVI 2400 GGATAC GTATCC(6/5)
3212 GGATAC
BglII 2346 AGATCT A^GATCT
4741 AGATCT
BsaWI 978 ACCGGA [AT]^CCGG[AT]
1062 ACCGGA
BsgI 2982 CTGCAC GTGCAG(16/14)
998 GTGCAG
BspMI 417 ACCTGC ACCTGC(4/8)
3756 ACCTGC
BsrFI 112 GCCGGC [GA]^CCGG[CT]
1084 GCCGGC
BssSI 1635 CACGAG CACGAG(-5/-1)
85 CTCGTG
CfoI 110 GCGC GCG^C
2056 GCGC
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
1084 GCCGGC
DraIII 518 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2930 CACCATGTG
DsaI 658 CCACGG C^C[GA][CT]GG
2622 CCATGG
EcoT14I 628 CCAAGG C^C[AT][AT]GG
2622 CCATGG
HgaI 57 GCGTC GACGC(5/10)
116 GCGTC
HhaI 110 GCGC GCG^C
2056 GCGC
HinP1I 110 GCGC G^CGC
2056 GCGC
HincII 3484 GTTAAC GT[CT]^[GA]AC
5209 GTTGAC
HindII 3484 GTTAAC GT[CT]^[GA]AC
5209 GTTGAC
HindIII 794 AAGCTT A^AGCTT
4538 AAGCTT
MluNI 438 TGGCCA TGG^CCA
2378 TGGCCA
MscI 438 TGGCCA TGG^CCA
2378 TGGCCA
MspA1I 1079 CCGCTG C[AC]G^C[GT]G
3095 CAGCTG
NaeI 112 GCCGGC GCC^GGC
1084 GCCGGC
NdeI 3123 CATATG CA^TATG
5188 CATATG
NgoMIV 112 GCCGGC G^CCGGC
1084 GCCGGC
SnaBI 743 TACGTA TAC^GTA
3463 TACGTA
StyI 628 CCAAGG C^C[AT][AT]GG
2622 CCATGG
XbaI 872 TCTAGA T^CTAGA
4870 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI EaeI EarI Eco81I EcoRII
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspBI
SspI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI