##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AcsI AflII ApoI AscI
AvrII BfrI BlnI BsiWI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
MluI NdeI NotI NruI NsiI
NspV PacI PaeR7I PmeI PvuI
RsrII SalI SfiI SfuI SnaBI
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 900 GGTACC G^GTACC
AccI 3141 GTCTAC GT^[AC][GT]AC
AccIII 562 TCCGGA T^CCGGA
AclI 4021 AACGTT AA^CGTT
AgeI 906 ACCGGT A^CCGGT
Alw44I 1703 GTGCAC G^TGCAC
AocI 170 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2084 AGCGCT AGC^GCT
ApaLI 1703 GTGCAC G^TGCAC
AseI 3977 ATTAAT AT^TAAT
AsnI 3977 ATTAAT AT^TAAT
Asp718I 900 GGTACC G^GTACC
AspI 2233 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2 GGCGCC GGCGC^C
BbrPI 3594 CACGTG CAC^GTG
BciVI 2194 GTATCC GTATCC(6/5)
BclI 2066 TGATCA T^GATCA
BglII 3680 AGATCT A^GATCT
BsaAI 3594 CACGTG [CT]AC^GT[GA]
BseAI 562 TCCGGA T^CCGGA
BspEI 562 TCCGGA T^CCGGA
BspHI 4068 TCATGA T^CATGA
BspLU11I 3130 ACATGT A^CATGT
BsrDI 1526 GCAATG GCAATG(2/0)
BssSI 1213 CACGAG CACGAG(-5/-1)
BstAPI 258 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 170 CCTCAGG CC^T[ACGT]AGG
DraIII 1034 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2084 AGCGCT AGC^GCT
Eco81I 170 CCTCAGG CC^T[ACGT]AGG
FbaI 2066 TGATCA T^GATCA
KasI 2 GGCGCC G^GCGCC
KpnI 900 GGTACC GGTAC^C
MfeI 3810 CAATTG C^AATTG
MroI 562 TCCGGA T^CCGGA
MunI 3810 CAATTG C^AATTG
NarI 2 GGCGCC GG^CGCC
NheI 2610 GCTAGC G^CTAGC
PflFI 2233 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 906 ACCGGT A^CCGGT
PmaCI 3594 CACGTG CAC^GTG
PmlI 3594 CACGTG CAC^GTG
PshBI 3977 ATTAAT AT^TAAT
Psp1406I 4021 AACGTT AA^CGTT
RcaI 4068 TCATGA T^CATGA
SbfI 888 CCTGCAGG CCTGCA^GG
ScaI 494 AGTACT AGT^ACT
SfoI 2 GGCGCC GGC^GCC
SgrAI 905 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1547 CCCGGG CCC^GGG
SpeI 12 ACTAGT A^CTAGT
SphI 870 GCATGC GCATG^C
Sse8387I 888 CCTGCAGG CCTGCA^GG
Tth111I 2233 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1824 TCTAGA T^CTAGA
XmaCI 1547 CCCGGG C^CCGGG
XmaI 1547 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 2 GGCGCC G[GA]^CG[CT]C
2776 GACGCC
AflIII 3130 ACATGT A^C[GA][CT]GT
4044 ACGTGT
AhdI 3288 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACTTCTGGTC
AspEI 3288 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACTTCTGGTC
AviII 17 TGCGCA TGC^GCA
1667 TGCGCA
BalI 112 TGGCCA TGG^CCA
609 TGGCCA
BamHI 543 GGATCC G^GATCC
884 GGATCC
BcgI 1764 CGAGCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2803 CGAGCGGTCTGC
BlpI 2734 GCTAAGC GC^T[ACGT]AGC
2847 GCTAAGC
Bpu1102I 2734 GCTAAGC GC^T[ACGT]AGC
2847 GCTAAGC
BsaBI 1328 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3239 GATTCTTATC
BsaHI 2 GGCGCC G[GA]^CG[CT]C
2776 GACGCC
BsaWI 562 TCCGGA [AT]^CCGG[AT]
906 ACCGGT
BsgI 184 CTGCAC GTGCAG(16/14)
2625 CTGCAC
BsiEI 523 CGGCCG CG[GA][CT]^CG
570 CGGCCG
BstXI 2313 CCAGACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3564 CCAGCTACTTGG
CelII 2734 GCTAAGC GC^T[ACGT]AGC
2847 GCTAAGC
EagI 523 CGGCCG C^GGCCG
570 CGGCCG
Eam1105I 3288 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACTTCTGGTC
EclXI 523 CGGCCG C^GGCCG
570 CGGCCG
Eco52I 523 CGGCCG C^GGCCG
570 CGGCCG
FspI 17 TGCGCA TGC^GCA
1667 TGCGCA
HaeII 2 GGCGCC [GA]GCGC^[CT]
2084 AGCGCT
Hin1I 2 GGCGCC G[GA]^CG[CT]C
2776 GACGCC
KspI 74 CCGCGG CCGC^GG
771 CCGCGG
MamI 1328 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3239 GATTCTTATC
MluNI 112 TGGCCA TGG^CCA
609 TGGCCA
MscI 112 TGGCCA TGG^CCA
609 TGGCCA
NaeI 66 GCCGGC GCC^GGC
572 GCCGGC
NgoMIV 66 GCCGGC G^CCGGC
572 GCCGGC
PflMI 2007 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3501 CCACAGAATGG
PshAI 3344 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4043 GACGTGTGTC
SacI 687 GAGCTC GAGCT^C
1765 GAGCTC
SacII 74 CCGCGG CCGC^GG
771 CCGCGG
SexAI 99 ACCAGGT A^CC[AT]GGT
3363 ACCAGGT
StuI 1577 AGGCCT AGG^CCT
2709 AGGCCT
TaqI 594 TCGA T^CGA
1481 TCGA
TthHB8I 594 TCGA T^CGA
1481 TCGA
Van91I 2007 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3501 CCACAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI