##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI FauI FseI FspI HapII
Hin1I HpaI HpaII KasI KpnI
KspI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AhdI 2744 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2958 GTGCAC G^TGCAC
ApaLI 2958 GTGCAC G^TGCAC
AspEI 2744 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3056 CTCGAG C^[CT]CG[GA]G
BalI 3244 TGGCCA TGG^CCA
BciVI 3110 GGATAC GTATCC(6/5)
BpmI 467 CTCCAG CTGGAG(16/14)
BsaBI 3287 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2458 GAGACC GGTCTC(1/5)
BsgI 1018 CTGCAC GTGCAG(16/14)
BsoBI 3056 CTCGAG C^[CT]CG[GA]G
BspHI 434 TCATGA T^CATGA
BsrBI 1395 CCGCTC CCGCTC(-3/-3)
DsaI 2411 CCATGG C^C[GA][CT]GG
EaeI 3244 TGGCCA [CT]^GGCC[GA]
Eam1105I 2744 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2484 CTCTTC CTCTTC(1/4)
EcoRV 632 GATATC GAT^ATC
EcoT22I 896 ATGCAT ATGCA^T
HaeII 649 GGCGCT [GA]GCGC^[CT]
HincII 869 GTCAAC GT[CT]^[GA]AC
HindII 869 GTCAAC GT[CT]^[GA]AC
Ksp632I 2484 CTCTTC CTCTTC(1/4)
MamI 3287 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3244 TGGCCA TGG^CCA
MscI 3244 TGGCCA TGG^CCA
NcoI 2411 CCATGG C^CATGG
NheI 524 GCTAGC G^CTAGC
NsiI 896 ATGCAT ATGCA^T
PaeR7I 3056 CTCGAG C^TCGAG
PleI 2349 GACTC GAGTC(4/5)
PstI 2445 CTGCAG CTGCA^G
RcaI 434 TCATGA T^CATGA
ScaI 2943 AGTACT AGT^ACT
SspI 2895 AATATT AAT^ATT
XcmI 3232 CCAGAGGATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3056 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflII 1023 CTTAAG C^TTAAG
1239 CTTAAG
AflIII 965 ACATGT A^C[GA][CT]GT
1364 ACATGT
Asp700I 2810 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3369 GAAGTGCTTC
BbvCI 3023 CCTCAGC CCTCAGC(-5/-2)
812 GCTGAGG
BcgI 3005 CGAGAAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3058 CGAGAAATGTGC
BfrI 1023 CTTAAG C^TTAAG
1239 CTTAAG
BglII 2480 AGATCT A^GATCT
3355 AGATCT
BspLU11I 965 ACATGT A^CATGT
1364 ACATGT
BsrDI 3070 CATTGC GCAATG(2/0)
609 GCAATG
BstAPI 1011 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3153 GCAAGTTCTGC
EcoNI 278 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2325 CCTAAAGAAGG
EcoT14I 2238 CCTTGG C^C[AT][AT]GG
2411 CCATGG
HgaI 1008 GACGC GACGC(5/10)
3049 GACGC
StyI 2238 CCTTGG C^C[AT][AT]GG
2411 CCATGG
XmnI 2810 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3369 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BclI BfaI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EcoO109I
EcoRII FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII