##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI AvrII BamHI BbeI BbrPI
BclI BlnI BlpI Bpu1102I BsaAI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspMI BssHII BstBI CelII
ClaI DraIII DrdI EagI EclXI
Eco47III Eco52I FbaI HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 11 CGCG CG^CG
AhdI 3140 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 981 CCTCAGG CC^T[ACGT]AGG
AspEI 3140 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2697 TGCGCA TGC^GCA
BalI 2346 TGGCCA TGG^CCA
BcgI 2978 CGAGGCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 751 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 3506 CGGTCG CG[GA][CT]^CG
BspLU11I 4088 ACATGT A^CATGT
BssSI 2828 CACGAG CACGAG(-5/-1)
Bst1107I 4300 GTATAC GTA^TAC
BstUI 11 CGCG CG^CG
BstXI 2210 CCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4300 GTATAC GTA^TAC
Bsu36I 981 CCTCAGG CC^T[ACGT]AGG
CpoI 326 CGGACCG CG^G[AT]CCG
Eam1105I 3140 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 981 CCTCAGG CC^T[ACGT]AGG
EcoNI 2794 CCTTTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 556 GATATC GAT^ATC
EcoT22I 2341 ATGCAT ATGCA^T
FseI 1309 GGCCGGCC GGCCGG^CC
FspI 2697 TGCGCA TGC^GCA
HaeII 3133 AGCGCC [GA]GCGC^[CT]
HincII 2134 GTTGAC GT[CT]^[GA]AC
HindII 2134 GTTGAC GT[CT]^[GA]AC
MluNI 2346 TGGCCA TGG^CCA
MscI 2346 TGGCCA TGG^CCA
MvnI 11 CGCG CG^CG
NdeI 2519 CATATG CA^TATG
NsiI 2341 ATGCAT ATGCA^T
PacI 3942 TTAATTAA TTAAT^TAA
RsrII 326 CGGACCG CG^G[AT]CCG
SacI 301 GAGCTC GAGCT^C
SmaI 3300 CCCGGG CCC^GGG
SspI 3908 AATATT AAT^ATT
StuI 2086 AGGCCT AGG^CCT
XmaCI 3300 CCCGGG C^CCGGG
XmaI 3300 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 748 GACGCC G[GA]^CG[CT]C
3740 GGCGTC
AflIII 2037 ACACGT A^C[GA][CT]GT
4088 ACATGT
AlwI 483 GATCC GGATC(4/5)
1348 GGATC
BanI 2221 GGTGCC G^G[CT][GA]CC
3211 GGTGCC
BbsI 745 GAAGAC GAAGAC(2/6)
3458 GTCTTC
BbvCI 1610 CCTCAGC CCTCAGC(-5/-2)
4095 GCTGAGG
BciVI 3480 GGATAC GTATCC(6/5)
2514 GTATCC
BglII 88 AGATCT A^GATCT
1200 AGATCT
BpuAI 745 GAAGAC GAAGAC(2/6)
3458 GTCTTC
BsaHI 748 GACGCC G[GA]^CG[CT]C
3740 GGCGTC
BsaWI 2277 TCCGGT [AT]^CCGG[AT]
2334 ACCGGA
Bsp1407I 731 TGTACA T^GTACA
4145 TGTACA
BsrGI 731 TGTACA T^GTACA
4145 TGTACA
BstAPI 789 GCACGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2749 GCAGAGGCTGC
BstEII 2246 GGTCACC G^GT[ACGT]ACC
3228 GGTTACC
BstPI 2246 GGTCACC G^GT[ACGT]ACC
3228 GGTTACC
CfoI 2698 GCGC GCG^C
3134 GCGC
EcoO65I 2246 GGTCACC G^GT[ACGT]ACC
3228 GGTTACC
EcoRI 141 GAATTC G^AATTC
437 GAATTC
HgaI 748 GACGC GACGC(5/10)
3741 GCGTC
HhaI 2698 GCGC GCG^C
3134 GCGC
Hin1I 748 GACGCC G[GA]^CG[CT]C
3740 GGCGTC
HinP1I 2698 GCGC G^CGC
3134 GCGC
NaeI 1310 GCCGGC GCC^GGC
1414 GCCGGC
NcoI 2019 CCATGG C^CATGG
2433 CCATGG
NgoMIV 1310 GCCGGC G^CCGGC
1414 GCCGGC
NspI 4088 ACATGT [GA]CATG^[CT]
4331 ACATGC
PpuMI 2804 GGGACCC [GA]G^G[AT]CC[CT]
2813 GGGACCC
PstI 2483 CTGCAG CTGCA^G
2747 CTGCAG
SspBI 731 TGTACA T^GTACA
4145 TGTACA
XbaI 3021 TCTAGA T^CTAGA
3553 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwNI ApaI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BanII BbvI BcnI BfaI BfrI
BmyI BpmI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PshBI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI