##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I ApaLI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BcgI BclI BglII
BlnI BsaHI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspMI BsrGI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI Eam1105I EcoRV EcoT22I
FbaI FseI FspI Hin1I HpaI
KasI KpnI KspI MluI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Aor51HI 3832 AGCGCT AGC^GCT
ApaI 3121 GGGCCC GGGCC^C
AscI 3823 GGCGCGCC GG^CGCGCC
BamHI 2825 GGATCC G^GATCC
BbrPI 308 CACGTG CAC^GTG
BciVI 4038 GTATCC GTATCC(6/5)
BsaAI 308 CACGTG [CT]AC^GT[GA]
BsiEI 38 CGGCCG CG[GA][CT]^CG
BsmI 3785 GCATTC GAATGC(1/-1)
BspHI 2922 TCATGA T^CATGA
BstEII 2993 GGTTACC G^GT[ACGT]ACC
BstPI 2993 GGTTACC G^GT[ACGT]ACC
DrdI 2886 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 38 CGGCCG C^GGCCG
EclXI 38 CGGCCG C^GGCCG
Eco47III 3832 AGCGCT AGC^GCT
Eco52I 38 CGGCCG C^GGCCG
EcoO65I 2993 GGTTACC G^GT[ACGT]ACC
HindIII 3297 AAGCTT A^AGCTT
MfeI 3259 CAATTG C^AATTG
MunI 3259 CAATTG C^AATTG
PmaCI 308 CACGTG CAC^GTG
PmlI 308 CACGTG CAC^GTG
RcaI 2922 TCATGA T^CATGA
SacI 3151 GAGCTC GAGCT^C
ScaI 4302 AGTACT AGT^ACT
SexAI 2648 ACCAGGT A^CC[AT]GGT
SmaI 1473 CCCGGG CCC^GGG
SphI 154 GCATGC GCATG^C
StuI 720 AGGCCT AGG^CCT
XmaCI 1473 CCCGGG C^CCGGG
XmaI 1473 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 1790 CTTAAG C^TTAAG
2720 CTTAAG
AocI 1563 CCTCAGG CC^T[ACGT]AGG
3659 CCTCAGG
BbvCI 2170 CCTCAGC CCTCAGC(-5/-2)
1415 GCTGAGG
BfrI 1790 CTTAAG C^TTAAG
2720 CTTAAG
BglI 2240 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2433 GCCTACCTGGC
BlpI 2533 GCTAAGC GC^T[ACGT]AGC
2691 GCTGAGC
Bpu1102I 2533 GCTAAGC GC^T[ACGT]AGC
2691 GCTGAGC
BsaBI 811 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1298 GATGATAATC
BsgI 575 CTGCAC GTGCAG(16/14)
1691 GTGCAG
BspLU11I 367 ACATGT A^CATGT
1687 ACATGT
BsrBI 66 GAGCGG CCGCTC(-3/-3)
714 GAGCGG
BssHII 33 GCGCGC G^CGCGC
3824 GCGCGC
Bsu36I 1563 CCTCAGG CC^T[ACGT]AGG
3659 CCTCAGG
CelII 2533 GCTAAGC GC^T[ACGT]AGC
2691 GCTGAGC
Eco81I 1563 CCTCAGG CC^T[ACGT]AGG
3659 CCTCAGG
EcoNI 1511 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2158 CCTCCTCCAGG
EcoRI 1210 GAATTC G^AATTC
4449 GAATTC
HgaI 2500 GACGC GACGC(5/10)
490 GCGTC
HincII 371 GTCAAC GT[CT]^[GA]AC
3387 GTTGAC
HindII 371 GTCAAC GT[CT]^[GA]AC
3387 GTTGAC
MamI 811 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1298 GATGATAATC
NaeI 2696 GCCGGC GCC^GGC
3515 GCCGGC
NcoI 1236 CCATGG C^CATGG
4071 CCATGG
NgoMIV 2696 GCCGGC G^CCGGC
3515 GCCGGC
PflMI 2998 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4263 CCACCTCGTGG
SapI 1772 GAAGAGC GCTCTTC(1/4)
3179 GAAGAGC
TaqI 966 TCGA T^CGA
1261 TCGA
TthHB8I 966 TCGA T^CGA
1261 TCGA
Van91I 2998 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4263 CCACCTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI