################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BbrPI BcgI BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrFI BssHII BssSI BstBI BstXI Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HincII HindII HpaI KpnI KspI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflII 129 CTTAAG C^TTAAG AocI 3803 CCTCAGG CC^T[ACGT]AGG AspI 215 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 2169 GGCGCC GGCGC^C BbvCI 4036 GCTGAGG CCTCAGC(-5/-2) BciVI 3190 GGATAC GTATCC(6/5) BclI 4002 TGATCA T^GATCA BfrI 129 CTTAAG C^TTAAG BglII 1073 AGATCT A^GATCT BlpI 2 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2 GCTCAGC GC^T[ACGT]AGC BsaAI 3047 CACGTA [CT]AC^GT[GA] BsgI 1791 CTGCAC GTGCAG(16/14) BsrBI 187 CCGCTC CCGCTC(-3/-3) BstEII 3815 GGTCACC G^GT[ACGT]ACC BstPI 3815 GGTCACC G^GT[ACGT]ACC Bsu36I 3803 CCTCAGG CC^T[ACGT]AGG CelII 2 GCTCAGC GC^T[ACGT]AGC DsaI 3885 CCATGG C^C[GA][CT]GG Eco81I 3803 CCTCAGG CC^T[ACGT]AGG EcoNI 2173 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3815 GGTCACC G^GT[ACGT]ACC FbaI 4002 TGATCA T^GATCA KasI 2169 GGCGCC G^GCGCC MfeI 4688 CAATTG C^AATTG MunI 4688 CAATTG C^AATTG NarI 2169 GGCGCC GG^CGCC NcoI 3885 CCATGG C^CATGG PflFI 215 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 600 GAGCTC GAGCT^C SapI 1424 GAAGAGC GCTCTTC(1/4) SfoI 2169 GGCGCC GGC^GCC SphI 4569 GCATGC GCATG^C Tth111I 215 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 218 GGCGTC G[GA]^CG[CT]C 2169 GGCGCC AseI 1508 ATTAAT AT^TAAT 4540 ATTAAT AsnI 1508 ATTAAT AT^TAAT 4540 ATTAAT AspHI 600 GAGCTC G[AT]GC[AT]^C 4303 GAGCAC AvaI 94 CTCGGG C^[CT]CG[GA]G 135 CCCGAG BalI 3386 TGGCCA TGG^CCA 4492 TGGCCA BamHI 3167 GGATCC G^GATCC 3700 GGATCC BsaHI 218 GGCGTC G[GA]^CG[CT]C 2169 GGCGCC BsaI 3611 GAGACC GGTCTC(1/5) 3095 GGTCTC BsaWI 153 TCCGGT [AT]^CCGG[AT] 363 ACCGGA BseRI 3464 CTCCTC GAGGAG(10/8) 4611 CTCCTC BsiHKAI 600 GAGCTC G[AT]GC[AT]^C 4303 GAGCAC BsoBI 94 CTCGGG C^[CT]CG[GA]G 135 CCCGAG Bsp1407I 1726 TGTACA T^GTACA 2869 TGTACA BspLU11I 793 ACATGT A^CATGT 3479 ACATGT BsrDI 4023 CATTGC GCAATG(2/0) 1737 GCAATG BsrGI 1726 TGTACA T^GTACA 2869 TGTACA Bst1107I 382 GTATAC GTA^TAC 1016 GTATAC BstAPI 3548 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4027 GCAGCCTTTGC BstZ17I 382 GTATAC GTA^TAC 1016 GTATAC DraII 57 AGGACCT [GA]G^G[ACGT]CC[CT] 85 GGGACCC EaeI 3386 TGGCCA [CT]^GGCC[GA] 4492 TGGCCA EcoO109I 57 AGGACCT [GA]G^G[ACGT]CC[CT] 85 GGGACCC EcoRI 2076 GAATTC G^AATTC 3378 GAATTC EcoT22I 1809 ATGCAT ATGCA^T 3693 ATGCAT HaeII 243 GGCGCT [GA]GCGC^[CT] 2169 GGCGCC Hin1I 218 GGCGTC G[GA]^CG[CT]C 2169 GGCGCC MaeII 3048 ACGT A^CGT 4307 ACGT MluNI 3386 TGGCCA TGG^CCA 4492 TGGCCA MscI 3386 TGGCCA TGG^CCA 4492 TGGCCA NsiI 1809 ATGCAT ATGCA^T 3693 ATGCAT PpuMI 57 AGGACCT [GA]G^G[AT]CC[CT] 85 GGGACCC PshBI 1508 ATTAAT AT^TAAT 4540 ATTAAT PstI 702 CTGCAG CTGCA^G 1077 CTGCAG SspBI 1726 TGTACA T^GTACA 2869 TGTACA XcmI 1525 CCAAGAAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3459 CCATACTCCTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DrdI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI SwaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI