##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BbrPI BcgI BlnI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrFI BssHII BssSI BstBI BstXI
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HincII HindII HpaI
KpnI KspI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 129 CTTAAG C^TTAAG
AocI 3803 CCTCAGG CC^T[ACGT]AGG
AspI 215 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2169 GGCGCC GGCGC^C
BbvCI 4036 GCTGAGG CCTCAGC(-5/-2)
BciVI 3190 GGATAC GTATCC(6/5)
BclI 4002 TGATCA T^GATCA
BfrI 129 CTTAAG C^TTAAG
BglII 1073 AGATCT A^GATCT
BlpI 2 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2 GCTCAGC GC^T[ACGT]AGC
BsaAI 3047 CACGTA [CT]AC^GT[GA]
BsgI 1791 CTGCAC GTGCAG(16/14)
BsrBI 187 CCGCTC CCGCTC(-3/-3)
BstEII 3815 GGTCACC G^GT[ACGT]ACC
BstPI 3815 GGTCACC G^GT[ACGT]ACC
Bsu36I 3803 CCTCAGG CC^T[ACGT]AGG
CelII 2 GCTCAGC GC^T[ACGT]AGC
DsaI 3885 CCATGG C^C[GA][CT]GG
Eco81I 3803 CCTCAGG CC^T[ACGT]AGG
EcoNI 2173 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3815 GGTCACC G^GT[ACGT]ACC
FbaI 4002 TGATCA T^GATCA
KasI 2169 GGCGCC G^GCGCC
MfeI 4688 CAATTG C^AATTG
MunI 4688 CAATTG C^AATTG
NarI 2169 GGCGCC GG^CGCC
NcoI 3885 CCATGG C^CATGG
PflFI 215 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 600 GAGCTC GAGCT^C
SapI 1424 GAAGAGC GCTCTTC(1/4)
SfoI 2169 GGCGCC GGC^GCC
SphI 4569 GCATGC GCATG^C
Tth111I 215 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 218 GGCGTC G[GA]^CG[CT]C
2169 GGCGCC
AseI 1508 ATTAAT AT^TAAT
4540 ATTAAT
AsnI 1508 ATTAAT AT^TAAT
4540 ATTAAT
AspHI 600 GAGCTC G[AT]GC[AT]^C
4303 GAGCAC
AvaI 94 CTCGGG C^[CT]CG[GA]G
135 CCCGAG
BalI 3386 TGGCCA TGG^CCA
4492 TGGCCA
BamHI 3167 GGATCC G^GATCC
3700 GGATCC
BsaHI 218 GGCGTC G[GA]^CG[CT]C
2169 GGCGCC
BsaI 3611 GAGACC GGTCTC(1/5)
3095 GGTCTC
BsaWI 153 TCCGGT [AT]^CCGG[AT]
363 ACCGGA
BseRI 3464 CTCCTC GAGGAG(10/8)
4611 CTCCTC
BsiHKAI 600 GAGCTC G[AT]GC[AT]^C
4303 GAGCAC
BsoBI 94 CTCGGG C^[CT]CG[GA]G
135 CCCGAG
Bsp1407I 1726 TGTACA T^GTACA
2869 TGTACA
BspLU11I 793 ACATGT A^CATGT
3479 ACATGT
BsrDI 4023 CATTGC GCAATG(2/0)
1737 GCAATG
BsrGI 1726 TGTACA T^GTACA
2869 TGTACA
Bst1107I 382 GTATAC GTA^TAC
1016 GTATAC
BstAPI 3548 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4027 GCAGCCTTTGC
BstZ17I 382 GTATAC GTA^TAC
1016 GTATAC
DraII 57 AGGACCT [GA]G^G[ACGT]CC[CT]
85 GGGACCC
EaeI 3386 TGGCCA [CT]^GGCC[GA]
4492 TGGCCA
EcoO109I 57 AGGACCT [GA]G^G[ACGT]CC[CT]
85 GGGACCC
EcoRI 2076 GAATTC G^AATTC
3378 GAATTC
EcoT22I 1809 ATGCAT ATGCA^T
3693 ATGCAT
HaeII 243 GGCGCT [GA]GCGC^[CT]
2169 GGCGCC
Hin1I 218 GGCGTC G[GA]^CG[CT]C
2169 GGCGCC
MaeII 3048 ACGT A^CGT
4307 ACGT
MluNI 3386 TGGCCA TGG^CCA
4492 TGGCCA
MscI 3386 TGGCCA TGG^CCA
4492 TGGCCA
NsiI 1809 ATGCAT ATGCA^T
3693 ATGCAT
PpuMI 57 AGGACCT [GA]G^G[AT]CC[CT]
85 GGGACCC
PshBI 1508 ATTAAT AT^TAAT
4540 ATTAAT
PstI 702 CTGCAG CTGCA^G
1077 CTGCAG
SspBI 1726 TGTACA T^GTACA
2869 TGTACA
XcmI 1525 CCAAGAAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3459 CCATACTCCTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaBI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DrdI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StyI SwaI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI