##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII BalI BbeI BbrPI BcgI
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I ClaI CpoI EagI
EclXI Eco47III Eco52I Eco81I EcoT22I
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NdeI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 4525 GTCTAC GT^[AC][GT]AC
AclI 4050 AACGTT AA^CGTT
AflII 3453 CTTAAG C^TTAAG
AflIII 3111 ACATGT A^C[GA][CT]GT
AhdI 1515 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1515 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2679 GGATCC G^GATCC
BbvCI 2986 CCTCAGC CCTCAGC(-5/-2)
BclI 1931 TGATCA T^GATCA
BfrI 3453 CTTAAG C^TTAAG
BglII 2090 AGATCT A^GATCT
BsaBI 3678 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 4078 CTGCAC GTGCAG(16/14)
BsmI 2895 GCATTC GAATGC(1/-1)
BspLU11I 3111 ACATGT A^CATGT
BsrFI 4139 GCCGGC [GA]^CCGG[CT]
BstAPI 63 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 345 GGTCACC G^GT[ACGT]ACC
BstPI 345 GGTCACC G^GT[ACGT]ACC
CfoI 2296 GCGC GCG^C
Cfr10I 4139 GCCGGC [GA]^CCGG[CT]
DrdI 4516 GACCTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1305 TGGCCG [CT]^GGCC[GA]
Eam1105I 1515 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 345 GGTCACC G^GT[ACGT]ACC
FauI 3449 CCCGC CCCGC(4/6)
FbaI 1931 TGATCA T^GATCA
HhaI 2296 GCGC GCG^C
HinP1I 2296 GCGC G^CGC
MamI 3678 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4139 GCCGGC GCC^GGC
NgoMIV 4139 GCCGGC G^CCGGC
PaeR7I 3928 CTCGAG C^TCGAG
Psp1406I 4050 AACGTT AA^CGTT
SfiI 1306 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 382 CCCGGG CCC^GGG
SpeI 3619 ACTAGT A^CTAGT
SphI 2209 GCATGC GCATG^C
XbaI 3920 TCTAGA T^CTAGA
XcmI 1052 CCAGCATGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3928 CTCGAG C^TCGAG
XmaCI 382 CCCGGG C^CCGGG
XmaI 382 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 1048 GGGCCC GGGCC^C
2281 GGGCCC
Asp700I 765 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1267 GAACAGCTTC
AvrII 3467 CCTAGG C^CTAGG
3481 CCTAGG
BanI 672 GGTGCC G^G[CT][GA]CC
2065 GGTGCC
BciVI 496 GGATAC GTATCC(6/5)
1905 GTATCC
BglI 1307 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2648 GCCTGGATGGC
BlnI 3467 CCTAGG C^CTAGG
3481 CCTAGG
BsrDI 3411 GCAATG GCAATG(2/0)
3796 GCAATG
BstXI 1669 CCAAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2860 CCAGGGGCTTGG
DraI 4055 TTTAAA TTT^AAA
4574 TTTAAA
DraIII 2145 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4178 CACAGGGTG
EcoRI 2260 GAATTC G^AATTC
4534 GAATTC
EcoRV 181 GATATC GAT^ATC
3993 GATATC
HgaI 405 GCGTC GACGC(5/10)
1604 GCGTC
HincII 2249 GTTGAC GT[CT]^[GA]AC
4197 GTCAAC
HindII 2249 GTTGAC GT[CT]^[GA]AC
4197 GTCAAC
HindIII 600 AAGCTT A^AGCTT
3400 AAGCTT
SbfI 2028 CCTGCAGG CCTGCA^GG
4518 CCTGCAGG
Sse8387I 2028 CCTGCAGG CCTGCA^GG
4518 CCTGCAGG
SspI 3306 AATATT AAT^ATT
3641 AATATT
TaqI 749 TCGA T^CGA
3929 TCGA
TthHB8I 749 TCGA T^CGA
3929 TCGA
XmnI 765 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1267 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII