##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AocI
Aor51HI AscI Asp718I AspEI AspI
AvrII BbeI BcgI BciVI BclI
BglII BlnI BsaBI BseAI BsiWI
BspDI BspEI BspLU11I BsrFI BssSI
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DrdI Eam1105I Eco47III
Eco81I FbaI FseI HindIII HpaI
KasI KpnI MamI MluI MroI
NaeI NarI NgoMIV NheI NruI
NspV PacI PflFI PinAI PmeI
PpuMI PshAI PvuI RsrII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1985 GACGTC GACGT^C
AclI 2333 AACGTT AA^CGTT
AflII 3149 CTTAAG C^TTAAG
Alw44I 118 GTGCAC G^TGCAC
ApaI 3363 GGGCCC GGGCC^C
ApaLI 118 GTGCAC G^TGCAC
AseI 819 ATTAAT AT^TAAT
AsnI 819 ATTAAT AT^TAAT
Asp700I 3062 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2932 TGGCCA TGG^CCA
BamHI 1496 GGATCC G^GATCC
BbrPI 2953 CACGTG CAC^GTG
BfrI 3149 CTTAAG C^TTAAG
BglI 3486 GCCCTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2953 CACGTG [CT]AC^GT[GA]
BsaWI 509 TCCGGT [AT]^CCGG[AT]
BsiEI 42 CGGCCG CG[GA][CT]^CG
BsmBI 3523 GAGACG CGTCTC(1/5)
BspHI 1529 TCATGA T^CATGA
BssHII 77 GCGCGC G^CGCGC
BstEII 1916 GGTGACC G^GT[ACGT]ACC
BstPI 1916 GGTGACC G^GT[ACGT]ACC
DraIII 573 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 42 CGGCCG C^GGCCG
EclXI 42 CGGCCG C^GGCCG
Eco52I 42 CGGCCG C^GGCCG
EcoO65I 1916 GGTGACC G^GT[ACGT]ACC
EcoRV 2894 GATATC GAT^ATC
EcoT22I 2702 ATGCAT ATGCA^T
MfeI 3633 CAATTG C^AATTG
MluNI 2932 TGGCCA TGG^CCA
MscI 2932 TGGCCA TGG^CCA
MunI 3633 CAATTG C^AATTG
NdeI 515 CATATG CA^TATG
NotI 41 GCGGCCGC GC^GGCCGC
NsiI 2702 ATGCAT ATGCA^T
PaeR7I 3489 CTCGAG C^TCGAG
PmaCI 2953 CACGTG CAC^GTG
PmlI 2953 CACGTG CAC^GTG
PshBI 819 ATTAAT AT^TAAT
Psp1406I 2333 AACGTT AA^CGTT
RcaI 1529 TCATGA T^CATGA
SacI 3206 GAGCTC GAGCT^C
SalI 3323 GTCGAC G^TCGAC
SspI 525 AATATT AAT^ATT
XhoI 3489 CTCGAG C^TCGAG
XmnI 3062 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 465 GTAGAC GT^[AC][GT]AC
3323 GTCGAC
AcyI 1985 GACGTC G[GA]^CG[CT]C
3320 GGCGTC
AflIII 2952 ACACGT A^C[GA][CT]GT
2954 ACGTGT
AviII 1591 TGCGCA TGC^GCA
3273 TGCGCA
BanI 320 GGCACC G^G[CT][GA]CC
3618 GGCACC
BbsI 1369 GAAGAC GAAGAC(2/6)
1802 GAAGAC
BpuAI 1369 GAAGAC GAAGAC(2/6)
1802 GAAGAC
BsaHI 1985 GACGTC G[GA]^CG[CT]C
3320 GGCGTC
BsmI 1505 GAATGC GAATGC(1/-1)
942 GCATTC
BsrBI 174 GAGCGG CCGCTC(-3/-3)
3176 GAGCGG
BsrDI 1907 CATTGC GCAATG(2/0)
261 GCAATG
BstAPI 1860 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2913 GCAGTGTTTGC
EaeI 42 CGGCCG [CT]^GGCC[GA]
2932 TGGCCA
EcoRI 490 GAATTC G^AATTC
2279 GAATTC
FspI 1591 TGCGCA TGC^GCA
3273 TGCGCA
HaeII 1307 AGCGCC [GA]GCGC^[CT]
2189 AGCGCC
Hin1I 1985 GACGTC G[GA]^CG[CT]C
3320 GGCGTC
HincII 1974 GTCAAC GT[CT]^[GA]AC
3323 GTCGAC
HindII 1974 GTCAAC GT[CT]^[GA]AC
3323 GTCGAC
NcoI 721 CCATGG C^CATGG
2341 CCATGG
PflMI 1469 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2024 CCAAATTGTGG
PvuII 1587 CAGCTG CAG^CTG
3696 CAGCTG
SapI 3241 GAAGAGC GCTCTTC(1/4)
3532 GAAGAGC
SmaI 57 CCCGGG CCC^GGG
369 CCCGGG
StuI 1034 AGGCCT AGG^CCT
2229 AGGCCT
Van91I 1469 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2024 CCAAATTGTGG
XbaI 616 TCTAGA T^CTAGA
2578 TCTAGA
XmaCI 57 CCCGGG C^CCGGG
369 CCCGGG
XmaI 57 CCCGGG C^CCGGG
369 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII