##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AviII BbrPI
BclI BglII BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DrdI Eco47III EcoRI FbaI FseI
FspI HpaI KpnI KspI MfeI
MluI MroI MunI MvnI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 4573 CTTAAG C^TTAAG
AhdI 3738 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4582 GTGCAC G^TGCAC
ApaLI 4582 GTGCAC G^TGCAC
AspEI 3738 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1945 CCTAGG C^CTAGG
BbeI 34 GGCGCC GGCGC^C
BcgI 407 GCAGTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4573 CTTAAG C^TTAAG
BlnI 1945 CCTAGG C^CTAGG
BsaBI 4659 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1006 CGGCCG CG[GA][CT]^CG
BspLU11I 440 ACATGT A^CATGT
BsrBI 38 CCGCTC CCGCTC(-3/-3)
BsrDI 4721 CATTGC GCAATG(2/0)
BstEII 300 GGTAACC G^GT[ACGT]ACC
BstPI 300 GGTAACC G^GT[ACGT]ACC
EagI 1006 CGGCCG C^GGCCG
Eam1105I 3738 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1006 CGGCCG C^GGCCG
Eco52I 1006 CGGCCG C^GGCCG
EcoO65I 300 GGTAACC G^GT[ACGT]ACC
EcoRV 1090 GATATC GAT^ATC
EcoT22I 322 ATGCAT ATGCA^T
FauI 1434 CCCGC CCCGC(4/6)
HgaI 489 GACGC GACGC(5/10)
HincII 1665 GTTGAC GT[CT]^[GA]AC
HindII 1665 GTTGAC GT[CT]^[GA]AC
HindIII 3553 AAGCTT A^AGCTT
KasI 34 GGCGCC G^GCGCC
MamI 4659 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 34 GGCGCC GG^CGCC
NdeI 731 CATATG CA^TATG
NheI 7 GCTAGC G^CTAGC
NsiI 322 ATGCAT ATGCA^T
PmeI 4626 GTTTAAAC GTTT^AAAC
SbfI 1245 CCTGCAGG CCTGCA^GG
SfoI 34 GGCGCC GGC^GCC
Sse8387I 1245 CCTGCAGG CCTGCA^GG
XbaI 4225 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 3797 AAATTC [GA]^AATT[CT]
4820 AAATTC
AcyI 34 GGCGCC G[GA]^CG[CT]C
489 GACGCC
AflIII 440 ACATGT A^C[GA][CT]GT
2228 ACACGT
AocI 1567 CCTGAGG CC^T[ACGT]AGG
2125 CCTGAGG
ApoI 3797 AAATTC [GA]^AATT[CT]
4820 AAATTC
AspI 1937 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2087 GACCAAGTC
BalI 2809 TGGCCA TGG^CCA
4082 TGGCCA
BamHI 2130 GGATCC G^GATCC
4595 GGATCC
BbvCI 1392 CCTCAGC CCTCAGC(-5/-2)
2874 GCTGAGG
BglI 1440 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1529 GCCTTAGTGGC
BsaHI 34 GGCGCC G[GA]^CG[CT]C
489 GACGCC
BsrFI 988 GCCGGC [GA]^CCGG[CT]
1004 GCCGGC
Bsu36I 1567 CCTGAGG CC^T[ACGT]AGG
2125 CCTGAGG
Cfr10I 988 GCCGGC [GA]^CCGG[CT]
1004 GCCGGC
DraIII 818 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3892 CACTTTGTG
Eco81I 1567 CCTGAGG CC^T[ACGT]AGG
2125 CCTGAGG
EcoNI 291 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1904 CCTGGATAAGG
Hin1I 34 GGCGCC G[GA]^CG[CT]C
489 GACGCC
MaeII 2230 ACGT A^CGT
2324 ACGT
MluNI 2809 TGGCCA TGG^CCA
4082 TGGCCA
MscI 2809 TGGCCA TGG^CCA
4082 TGGCCA
NaeI 988 GCCGGC GCC^GGC
1004 GCCGGC
NgoMIV 988 GCCGGC G^CCGGC
1004 GCCGGC
PflFI 1937 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2087 GACCAAGTC
SapI 872 GCTCTTC GCTCTTC(1/4)
3082 GCTCTTC
SspI 4383 AATATT AAT^ATT
4750 AATATT
Tth111I 1937 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2087 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI