################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AviII BbrPI BclI BglII BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BssHII Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DrdI Eco47III EcoRI FbaI FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI MvnI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SwaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflII 4573 CTTAAG C^TTAAG AhdI 3738 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4582 GTGCAC G^TGCAC ApaLI 4582 GTGCAC G^TGCAC AspEI 3738 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1945 CCTAGG C^CTAGG BbeI 34 GGCGCC GGCGC^C BcgI 407 GCAGTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4573 CTTAAG C^TTAAG BlnI 1945 CCTAGG C^CTAGG BsaBI 4659 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1006 CGGCCG CG[GA][CT]^CG BspLU11I 440 ACATGT A^CATGT BsrBI 38 CCGCTC CCGCTC(-3/-3) BsrDI 4721 CATTGC GCAATG(2/0) BstEII 300 GGTAACC G^GT[ACGT]ACC BstPI 300 GGTAACC G^GT[ACGT]ACC EagI 1006 CGGCCG C^GGCCG Eam1105I 3738 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1006 CGGCCG C^GGCCG Eco52I 1006 CGGCCG C^GGCCG EcoO65I 300 GGTAACC G^GT[ACGT]ACC EcoRV 1090 GATATC GAT^ATC EcoT22I 322 ATGCAT ATGCA^T FauI 1434 CCCGC CCCGC(4/6) HgaI 489 GACGC GACGC(5/10) HincII 1665 GTTGAC GT[CT]^[GA]AC HindII 1665 GTTGAC GT[CT]^[GA]AC HindIII 3553 AAGCTT A^AGCTT KasI 34 GGCGCC G^GCGCC MamI 4659 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 34 GGCGCC GG^CGCC NdeI 731 CATATG CA^TATG NheI 7 GCTAGC G^CTAGC NsiI 322 ATGCAT ATGCA^T PmeI 4626 GTTTAAAC GTTT^AAAC SbfI 1245 CCTGCAGG CCTGCA^GG SfoI 34 GGCGCC GGC^GCC Sse8387I 1245 CCTGCAGG CCTGCA^GG XbaI 4225 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 3797 AAATTC [GA]^AATT[CT] 4820 AAATTC AcyI 34 GGCGCC G[GA]^CG[CT]C 489 GACGCC AflIII 440 ACATGT A^C[GA][CT]GT 2228 ACACGT AocI 1567 CCTGAGG CC^T[ACGT]AGG 2125 CCTGAGG ApoI 3797 AAATTC [GA]^AATT[CT] 4820 AAATTC AspI 1937 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2087 GACCAAGTC BalI 2809 TGGCCA TGG^CCA 4082 TGGCCA BamHI 2130 GGATCC G^GATCC 4595 GGATCC BbvCI 1392 CCTCAGC CCTCAGC(-5/-2) 2874 GCTGAGG BglI 1440 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1529 GCCTTAGTGGC BsaHI 34 GGCGCC G[GA]^CG[CT]C 489 GACGCC BsrFI 988 GCCGGC [GA]^CCGG[CT] 1004 GCCGGC Bsu36I 1567 CCTGAGG CC^T[ACGT]AGG 2125 CCTGAGG Cfr10I 988 GCCGGC [GA]^CCGG[CT] 1004 GCCGGC DraIII 818 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG 3892 CACTTTGTG Eco81I 1567 CCTGAGG CC^T[ACGT]AGG 2125 CCTGAGG EcoNI 291 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1904 CCTGGATAAGG Hin1I 34 GGCGCC G[GA]^CG[CT]C 489 GACGCC MaeII 2230 ACGT A^CGT 2324 ACGT MluNI 2809 TGGCCA TGG^CCA 4082 TGGCCA MscI 2809 TGGCCA TGG^CCA 4082 TGGCCA NaeI 988 GCCGGC GCC^GGC 1004 GCCGGC NgoMIV 988 GCCGGC G^CCGGC 1004 GCCGGC PflFI 1937 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2087 GACCAAGTC SapI 872 GCTCTTC GCTCTTC(1/4) 3082 GCTCTTC SspI 4383 AATATT AAT^ATT 4750 AATATT Tth111I 1937 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2087 GACCAAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI