################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbrPI BcgI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiWI BsmBI BspEI BspHI BspMI BsrDI BssSI BstBI CelII CpoI DrdI Eco47III EcoNI EcoRV FseI FspI HpaI KpnI MamI MroI NcoI NheI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SnaBI SpeI Sse8387I StuI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 3024 GTATAC GT^[AC][GT]AC AclI 1383 AACGTT AA^CGTT AhdI 922 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1603 CCTGAGG CC^T[ACGT]AGG AspEI 922 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 816 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1915 GGACC G^G[AT]CC BalI 1013 TGGCCA TGG^CCA BbvCI 1006 CCTCAGC CCTCAGC(-5/-2) BclI 1896 TGATCA T^GATCA BglII 846 AGATCT A^GATCT BsaAI 1324 TACGTG [CT]AC^GT[GA] BspDI 946 ATCGAT AT^CGAT Bst1107I 3024 GTATAC GTA^TAC BstAPI 335 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2371 GGTAACC G^GT[ACGT]ACC BstF5I 210 CATCC GGATG(2/0) BstPI 2371 GGTAACC G^GT[ACGT]ACC BstXI 1733 CCAACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3024 GTATAC GTA^TAC Bsu36I 1603 CCTGAGG CC^T[ACGT]AGG ClaI 946 ATCGAT AT^CGAT DraIII 336 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 922 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1603 CCTGAGG CC^T[ACGT]AGG EcoO65I 2371 GGTAACC G^GT[ACGT]ACC EcoRI 1796 GAATTC G^AATTC FbaI 1896 TGATCA T^GATCA FokI 210 CATCC GGATG(9/13) HincII 885 GTTGAC GT[CT]^[GA]AC HindII 885 GTTGAC GT[CT]^[GA]AC HindIII 4028 AAGCTT A^AGCTT KspI 629 CCGCGG CCGC^GG MluI 593 ACGCGT A^CGCGT MluNI 1013 TGGCCA TGG^CCA MscI 1013 TGGCCA TGG^CCA PflFI 816 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 1383 AACGTT AA^CGTT SacI 21 GAGCTC GAGCT^C SacII 629 CCGCGG CCGC^GG SgrAI 231 CGCCGGCG C[GA]^CCGG[CT]G SphI 2749 GCATGC GCATG^C SwaI 3520 ATTTAAAT ATTT^AAAT Tth111I 816 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### ApaI 435 GGGCCC GGGCC^C 564 GGGCCC BanI 229 GGCGCC G^G[CT][GA]CC 347 GGCGCC BbeI 229 GGCGCC GGCGC^C 347 GGCGCC BciVI 1481 GGATAC GTATCC(6/5) 3655 GTATCC BglI 350 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1549 GCCACCTTGGC BsaI 586 GAGACC GGTCTC(1/5) 1757 GGTCTC BssHII 318 GCGCGC G^CGCGC 320 GCGCGC BstYI 473 AGATCC [GA]^GATC[CT] 846 AGATCT DsaI 406 CCGTGG C^C[GA][CT]GG 629 CCGCGG EcoT14I 569 CCAAGG C^C[AT][AT]GG 1553 CCTTGG HaeII 229 GGCGCC [GA]GCGC^[CT] 347 GGCGCC KasI 229 GGCGCC G^GCGCC 347 GGCGCC MflI 473 AGATCC [GA]^GATC[CT] 846 AGATCT NarI 229 GGCGCC GG^CGCC 347 GGCGCC NdeI 2579 CATATG CA^TATG 3578 CATATG NotI 190 GCGGCCGC GC^GGCCGC 422 GCGGCCGC PflMI 1576 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4036 CCAGTTTGTGG ScaI 2488 AGTACT AGT^ACT 2546 AGTACT SfoI 229 GGCGCC GGC^GCC 347 GGCGCC StyI 569 CCAAGG C^C[AT][AT]GG 1553 CCTTGG Van91I 1576 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4036 CCAGTTTGTGG XhoII 473 AGATCC [GA]^GATC[CT] 846 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AvaI BanII BbsI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BsrBI BsrFI BsrGI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT22I FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI