##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbrPI BcgI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BspEI BspHI BspMI BsrDI
BssSI BstBI CelII CpoI DrdI
Eco47III EcoNI EcoRV FseI FspI
HpaI KpnI MamI MroI NcoI
NheI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RcaI RsrII
SalI SapI SbfI SexAI SfiI
SfuI SnaBI SpeI Sse8387I StuI
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 3024 GTATAC GT^[AC][GT]AC
AclI 1383 AACGTT AA^CGTT
AhdI 922 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1603 CCTGAGG CC^T[ACGT]AGG
AspEI 922 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 816 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1915 GGACC G^G[AT]CC
BalI 1013 TGGCCA TGG^CCA
BbvCI 1006 CCTCAGC CCTCAGC(-5/-2)
BclI 1896 TGATCA T^GATCA
BglII 846 AGATCT A^GATCT
BsaAI 1324 TACGTG [CT]AC^GT[GA]
BspDI 946 ATCGAT AT^CGAT
Bst1107I 3024 GTATAC GTA^TAC
BstAPI 335 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2371 GGTAACC G^GT[ACGT]ACC
BstF5I 210 CATCC GGATG(2/0)
BstPI 2371 GGTAACC G^GT[ACGT]ACC
BstXI 1733 CCAACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3024 GTATAC GTA^TAC
Bsu36I 1603 CCTGAGG CC^T[ACGT]AGG
ClaI 946 ATCGAT AT^CGAT
DraIII 336 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 922 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1603 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2371 GGTAACC G^GT[ACGT]ACC
EcoRI 1796 GAATTC G^AATTC
FbaI 1896 TGATCA T^GATCA
FokI 210 CATCC GGATG(9/13)
HincII 885 GTTGAC GT[CT]^[GA]AC
HindII 885 GTTGAC GT[CT]^[GA]AC
HindIII 4028 AAGCTT A^AGCTT
KspI 629 CCGCGG CCGC^GG
MluI 593 ACGCGT A^CGCGT
MluNI 1013 TGGCCA TGG^CCA
MscI 1013 TGGCCA TGG^CCA
PflFI 816 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1383 AACGTT AA^CGTT
SacI 21 GAGCTC GAGCT^C
SacII 629 CCGCGG CCGC^GG
SgrAI 231 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2749 GCATGC GCATG^C
SwaI 3520 ATTTAAAT ATTT^AAAT
Tth111I 816 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 435 GGGCCC GGGCC^C
564 GGGCCC
BanI 229 GGCGCC G^G[CT][GA]CC
347 GGCGCC
BbeI 229 GGCGCC GGCGC^C
347 GGCGCC
BciVI 1481 GGATAC GTATCC(6/5)
3655 GTATCC
BglI 350 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1549 GCCACCTTGGC
BsaI 586 GAGACC GGTCTC(1/5)
1757 GGTCTC
BssHII 318 GCGCGC G^CGCGC
320 GCGCGC
BstYI 473 AGATCC [GA]^GATC[CT]
846 AGATCT
DsaI 406 CCGTGG C^C[GA][CT]GG
629 CCGCGG
EcoT14I 569 CCAAGG C^C[AT][AT]GG
1553 CCTTGG
HaeII 229 GGCGCC [GA]GCGC^[CT]
347 GGCGCC
KasI 229 GGCGCC G^GCGCC
347 GGCGCC
MflI 473 AGATCC [GA]^GATC[CT]
846 AGATCT
NarI 229 GGCGCC GG^CGCC
347 GGCGCC
NdeI 2579 CATATG CA^TATG
3578 CATATG
NotI 190 GCGGCCGC GC^GGCCGC
422 GCGGCCGC
PflMI 1576 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4036 CCAGTTTGTGG
ScaI 2488 AGTACT AGT^ACT
2546 AGTACT
SfoI 229 GGCGCC GGC^GCC
347 GGCGCC
StyI 569 CCAAGG C^C[AT][AT]GG
1553 CCTTGG
Van91I 1576 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4036 CCAGTTTGTGG
XhoII 473 AGATCC [GA]^GATC[CT]
846 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AspHI AvaI BanII BbsI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BsrBI BsrFI BsrGI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT22I FauI
Fnu4HI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI