##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

Acc65I      AccIII      AclI        ApaI        AscI        
Asp718I     AspI        AviII       BalI        BamHI       
BbrPI       BglII       BseAI       BsiWI       BspEI       
BsrBI       BssHII      BstBI       BstEII      BstPI       
CpoI        DraIII      DrdI        EaeI        EagI        
EclXI       Eco52I      EcoO65I     EcoRV       FseI        
FspI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
Tth111I     Van91I      XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        1274  GACGTC               GACGT^C
AgeI         2213  ACCGGT               A^CCGGT
AhdI         1016  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1180  GTGCAC               G^TGCAC
Aor51HI       716  AGCGCT               AGC^GCT
ApaLI        1180  GTGCAC               G^TGCAC
AspEI        1016  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         2949  CCCGGG               C^[CT]CG[GA]G
AvrII        2635  CCTAGG               C^CTAGG
BbeI          342  GGCGCC               GGCGC^C
BcgI          938  GCAGCAAGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         560  GTATCC               GTATCC(6/5)
BclI         1667  TGATCA               T^GATCA
BglI          690  GCCTATCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2635  CCTAGG               C^CTAGG
BsaAI         762  TACGTG               [CT]AC^GT[GA]
BsaBI          99  GATGAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2213  ACCGGT               [AT]^CCGG[AT]
BsiEI        1609  CGACCG               CG[GA][CT]^CG
BsoBI        2949  CCCGGG               C^[CT]CG[GA]G
BspDI          96  ATCGAT               AT^CGAT
BspHI        1881  TCATGA               T^CATGA
BsrFI        2213  ACCGGT               [GA]^CCGG[CT]
BssSI        4112  CTCGTG               CACGAG(-5/-1)
BstXI        3797  CCAGATGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       2213  ACCGGT               [GA]^CCGG[CT]
ClaI           96  ATCGAT               AT^CGAT
Eam1105I     1016  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      716  AGCGCT               AGC^GCT
EcoRI        4121  GAATTC               G^AATTC
FbaI         1667  TGATCA               T^GATCA
HincII       3723  GTTAAC               GT[CT]^[GA]AC
HindII       3723  GTTAAC               GT[CT]^[GA]AC
HpaI         3723  GTTAAC               GTT^AAC
KasI          342  GGCGCC               G^GCGCC
MamI           99  GATGAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          342  GGCGCC               GG^CGCC
NheI         4309  GCTAGC               G^CTAGC
PinAI        2213  ACCGGT               A^CCGGT
PpuMI         916  AGGACCC              [GA]G^G[AT]CC[CT]
RcaI         1881  TCATGA               T^CATGA
ScaI         1767  AGTACT               AGT^ACT
SexAI         199  ACCTGGT              A^CC[AT]GGT
SfoI          342  GGCGCC               GGC^GCC
SmaI         2949  CCCGGG               CCC^GGG
XmaCI        2949  CCCGGG               C^CCGGG
XmaI         2949  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   44 enzymes) #
###################################

AccI         2109  GTATAC               GT^[AC][GT]AC
             3552  GTATAC              
AccII        1580  CGCG                 CG^CG
             3329  CGCG                
AcyI          342  GGCGCC               G[GA]^CG[CT]C
             1274  GACGTC              
AflII        2358  CTTAAG               C^TTAAG
             4707  CTTAAG              
AlwNI         810  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2544  CAGACCCTG           
AocI          912  CCTCAGG              CC^T[ACGT]AGG
             3457  CCTAAGG             
Asp700I      3941  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4746  GAATCAGTTC          
BbvCI         213  CCTCAGC              CCTCAGC(-5/-2)
              546  CCTCAGC             
BcnI         2949  CCCGG                CC^[GC]GG
             2950  CCGGG               
BfrI         2358  CTTAAG               C^TTAAG
             4707  CTTAAG              
BlpI            9  GCTGAGC              GC^T[ACGT]AGC
              639  GCTCAGC             
Bpu1102I        9  GCTGAGC              GC^T[ACGT]AGC
              639  GCTCAGC             
BsaHI         342  GGCGCC               G[GA]^CG[CT]C
             1274  GACGTC              
BsaI         1035  GGTCTC               GGTCTC(1/5)
             1575  GGTCTC              
BseRI         963  CTCCTC               GAGGAG(10/8)
             2932  CTCCTC              
Bsp1407I     2730  TGTACA               T^GTACA
             4294  TGTACA              
BsrDI        2650  CATTGC               GCAATG(2/0)
             1044  GCAATG              
BsrGI        2730  TGTACA               T^GTACA
             4294  TGTACA              
Bst1107I     2109  GTATAC               GTA^TAC
             3552  GTATAC              
BstAPI        386  GCAACAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1076  GCAAGGCTTGC         
BstUI        1580  CGCG                 CG^CG
             3329  CGCG                
BstYI        2445  GGATCT               [GA]^GATC[CT]
             2682  AGATCC              
BstZ17I      2109  GTATAC               GTA^TAC
             3552  GTATAC              
Bsu36I        912  CCTCAGG              CC^T[ACGT]AGG
             3457  CCTAAGG             
CelII           9  GCTGAGC              GC^T[ACGT]AGC
              639  GCTCAGC             
Eco81I        912  CCTCAGG              CC^T[ACGT]AGG
             3457  CCTAAGG             
EcoNI         908  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4171  CCTGAGCAAGG         
EcoT22I      2395  ATGCAT               ATGCA^T
             3431  ATGCAT              
HaeII         342  GGCGCC               [GA]GCGC^[CT]
              716  AGCGCT              
HgaI          334  GACGC                GACGC(5/10)
              864  GACGC               
Hin1I         342  GGCGCC               G[GA]^CG[CT]C
             1274  GACGTC              
HindIII       310  AAGCTT               A^AGCTT
              405  AAGCTT              
MflI         2445  GGATCT               [GA]^GATC[CT]
             2682  AGATCC              
MvnI         1580  CGCG                 CG^CG
             3329  CGCG                
NciI         2949  CCCGG                CC^[GC]GG
             2950  CCGGG               
NsiI         2395  ATGCAT               ATGCA^T
             3431  ATGCAT              
SacI           86  GAGCTC               GAGCT^C
             3314  GAGCTC              
SapI         2538  GAAGAGC              GCTCTTC(1/4)
             1599  GCTCTTC             
SspBI        2730  TGTACA               T^GTACA
             4294  TGTACA              
StuI          775  AGGCCT               AGG^CCT
             1411  AGGCCT              
SwaI         1340  ATTTAAAT             ATTT^AAAT
             4067  ATTTAAAT            
XbaI         3949  TCTAGA               T^CTAGA
             4106  TCTAGA              
XhoII        2445  GGATCT               [GA]^GATC[CT]
             2682  AGATCC              
XmnI         3941  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4746  GAATCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AseI        AsnI        AspHI       
AvaII       BanI        BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        Bsp1286I    BspLU11I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshBI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I