################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AccIII AclI ApaI AscI Asp718I AspI AviII BalI BamHI BbrPI BglII BseAI BsiWI BspEI BsrBI BssHII BstBI BstEII BstPI CpoI DraIII DrdI EaeI EagI EclXI Eco52I EcoO65I EcoRV FseI FspI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I Tth111I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1274 GACGTC GACGT^C AgeI 2213 ACCGGT A^CCGGT AhdI 1016 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1180 GTGCAC G^TGCAC Aor51HI 716 AGCGCT AGC^GCT ApaLI 1180 GTGCAC G^TGCAC AspEI 1016 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2949 CCCGGG C^[CT]CG[GA]G AvrII 2635 CCTAGG C^CTAGG BbeI 342 GGCGCC GGCGC^C BcgI 938 GCAGCAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 560 GTATCC GTATCC(6/5) BclI 1667 TGATCA T^GATCA BglI 690 GCCTATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2635 CCTAGG C^CTAGG BsaAI 762 TACGTG [CT]AC^GT[GA] BsaBI 99 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2213 ACCGGT [AT]^CCGG[AT] BsiEI 1609 CGACCG CG[GA][CT]^CG BsoBI 2949 CCCGGG C^[CT]CG[GA]G BspDI 96 ATCGAT AT^CGAT BspHI 1881 TCATGA T^CATGA BsrFI 2213 ACCGGT [GA]^CCGG[CT] BssSI 4112 CTCGTG CACGAG(-5/-1) BstXI 3797 CCAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 2213 ACCGGT [GA]^CCGG[CT] ClaI 96 ATCGAT AT^CGAT Eam1105I 1016 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 716 AGCGCT AGC^GCT EcoRI 4121 GAATTC G^AATTC FbaI 1667 TGATCA T^GATCA HincII 3723 GTTAAC GT[CT]^[GA]AC HindII 3723 GTTAAC GT[CT]^[GA]AC HpaI 3723 GTTAAC GTT^AAC KasI 342 GGCGCC G^GCGCC MamI 99 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 342 GGCGCC GG^CGCC NheI 4309 GCTAGC G^CTAGC PinAI 2213 ACCGGT A^CCGGT PpuMI 916 AGGACCC [GA]G^G[AT]CC[CT] RcaI 1881 TCATGA T^CATGA ScaI 1767 AGTACT AGT^ACT SexAI 199 ACCTGGT A^CC[AT]GGT SfoI 342 GGCGCC GGC^GCC SmaI 2949 CCCGGG CCC^GGG XmaCI 2949 CCCGGG C^CCGGG XmaI 2949 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 44 enzymes) # ################################### AccI 2109 GTATAC GT^[AC][GT]AC 3552 GTATAC AccII 1580 CGCG CG^CG 3329 CGCG AcyI 342 GGCGCC G[GA]^CG[CT]C 1274 GACGTC AflII 2358 CTTAAG C^TTAAG 4707 CTTAAG AlwNI 810 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 2544 CAGACCCTG AocI 912 CCTCAGG CC^T[ACGT]AGG 3457 CCTAAGG Asp700I 3941 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4746 GAATCAGTTC BbvCI 213 CCTCAGC CCTCAGC(-5/-2) 546 CCTCAGC BcnI 2949 CCCGG CC^[GC]GG 2950 CCGGG BfrI 2358 CTTAAG C^TTAAG 4707 CTTAAG BlpI 9 GCTGAGC GC^T[ACGT]AGC 639 GCTCAGC Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC 639 GCTCAGC BsaHI 342 GGCGCC G[GA]^CG[CT]C 1274 GACGTC BsaI 1035 GGTCTC GGTCTC(1/5) 1575 GGTCTC BseRI 963 CTCCTC GAGGAG(10/8) 2932 CTCCTC Bsp1407I 2730 TGTACA T^GTACA 4294 TGTACA BsrDI 2650 CATTGC GCAATG(2/0) 1044 GCAATG BsrGI 2730 TGTACA T^GTACA 4294 TGTACA Bst1107I 2109 GTATAC GTA^TAC 3552 GTATAC BstAPI 386 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1076 GCAAGGCTTGC BstUI 1580 CGCG CG^CG 3329 CGCG BstYI 2445 GGATCT [GA]^GATC[CT] 2682 AGATCC BstZ17I 2109 GTATAC GTA^TAC 3552 GTATAC Bsu36I 912 CCTCAGG CC^T[ACGT]AGG 3457 CCTAAGG CelII 9 GCTGAGC GC^T[ACGT]AGC 639 GCTCAGC Eco81I 912 CCTCAGG CC^T[ACGT]AGG 3457 CCTAAGG EcoNI 908 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4171 CCTGAGCAAGG EcoT22I 2395 ATGCAT ATGCA^T 3431 ATGCAT HaeII 342 GGCGCC [GA]GCGC^[CT] 716 AGCGCT HgaI 334 GACGC GACGC(5/10) 864 GACGC Hin1I 342 GGCGCC G[GA]^CG[CT]C 1274 GACGTC HindIII 310 AAGCTT A^AGCTT 405 AAGCTT MflI 2445 GGATCT [GA]^GATC[CT] 2682 AGATCC MvnI 1580 CGCG CG^CG 3329 CGCG NciI 2949 CCCGG CC^[GC]GG 2950 CCGGG NsiI 2395 ATGCAT ATGCA^T 3431 ATGCAT SacI 86 GAGCTC GAGCT^C 3314 GAGCTC SapI 2538 GAAGAGC GCTCTTC(1/4) 1599 GCTCTTC SspBI 2730 TGTACA T^GTACA 4294 TGTACA StuI 775 AGGCCT AGG^CCT 1411 AGGCCT SwaI 1340 ATTTAAAT ATTT^AAAT 4067 ATTTAAAT XbaI 3949 TCTAGA T^CTAGA 4106 TCTAGA XhoII 2445 GGATCT [GA]^GATC[CT] 2682 AGATCC XmnI 3941 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4746 GAATCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I