##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AclI ApaI AscI
Asp718I AspI AviII BalI BamHI
BbrPI BglII BseAI BsiWI BspEI
BsrBI BssHII BstBI BstEII BstPI
CpoI DraIII DrdI EaeI EagI
EclXI Eco52I EcoO65I EcoRV FseI
FspI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
Tth111I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1274 GACGTC GACGT^C
AgeI 2213 ACCGGT A^CCGGT
AhdI 1016 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1180 GTGCAC G^TGCAC
Aor51HI 716 AGCGCT AGC^GCT
ApaLI 1180 GTGCAC G^TGCAC
AspEI 1016 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2949 CCCGGG C^[CT]CG[GA]G
AvrII 2635 CCTAGG C^CTAGG
BbeI 342 GGCGCC GGCGC^C
BcgI 938 GCAGCAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 560 GTATCC GTATCC(6/5)
BclI 1667 TGATCA T^GATCA
BglI 690 GCCTATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2635 CCTAGG C^CTAGG
BsaAI 762 TACGTG [CT]AC^GT[GA]
BsaBI 99 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2213 ACCGGT [AT]^CCGG[AT]
BsiEI 1609 CGACCG CG[GA][CT]^CG
BsoBI 2949 CCCGGG C^[CT]CG[GA]G
BspDI 96 ATCGAT AT^CGAT
BspHI 1881 TCATGA T^CATGA
BsrFI 2213 ACCGGT [GA]^CCGG[CT]
BssSI 4112 CTCGTG CACGAG(-5/-1)
BstXI 3797 CCAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2213 ACCGGT [GA]^CCGG[CT]
ClaI 96 ATCGAT AT^CGAT
Eam1105I 1016 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 716 AGCGCT AGC^GCT
EcoRI 4121 GAATTC G^AATTC
FbaI 1667 TGATCA T^GATCA
HincII 3723 GTTAAC GT[CT]^[GA]AC
HindII 3723 GTTAAC GT[CT]^[GA]AC
HpaI 3723 GTTAAC GTT^AAC
KasI 342 GGCGCC G^GCGCC
MamI 99 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 342 GGCGCC GG^CGCC
NheI 4309 GCTAGC G^CTAGC
PinAI 2213 ACCGGT A^CCGGT
PpuMI 916 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 1881 TCATGA T^CATGA
ScaI 1767 AGTACT AGT^ACT
SexAI 199 ACCTGGT A^CC[AT]GGT
SfoI 342 GGCGCC GGC^GCC
SmaI 2949 CCCGGG CCC^GGG
XmaCI 2949 CCCGGG C^CCGGG
XmaI 2949 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccI 2109 GTATAC GT^[AC][GT]AC
3552 GTATAC
AccII 1580 CGCG CG^CG
3329 CGCG
AcyI 342 GGCGCC G[GA]^CG[CT]C
1274 GACGTC
AflII 2358 CTTAAG C^TTAAG
4707 CTTAAG
AlwNI 810 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2544 CAGACCCTG
AocI 912 CCTCAGG CC^T[ACGT]AGG
3457 CCTAAGG
Asp700I 3941 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4746 GAATCAGTTC
BbvCI 213 CCTCAGC CCTCAGC(-5/-2)
546 CCTCAGC
BcnI 2949 CCCGG CC^[GC]GG
2950 CCGGG
BfrI 2358 CTTAAG C^TTAAG
4707 CTTAAG
BlpI 9 GCTGAGC GC^T[ACGT]AGC
639 GCTCAGC
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
639 GCTCAGC
BsaHI 342 GGCGCC G[GA]^CG[CT]C
1274 GACGTC
BsaI 1035 GGTCTC GGTCTC(1/5)
1575 GGTCTC
BseRI 963 CTCCTC GAGGAG(10/8)
2932 CTCCTC
Bsp1407I 2730 TGTACA T^GTACA
4294 TGTACA
BsrDI 2650 CATTGC GCAATG(2/0)
1044 GCAATG
BsrGI 2730 TGTACA T^GTACA
4294 TGTACA
Bst1107I 2109 GTATAC GTA^TAC
3552 GTATAC
BstAPI 386 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1076 GCAAGGCTTGC
BstUI 1580 CGCG CG^CG
3329 CGCG
BstYI 2445 GGATCT [GA]^GATC[CT]
2682 AGATCC
BstZ17I 2109 GTATAC GTA^TAC
3552 GTATAC
Bsu36I 912 CCTCAGG CC^T[ACGT]AGG
3457 CCTAAGG
CelII 9 GCTGAGC GC^T[ACGT]AGC
639 GCTCAGC
Eco81I 912 CCTCAGG CC^T[ACGT]AGG
3457 CCTAAGG
EcoNI 908 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4171 CCTGAGCAAGG
EcoT22I 2395 ATGCAT ATGCA^T
3431 ATGCAT
HaeII 342 GGCGCC [GA]GCGC^[CT]
716 AGCGCT
HgaI 334 GACGC GACGC(5/10)
864 GACGC
Hin1I 342 GGCGCC G[GA]^CG[CT]C
1274 GACGTC
HindIII 310 AAGCTT A^AGCTT
405 AAGCTT
MflI 2445 GGATCT [GA]^GATC[CT]
2682 AGATCC
MvnI 1580 CGCG CG^CG
3329 CGCG
NciI 2949 CCCGG CC^[GC]GG
2950 CCGGG
NsiI 2395 ATGCAT ATGCA^T
3431 ATGCAT
SacI 86 GAGCTC GAGCT^C
3314 GAGCTC
SapI 2538 GAAGAGC GCTCTTC(1/4)
1599 GCTCTTC
SspBI 2730 TGTACA T^GTACA
4294 TGTACA
StuI 775 AGGCCT AGG^CCT
1411 AGGCCT
SwaI 1340 ATTTAAAT ATTT^AAAT
4067 ATTTAAAT
XbaI 3949 TCTAGA T^CTAGA
4106 TCTAGA
XhoII 2445 GGATCT [GA]^GATC[CT]
2682 AGATCC
XmnI 3941 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4746 GAATCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I