##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI Asp718I
AspEI AvaI AviII AvrII BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfrI BglI BlnI BpuAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 2122 GTGCAC G^TGCAC
ApaLI 2122 GTGCAC G^TGCAC
AspI 1063 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2616 GGATCC G^GATCC
BanI 518 GGCACC G^G[CT][GA]CC
BbvCI 3785 CCTCAGC CCTCAGC(-5/-2)
BsaAI 643 TACGTG [CT]AC^GT[GA]
BsaBI 718 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3102 GAGACG CGTCTC(1/5)
Bsp1407I 3062 TGTACA T^GTACA
BsrDI 339 CATTGC GCAATG(2/0)
BsrGI 3062 TGTACA T^GTACA
DraII 2579 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 2579 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 3765 ATGCAT ATGCA^T
HapII 102 CCGG C^CGG
HgaI 648 GACGC GACGC(5/10)
HincII 488 GTCAAC GT[CT]^[GA]AC
HindII 488 GTCAAC GT[CT]^[GA]AC
HpaII 102 CCGG C^CGG
MamI 718 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1169 CAATTG C^AATTG
MspI 102 CCGG C^CGG
MunI 1169 CAATTG C^AATTG
NsiI 3765 ATGCAT ATGCA^T
PflFI 1063 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2579 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1265 CAGCTG CAG^CTG
SacI 1188 GAGCTC GAGCT^C
SapI 3968 GAAGAGC GCTCTTC(1/4)
SexAI 3908 ACCTGGT A^CC[AT]GGT
SmlI 853 CTTGAG C^T[CT][GA]AG
SphI 3767 GCATGC GCATG^C
SspBI 3062 TGTACA T^GTACA
Tth111I 1063 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AflIII 2364 ACATGT A^C[GA][CT]GT
3443 ACATGT
BglII 159 AGATCT A^GATCT
3874 AGATCT
BseRI 2967 CTCCTC GAGGAG(10/8)
84 GAGGAG
BspLU11I 2364 ACATGT A^CATGT
3443 ACATGT
BstXI 936 CCATATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1742 CCATTAATGTGG
DraIII 451 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1226 CACTGGGTG
FauI 5 GCGGG CCCGC(4/6)
43 GCGGG
MspA1I 1265 CAGCTG C[AC]G^C[GT]G
2620 CCGCTG
NcoI 62 CCATGG C^CATGG
2074 CCATGG
NdeI 937 CATATG CA^TATG
3249 CATATG
SpeI 1917 ACTAGT A^CTAGT
1954 ACTAGT
Tsp45I 646 GTGAC ^GT[GC]AC
2442 GTCAC
XbaI 2415 TCTAGA T^CTAGA
3877 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanII BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI