################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp718I AspEI AvaI AviII AvrII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BlnI BpuAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MluI MroI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Alw44I 2122 GTGCAC G^TGCAC ApaLI 2122 GTGCAC G^TGCAC AspI 1063 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2616 GGATCC G^GATCC BanI 518 GGCACC G^G[CT][GA]CC BbvCI 3785 CCTCAGC CCTCAGC(-5/-2) BsaAI 643 TACGTG [CT]AC^GT[GA] BsaBI 718 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3102 GAGACG CGTCTC(1/5) Bsp1407I 3062 TGTACA T^GTACA BsrDI 339 CATTGC GCAATG(2/0) BsrGI 3062 TGTACA T^GTACA DraII 2579 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO109I 2579 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT22I 3765 ATGCAT ATGCA^T HapII 102 CCGG C^CGG HgaI 648 GACGC GACGC(5/10) HincII 488 GTCAAC GT[CT]^[GA]AC HindII 488 GTCAAC GT[CT]^[GA]AC HpaII 102 CCGG C^CGG MamI 718 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1169 CAATTG C^AATTG MspI 102 CCGG C^CGG MunI 1169 CAATTG C^AATTG NsiI 3765 ATGCAT ATGCA^T PflFI 1063 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2579 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 1265 CAGCTG CAG^CTG SacI 1188 GAGCTC GAGCT^C SapI 3968 GAAGAGC GCTCTTC(1/4) SexAI 3908 ACCTGGT A^CC[AT]GGT SmlI 853 CTTGAG C^T[CT][GA]AG SphI 3767 GCATGC GCATG^C SspBI 3062 TGTACA T^GTACA Tth111I 1063 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AflIII 2364 ACATGT A^C[GA][CT]GT 3443 ACATGT BglII 159 AGATCT A^GATCT 3874 AGATCT BseRI 2967 CTCCTC GAGGAG(10/8) 84 GAGGAG BspLU11I 2364 ACATGT A^CATGT 3443 ACATGT BstXI 936 CCATATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1742 CCATTAATGTGG DraIII 451 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1226 CACTGGGTG FauI 5 GCGGG CCCGC(4/6) 43 GCGGG MspA1I 1265 CAGCTG C[AC]G^C[GT]G 2620 CCGCTG NcoI 62 CCATGG C^CATGG 2074 CCATGG NdeI 937 CATATG CA^TATG 3249 CATATG SpeI 1917 ACTAGT A^CTAGT 1954 ACTAGT Tsp45I 646 GTGAC ^GT[GC]AC 2442 GTCAC XbaI 2415 TCTAGA T^CTAGA 3877 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI