##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccIII AclI AgeI AocI
AscI Asp718I AviII BamHI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspEI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I CpoI
DraIII DrdI Eco81I EcoO65I EcoRV
EcoT22I FseI FspI KpnI KspI
MamI MluI MroI NdeI NheI
NotI NruI NsiI PacI PflMI
PinAI PmeI Psp1406I RsrII SacII
SalI SapI ScaI SfiI SgrAI
SnaBI SpeI SspBI SwaI Van91I
XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Alw44I 3404 GTGCAC G^TGCAC
Aor51HI 2211 AGCGCT AGC^GCT
ApaLI 3404 GTGCAC G^TGCAC
AseI 4515 ATTAAT AT^TAAT
AsnI 4515 ATTAAT AT^TAAT
AspI 3241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 85 CCTAGG C^CTAGG
BalI 142 TGGCCA TGG^CCA
BbeI 309 GGCGCC GGCGC^C
BbvCI 1676 GCTGAGG CCTCAGC(-5/-2)
BcgI 3317 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3843 GTATCC GTATCC(6/5)
BclI 4384 TGATCA T^GATCA
BglI 84 GCCTAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 85 CCTAGG C^CTAGG
BlpI 278 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 278 GCTGAGC GC^T[ACGT]AGC
BspDI 1186 ATCGAT AT^CGAT
BspHI 3578 TCATGA T^CATGA
BspMI 2467 GCAGGT ACCTGC(4/8)
BsrDI 4380 GCAATG GCAATG(2/0)
BssSI 9 CACGAG CACGAG(-5/-1)
BstBI 1255 TTCGAA TT^CGAA
CelII 278 GCTGAGC GC^T[ACGT]AGC
ClaI 1186 ATCGAT AT^CGAT
EagI 246 CGGCCG C^GGCCG
EclXI 246 CGGCCG C^GGCCG
Eco47III 2211 AGCGCT AGC^GCT
Eco52I 246 CGGCCG C^GGCCG
EcoNI 494 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3558 GAATTC G^AATTC
FbaI 4384 TGATCA T^GATCA
HpaI 1004 GTTAAC GTT^AAC
KasI 309 GGCGCC G^GCGCC
MluNI 142 TGGCCA TGG^CCA
MscI 142 TGGCCA TGG^CCA
NaeI 306 GCCGGC GCC^GGC
NarI 309 GGCGCC GG^CGCC
NgoMIV 306 GCCGGC G^CCGGC
NspV 1255 TTCGAA TT^CGAA
PflFI 3241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3385 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4515 ATTAAT AT^TAAT
PvuI 445 CGATCG CGAT^CG
RcaI 3578 TCATGA T^CATGA
SbfI 2587 CCTGCAGG CCTGCA^GG
SexAI 493 ACCTGGT A^CC[AT]GGT
SfoI 309 GGCGCC GGC^GCC
SfuI 1255 TTCGAA TT^CGAA
SmaI 3307 CCCGGG CCC^GGG
Sse8387I 2587 CCTGCAGG CCTGCA^GG
StuI 679 AGGCCT AGG^CCT
Tth111I 3241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3307 CCCGGG C^CCGGG
XmaI 3307 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 318 GACGTC GACGT^C
1631 GACGTC
AhdI 1978 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACATGCTGTC
Asp700I 900 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3570 GAAGGACTTC
AspEI 1978 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACATGCTGTC
BsiEI 246 CGGCCG CG[GA][CT]^CG
445 CGATCG
BspLU11I 793 ACATGT A^CATGT
4297 ACATGT
BsrBI 1692 GAGCGG CCGCTC(-3/-3)
2646 GAGCGG
BsrFI 306 GCCGGC [GA]^CCGG[CT]
3759 GCCGGT
BstXI 286 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2336 CCAAGGAAGTGG
Cfr10I 306 GCCGGC [GA]^CCGG[CT]
3759 GCCGGT
Eam1105I 1978 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACATGCTGTC
HincII 1004 GTTAAC GT[CT]^[GA]AC
3705 GTCAAC
HindII 1004 GTTAAC GT[CT]^[GA]AC
3705 GTCAAC
HindIII 1251 AAGCTT A^AGCTT
1718 AAGCTT
MfeI 2436 CAATTG C^AATTG
4241 CAATTG
MunI 2436 CAATTG C^AATTG
4241 CAATTG
PpuMI 2122 AGGACCT [GA]G^G[AT]CC[CT]
3505 GGGACCC
SacI 3204 GAGCTC GAGCT^C
4138 GAGCTC
SphI 109 GCATGC GCATG^C
4157 GCATGC
XcmI 1492 CCACACAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1516 CCAGCCTGACCATGG
XmnI 900 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3570 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvI BcnI BfaI BfrI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PleI PmaCI PmlI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoI
XhoII