################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## Acc65I AccIII AclI AgeI AocI AscI Asp718I AviII BamHI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspEI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CpoI DraIII DrdI Eco81I EcoO65I EcoRV EcoT22I FseI FspI KpnI KspI MamI MluI MroI NdeI NheI NotI NruI NsiI PacI PflMI PinAI PmeI Psp1406I RsrII SacII SalI SapI ScaI SfiI SgrAI SnaBI SpeI SspBI SwaI Van91I XbaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Alw44I 3404 GTGCAC G^TGCAC Aor51HI 2211 AGCGCT AGC^GCT ApaLI 3404 GTGCAC G^TGCAC AseI 4515 ATTAAT AT^TAAT AsnI 4515 ATTAAT AT^TAAT AspI 3241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 85 CCTAGG C^CTAGG BalI 142 TGGCCA TGG^CCA BbeI 309 GGCGCC GGCGC^C BbvCI 1676 GCTGAGG CCTCAGC(-5/-2) BcgI 3317 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3843 GTATCC GTATCC(6/5) BclI 4384 TGATCA T^GATCA BglI 84 GCCTAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 85 CCTAGG C^CTAGG BlpI 278 GCTGAGC GC^T[ACGT]AGC Bpu1102I 278 GCTGAGC GC^T[ACGT]AGC BspDI 1186 ATCGAT AT^CGAT BspHI 3578 TCATGA T^CATGA BspMI 2467 GCAGGT ACCTGC(4/8) BsrDI 4380 GCAATG GCAATG(2/0) BssSI 9 CACGAG CACGAG(-5/-1) BstBI 1255 TTCGAA TT^CGAA CelII 278 GCTGAGC GC^T[ACGT]AGC ClaI 1186 ATCGAT AT^CGAT EagI 246 CGGCCG C^GGCCG EclXI 246 CGGCCG C^GGCCG Eco47III 2211 AGCGCT AGC^GCT Eco52I 246 CGGCCG C^GGCCG EcoNI 494 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3558 GAATTC G^AATTC FbaI 4384 TGATCA T^GATCA HpaI 1004 GTTAAC GTT^AAC KasI 309 GGCGCC G^GCGCC MluNI 142 TGGCCA TGG^CCA MscI 142 TGGCCA TGG^CCA NaeI 306 GCCGGC GCC^GGC NarI 309 GGCGCC GG^CGCC NgoMIV 306 GCCGGC G^CCGGC NspV 1255 TTCGAA TT^CGAA PflFI 3241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3385 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4515 ATTAAT AT^TAAT PvuI 445 CGATCG CGAT^CG RcaI 3578 TCATGA T^CATGA SbfI 2587 CCTGCAGG CCTGCA^GG SexAI 493 ACCTGGT A^CC[AT]GGT SfoI 309 GGCGCC GGC^GCC SfuI 1255 TTCGAA TT^CGAA SmaI 3307 CCCGGG CCC^GGG Sse8387I 2587 CCTGCAGG CCTGCA^GG StuI 679 AGGCCT AGG^CCT Tth111I 3241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 3307 CCCGGG C^CCGGG XmaI 3307 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 318 GACGTC GACGT^C 1631 GACGTC AhdI 1978 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2960 GACATGCTGTC Asp700I 900 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3570 GAAGGACTTC AspEI 1978 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2960 GACATGCTGTC BsiEI 246 CGGCCG CG[GA][CT]^CG 445 CGATCG BspLU11I 793 ACATGT A^CATGT 4297 ACATGT BsrBI 1692 GAGCGG CCGCTC(-3/-3) 2646 GAGCGG BsrFI 306 GCCGGC [GA]^CCGG[CT] 3759 GCCGGT BstXI 286 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2336 CCAAGGAAGTGG Cfr10I 306 GCCGGC [GA]^CCGG[CT] 3759 GCCGGT Eam1105I 1978 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2960 GACATGCTGTC HincII 1004 GTTAAC GT[CT]^[GA]AC 3705 GTCAAC HindII 1004 GTTAAC GT[CT]^[GA]AC 3705 GTCAAC HindIII 1251 AAGCTT A^AGCTT 1718 AAGCTT MfeI 2436 CAATTG C^AATTG 4241 CAATTG MunI 2436 CAATTG C^AATTG 4241 CAATTG PpuMI 2122 AGGACCT [GA]G^G[AT]CC[CT] 3505 GGGACCC SacI 3204 GAGCTC GAGCT^C 4138 GAGCTC SphI 109 GCATGC GCATG^C 4157 GCATGC XcmI 1492 CCACACAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1516 CCAGCCTGACCATGG XmnI 900 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3570 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BcnI BfaI BfrI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PleI PmaCI PmlI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoI XhoII