##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI AscI AspEI
AviII BamHI BbeI BciVI BclI
BglI BglII BlpI Bpu1102I BsaHI
BsaWI BseAI BsiWI BspEI BssHII
BssSI BstBI BstEII BstPI BstUI
CelII CpoI DraIII Eam1105I Eco47III
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII Hin1I KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SacI
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 3187 GGTACC G^GTACC
AflII 3934 CTTAAG C^TTAAG
Alw44I 1116 GTGCAC G^TGCAC
ApaLI 1116 GTGCAC G^TGCAC
AseI 1671 ATTAAT AT^TAAT
AsnI 1671 ATTAAT AT^TAAT
Asp718I 3187 GGTACC G^GTACC
AvrII 3376 CCTAGG C^CTAGG
BanI 3187 GGTACC G^G[CT][GA]CC
BbrPI 1976 CACGTG CAC^GTG
BcgI 4205 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 635 CCCGG CC^[GC]GG
BfrI 3934 CTTAAG C^TTAAG
BlnI 3376 CCTAGG C^CTAGG
BsaAI 1976 CACGTG [CT]AC^GT[GA]
BsaBI 3366 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 647 CGGCCG CG[GA][CT]^CG
BsmBI 701 CGTCTC CGTCTC(1/5)
BsmI 2289 GAATGC GAATGC(1/-1)
Bsp1407I 3833 TGTACA T^GTACA
BspDI 558 ATCGAT AT^CGAT
BspHI 1295 TCATGA T^CATGA
BsrBI 650 CCGCTC CCGCTC(-3/-3)
BsrGI 3833 TGTACA T^GTACA
Bst1107I 1145 GTATAC GTA^TAC
BstAPI 1962 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 584 AGATCC [GA]^GATC[CT]
BstZ17I 1145 GTATAC GTA^TAC
CfoI 666 GCGC GCG^C
ClaI 558 ATCGAT AT^CGAT
DrdI 785 GACATGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3095 CCATGG C^C[GA][CT]GG
EagI 647 CGGCCG C^GGCCG
EclXI 647 CGGCCG C^GGCCG
Eco52I 647 CGGCCG C^GGCCG
EcoNI 453 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1299 GAATTC G^AATTC
HgaI 1494 GCGTC GACGC(5/10)
HhaI 666 GCGC GCG^C
HinP1I 666 GCGC G^CGC
KpnI 3187 GGTACC GGTAC^C
MamI 3366 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 584 AGATCC [GA]^GATC[CT]
NciI 635 CCCGG CC^[GC]GG
NcoI 3095 CCATGG C^CATGG
NotI 646 GCGGCCGC GC^GGCCGC
PmaCI 1976 CACGTG CAC^GTG
PmlI 1976 CACGTG CAC^GTG
PshBI 1671 ATTAAT AT^TAAT
RcaI 1295 TCATGA T^CATGA
SalI 2811 GTCGAC G^TCGAC
ScaI 3158 AGTACT AGT^ACT
SspBI 3833 TGTACA T^GTACA
SspI 2300 AATATT AAT^ATT
StuI 3453 AGGCCT AGG^CCT
XbaI 1030 TCTAGA T^CTAGA
XcmI 3708 CCAGGCCAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 584 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 4036 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
4164 CAGGTACTG
AocI 1016 CCTGAGG CC^T[ACGT]AGG
3449 CCTGAGG
ApaI 2281 GGGCCC GGGCC^C
2756 GGGCCC
AspI 3523 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4019 GACTTTGTC
AvaI 468 CTCGGG C^[CT]CG[GA]G
3128 CTCGGG
BalI 2900 TGGCCA TGG^CCA
3809 TGGCCA
BbsI 27 GAAGAC GAAGAC(2/6)
2537 GAAGAC
BbvCI 1541 CCTCAGC CCTCAGC(-5/-2)
3145 CCTCAGC
BpuAI 27 GAAGAC GAAGAC(2/6)
2537 GAAGAC
BsaI 325 GAGACC GGTCTC(1/5)
714 GAGACC
BsgI 752 GTGCAG GTGCAG(16/14)
936 GTGCAG
BsoBI 468 CTCGGG C^[CT]CG[GA]G
3128 CTCGGG
BspMI 1536 ACCTGC ACCTGC(4/8)
3754 ACCTGC
BsrDI 1230 CATTGC GCAATG(2/0)
2799 CATTGC
BstXI 1882 CCACAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2211 CCATTCAGCTGG
Bsu36I 1016 CCTGAGG CC^T[ACGT]AGG
3449 CCTGAGG
Eco81I 1016 CCTGAGG CC^T[ACGT]AGG
3449 CCTGAGG
EcoT22I 1969 ATGCAT ATGCA^T
2489 ATGCAT
HindIII 773 AAGCTT A^AGCTT
1552 AAGCTT
HpaI 1100 GTTAAC GTT^AAC
1240 GTTAAC
MluNI 2900 TGGCCA TGG^CCA
3809 TGGCCA
MscI 2900 TGGCCA TGG^CCA
3809 TGGCCA
NsiI 1969 ATGCAT ATGCA^T
2489 ATGCAT
PflFI 3523 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4019 GACTTTGTC
PstI 1475 CTGCAG CTGCA^G
1957 CTGCAG
SapI 1489 GAAGAGC GCTCTTC(1/4)
4200 GAAGAGC
TaqI 559 TCGA T^CGA
2812 TCGA
Tth111I 3523 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4019 GACTTTGTC
TthHB8I 559 TCGA T^CGA
2812 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XmnI