################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI AhdI Aor51HI AscI AspEI AviII BamHI BbeI BciVI BclI BglI BglII BlpI Bpu1102I BsaHI BsaWI BseAI BsiWI BspEI BssHII BssSI BstBI BstEII BstPI BstUI CelII CpoI DraIII Eam1105I Eco47III EcoO65I EcoRV FauI FbaI FseI FspI HaeII Hin1I KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacI SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 3187 GGTACC G^GTACC AflII 3934 CTTAAG C^TTAAG Alw44I 1116 GTGCAC G^TGCAC ApaLI 1116 GTGCAC G^TGCAC AseI 1671 ATTAAT AT^TAAT AsnI 1671 ATTAAT AT^TAAT Asp718I 3187 GGTACC G^GTACC AvrII 3376 CCTAGG C^CTAGG BanI 3187 GGTACC G^G[CT][GA]CC BbrPI 1976 CACGTG CAC^GTG BcgI 4205 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 635 CCCGG CC^[GC]GG BfrI 3934 CTTAAG C^TTAAG BlnI 3376 CCTAGG C^CTAGG BsaAI 1976 CACGTG [CT]AC^GT[GA] BsaBI 3366 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 647 CGGCCG CG[GA][CT]^CG BsmBI 701 CGTCTC CGTCTC(1/5) BsmI 2289 GAATGC GAATGC(1/-1) Bsp1407I 3833 TGTACA T^GTACA BspDI 558 ATCGAT AT^CGAT BspHI 1295 TCATGA T^CATGA BsrBI 650 CCGCTC CCGCTC(-3/-3) BsrGI 3833 TGTACA T^GTACA Bst1107I 1145 GTATAC GTA^TAC BstAPI 1962 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 584 AGATCC [GA]^GATC[CT] BstZ17I 1145 GTATAC GTA^TAC CfoI 666 GCGC GCG^C ClaI 558 ATCGAT AT^CGAT DrdI 785 GACATGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 3095 CCATGG C^C[GA][CT]GG EagI 647 CGGCCG C^GGCCG EclXI 647 CGGCCG C^GGCCG Eco52I 647 CGGCCG C^GGCCG EcoNI 453 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1299 GAATTC G^AATTC HgaI 1494 GCGTC GACGC(5/10) HhaI 666 GCGC GCG^C HinP1I 666 GCGC G^CGC KpnI 3187 GGTACC GGTAC^C MamI 3366 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 584 AGATCC [GA]^GATC[CT] NciI 635 CCCGG CC^[GC]GG NcoI 3095 CCATGG C^CATGG NotI 646 GCGGCCGC GC^GGCCGC PmaCI 1976 CACGTG CAC^GTG PmlI 1976 CACGTG CAC^GTG PshBI 1671 ATTAAT AT^TAAT RcaI 1295 TCATGA T^CATGA SalI 2811 GTCGAC G^TCGAC ScaI 3158 AGTACT AGT^ACT SspBI 3833 TGTACA T^GTACA SspI 2300 AATATT AAT^ATT StuI 3453 AGGCCT AGG^CCT XbaI 1030 TCTAGA T^CTAGA XcmI 3708 CCAGGCCAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 584 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 4036 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 4164 CAGGTACTG AocI 1016 CCTGAGG CC^T[ACGT]AGG 3449 CCTGAGG ApaI 2281 GGGCCC GGGCC^C 2756 GGGCCC AspI 3523 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4019 GACTTTGTC AvaI 468 CTCGGG C^[CT]CG[GA]G 3128 CTCGGG BalI 2900 TGGCCA TGG^CCA 3809 TGGCCA BbsI 27 GAAGAC GAAGAC(2/6) 2537 GAAGAC BbvCI 1541 CCTCAGC CCTCAGC(-5/-2) 3145 CCTCAGC BpuAI 27 GAAGAC GAAGAC(2/6) 2537 GAAGAC BsaI 325 GAGACC GGTCTC(1/5) 714 GAGACC BsgI 752 GTGCAG GTGCAG(16/14) 936 GTGCAG BsoBI 468 CTCGGG C^[CT]CG[GA]G 3128 CTCGGG BspMI 1536 ACCTGC ACCTGC(4/8) 3754 ACCTGC BsrDI 1230 CATTGC GCAATG(2/0) 2799 CATTGC BstXI 1882 CCACAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2211 CCATTCAGCTGG Bsu36I 1016 CCTGAGG CC^T[ACGT]AGG 3449 CCTGAGG Eco81I 1016 CCTGAGG CC^T[ACGT]AGG 3449 CCTGAGG EcoT22I 1969 ATGCAT ATGCA^T 2489 ATGCAT HindIII 773 AAGCTT A^AGCTT 1552 AAGCTT HpaI 1100 GTTAAC GTT^AAC 1240 GTTAAC MluNI 2900 TGGCCA TGG^CCA 3809 TGGCCA MscI 2900 TGGCCA TGG^CCA 3809 TGGCCA NsiI 1969 ATGCAT ATGCA^T 2489 ATGCAT PflFI 3523 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4019 GACTTTGTC PstI 1475 CTGCAG CTGCA^G 1957 CTGCAG SapI 1489 GAAGAGC GCTCTTC(1/4) 4200 GAAGAGC TaqI 559 TCGA T^CGA 2812 TCGA Tth111I 3523 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4019 GACTTTGTC TthHB8I 559 TCGA T^CGA 2812 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XmnI