##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AvaI AviII AvrII BbrPI
BbvCI BcgI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BsrBI
BsrGI BssHII BstBI BstEII BstPI
BstUI BstXI CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FauI FseI FspI HgaI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 29 GACGTC GACGT^C
AccI 2079 GTATAC GT^[AC][GT]AC
AccIII 25 TCCGGA T^CCGGA
Alw44I 3021 GTGCAC G^TGCAC
AlwNI 2492 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2929 CCTTAGG CC^T[ACGT]AGG
ApaLI 3021 GTGCAC G^TGCAC
BalI 3001 TGGCCA TGG^CCA
BamHI 891 GGATCC G^GATCC
BbeI 768 GGCGCC GGCGC^C
BciVI 1356 GGATAC GTATCC(6/5)
BclI 1016 TGATCA T^GATCA
BcnI 2831 CCGGG CC^[GC]GG
BseAI 25 TCCGGA T^CCGGA
BsgI 830 CTGCAC GTGCAG(16/14)
BsmI 453 GAATGC GAATGC(1/-1)
BspEI 25 TCCGGA T^CCGGA
BspMI 2911 ACCTGC ACCTGC(4/8)
BsrFI 1223 ACCGGC [GA]^CCGG[CT]
Bst1107I 2079 GTATAC GTA^TAC
BstAPI 457 GCACTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2079 GTATAC GTA^TAC
Bsu36I 2929 CCTTAGG CC^T[ACGT]AGG
CfoI 769 GCGC GCG^C
Cfr10I 1223 ACCGGC [GA]^CCGG[CT]
DraII 126 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 238 CCATGG C^C[GA][CT]GG
EaeI 3001 TGGCCA [CT]^GGCC[GA]
Eco81I 2929 CCTTAGG CC^T[ACGT]AGG
EcoO109I 126 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 306 GATATC GAT^ATC
FbaI 1016 TGATCA T^GATCA
HaeII 768 GGCGCC [GA]GCGC^[CT]
HhaI 769 GCGC GCG^C
HinP1I 769 GCGC G^CGC
HpaI 2532 GTTAAC GTT^AAC
KasI 768 GGCGCC G^GCGCC
MluNI 3001 TGGCCA TGG^CCA
MroI 25 TCCGGA T^CCGGA
MscI 3001 TGGCCA TGG^CCA
MspA1I 38 CAGCGG C[AC]G^C[GT]G
NarI 768 GGCGCC GG^CGCC
NciI 2831 CCGGG CC^[GC]GG
NcoI 238 CCATGG C^CATGG
PflMI 131 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 126 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 636 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 746 GCTCTTC GCTCTTC(1/4)
SfoI 768 GGCGCC GGC^GCC
SpeI 2868 ACTAGT A^CTAGT
SwaI 3262 ATTTAAAT ATTT^AAAT
Van91I 131 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 29 GACGTC G[GA]^CG[CT]C
768 GGCGCC
AflII 1977 CTTAAG C^TTAAG
3068 CTTAAG
AflIII 2000 ACATGT A^C[GA][CT]GT
2536 ACATGT
AseI 3614 ATTAAT AT^TAAT
3758 ATTAAT
AsnI 3614 ATTAAT AT^TAAT
3758 ATTAAT
AspI 53 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
519 GACTGTGTC
AvaII 127 GGACC G^G[AT]CC
3803 GGACC
BanI 768 GGCGCC G^G[CT][GA]CC
2581 GGCACC
BfrI 1977 CTTAAG C^TTAAG
3068 CTTAAG
BsaHI 29 GACGTC G[GA]^CG[CT]C
768 GGCGCC
BsaWI 25 TCCGGA [AT]^CCGG[AT]
565 TCCGGT
BspLU11I 2000 ACATGT A^CATGT
2536 ACATGT
BsrDI 3724 CATTGC GCAATG(2/0)
869 GCAATG
BssSI 2171 CACGAG CACGAG(-5/-1)
3220 CACGAG
Cfr13I 127 GGACC G^G[ACGT]CC
3803 GGACC
Hin1I 29 GACGTC G[GA]^CG[CT]C
768 GGCGCC
HincII 428 GTCAAC GT[CT]^[GA]AC
2532 GTTAAC
HindII 428 GTCAAC GT[CT]^[GA]AC
2532 GTTAAC
MaeII 30 ACGT A^CGT
2265 ACGT
MslI 2190 CACTCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3651 CATCTTAGTG
PflFI 53 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
519 GACTGTGTC
PshBI 3614 ATTAAT AT^TAAT
3758 ATTAAT
PstI 35 CTGCAG CTGCA^G
2422 CTGCAG
Sau96I 127 GGACC G^G[ACGT]CC
3803 GGACC
SexAI 1368 ACCTGGT A^CC[AT]GGT
2381 ACCTGGT
Tth111I 53 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
519 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
SacI Sau3AI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII