################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccII AclI AgeI AhdI Aor51HI ApaI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BbrPI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BsrBI BsrGI BssHII BstBI BstEII BstPI BstUI BstXI CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HgaI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 29 GACGTC GACGT^C AccI 2079 GTATAC GT^[AC][GT]AC AccIII 25 TCCGGA T^CCGGA Alw44I 3021 GTGCAC G^TGCAC AlwNI 2492 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2929 CCTTAGG CC^T[ACGT]AGG ApaLI 3021 GTGCAC G^TGCAC BalI 3001 TGGCCA TGG^CCA BamHI 891 GGATCC G^GATCC BbeI 768 GGCGCC GGCGC^C BciVI 1356 GGATAC GTATCC(6/5) BclI 1016 TGATCA T^GATCA BcnI 2831 CCGGG CC^[GC]GG BseAI 25 TCCGGA T^CCGGA BsgI 830 CTGCAC GTGCAG(16/14) BsmI 453 GAATGC GAATGC(1/-1) BspEI 25 TCCGGA T^CCGGA BspMI 2911 ACCTGC ACCTGC(4/8) BsrFI 1223 ACCGGC [GA]^CCGG[CT] Bst1107I 2079 GTATAC GTA^TAC BstAPI 457 GCACTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2079 GTATAC GTA^TAC Bsu36I 2929 CCTTAGG CC^T[ACGT]AGG CfoI 769 GCGC GCG^C Cfr10I 1223 ACCGGC [GA]^CCGG[CT] DraII 126 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 238 CCATGG C^C[GA][CT]GG EaeI 3001 TGGCCA [CT]^GGCC[GA] Eco81I 2929 CCTTAGG CC^T[ACGT]AGG EcoO109I 126 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 306 GATATC GAT^ATC FbaI 1016 TGATCA T^GATCA HaeII 768 GGCGCC [GA]GCGC^[CT] HhaI 769 GCGC GCG^C HinP1I 769 GCGC G^CGC HpaI 2532 GTTAAC GTT^AAC KasI 768 GGCGCC G^GCGCC MluNI 3001 TGGCCA TGG^CCA MroI 25 TCCGGA T^CCGGA MscI 3001 TGGCCA TGG^CCA MspA1I 38 CAGCGG C[AC]G^C[GT]G NarI 768 GGCGCC GG^CGCC NciI 2831 CCGGG CC^[GC]GG NcoI 238 CCATGG C^CATGG PflMI 131 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 126 AGGACCC [GA]G^G[AT]CC[CT] PshAI 636 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 746 GCTCTTC GCTCTTC(1/4) SfoI 768 GGCGCC GGC^GCC SpeI 2868 ACTAGT A^CTAGT SwaI 3262 ATTTAAAT ATTT^AAAT Van91I 131 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 29 GACGTC G[GA]^CG[CT]C 768 GGCGCC AflII 1977 CTTAAG C^TTAAG 3068 CTTAAG AflIII 2000 ACATGT A^C[GA][CT]GT 2536 ACATGT AseI 3614 ATTAAT AT^TAAT 3758 ATTAAT AsnI 3614 ATTAAT AT^TAAT 3758 ATTAAT AspI 53 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 519 GACTGTGTC AvaII 127 GGACC G^G[AT]CC 3803 GGACC BanI 768 GGCGCC G^G[CT][GA]CC 2581 GGCACC BfrI 1977 CTTAAG C^TTAAG 3068 CTTAAG BsaHI 29 GACGTC G[GA]^CG[CT]C 768 GGCGCC BsaWI 25 TCCGGA [AT]^CCGG[AT] 565 TCCGGT BspLU11I 2000 ACATGT A^CATGT 2536 ACATGT BsrDI 3724 CATTGC GCAATG(2/0) 869 GCAATG BssSI 2171 CACGAG CACGAG(-5/-1) 3220 CACGAG Cfr13I 127 GGACC G^G[ACGT]CC 3803 GGACC Hin1I 29 GACGTC G[GA]^CG[CT]C 768 GGCGCC HincII 428 GTCAAC GT[CT]^[GA]AC 2532 GTTAAC HindII 428 GTCAAC GT[CT]^[GA]AC 2532 GTTAAC MaeII 30 ACGT A^CGT 2265 ACGT MslI 2190 CACTCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3651 CATCTTAGTG PflFI 53 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 519 GACTGTGTC PshBI 3614 ATTAAT AT^TAAT 3758 ATTAAT PstI 35 CTGCAG CTGCA^G 2422 CTGCAG Sau96I 127 GGACC G^G[ACGT]CC 3803 GGACC SexAI 1368 ACCTGGT A^CC[AT]GGT 2381 ACCTGGT Tth111I 53 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 519 GACTGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI RcaI RsaI SacI Sau3AI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII