##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AccIII AclI AgeI AscI
AseI AsnI Asp700I AspI AviII
BciVI BsaBI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
MamI MfeI MluI MroI MunI
NdeI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SfuI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 939 GACGTC GACGT^C
Acc65I 4196 GGTACC G^GTACC
AcsI 405 AAATTC [GA]^AATT[CT]
AflII 1887 CTTAAG C^TTAAG
Aor51HI 1170 AGCGCT AGC^GCT
ApoI 405 AAATTC [GA]^AATT[CT]
Asp718I 4196 GGTACC G^GTACC
AvrII 3460 CCTAGG C^CTAGG
BamHI 1419 GGATCC G^GATCC
BclI 3839 TGATCA T^GATCA
BfrI 1887 CTTAAG C^TTAAG
BglII 454 AGATCT A^GATCT
BlnI 3460 CCTAGG C^CTAGG
BsaWI 4594 TCCGGT [AT]^CCGG[AT]
BsmBI 309 GAGACG CGTCTC(1/5)
BsmI 3173 GAATGC GAATGC(1/-1)
Bsp1407I 1939 TGTACA T^GTACA
BspHI 1426 TCATGA T^CATGA
BspLU11I 1474 ACATGT A^CATGT
BsrGI 1939 TGTACA T^GTACA
BssSI 3428 CTCGTG CACGAG(-5/-1)
BstEII 4392 GGTTACC G^GT[ACGT]ACC
BstPI 4392 GGTTACC G^GT[ACGT]ACC
DraI 4741 TTTAAA TTT^AAA
DraIII 2022 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3436 GACTCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1170 AGCGCT AGC^GCT
EcoO65I 4392 GGTTACC G^GT[ACGT]ACC
FbaI 3839 TGATCA T^GATCA
KpnI 4196 GGTACC GGTAC^C
NheI 4362 GCTAGC G^CTAGC
PshAI 3105 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1426 TCATGA T^CATGA
SapI 1747 GCTCTTC GCTCTTC(1/4)
SgrAI 920 CACCGGCG C[GA]^CCGG[CT]G
SphI 4413 GCATGC GCATG^C
SspBI 1939 TGTACA T^GTACA
XbaI 750 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 393 GTGCAC G^TGCAC
4227 GTGCAC
AlwNI 1768 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3374 CAGGTCCTG
AocI 2015 CCTGAGG CC^T[ACGT]AGG
2711 CCTCAGG
ApaLI 393 GTGCAC G^TGCAC
4227 GTGCAC
BbvCI 4108 CCTCAGC CCTCAGC(-5/-2)
519 GCTGAGG
BsaI 276 GAGACC GGTCTC(1/5)
4504 GAGACC
BsgI 3680 CTGCAC GTGCAG(16/14)
2078 GTGCAG
BsrBI 60 CCGCTC CCGCTC(-3/-3)
3602 GAGCGG
Bsu36I 2015 CCTGAGG CC^T[ACGT]AGG
2711 CCTCAGG
Eco81I 2015 CCTGAGG CC^T[ACGT]AGG
2711 CCTCAGG
EcoNI 515 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3688 CCTTACAAAGG
HincII 1497 GTCAAC GT[CT]^[GA]AC
4691 GTTGAC
HindII 1497 GTCAAC GT[CT]^[GA]AC
4691 GTTGAC
NaeI 185 GCCGGC GCC^GGC
1095 GCCGGC
NgoMIV 185 GCCGGC G^CCGGC
1095 GCCGGC
NotI 84 GCGGCCGC GC^GGCCGC
142 GCGGCCGC
PstI 1342 CTGCAG CTGCA^G
1482 CTGCAG
SacI 653 GAGCTC GAGCT^C
3897 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII