##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BbsI        BlnI        BpuAI       
BsiEI       BsiWI       BspLU11I    BsrBI       BssHII      
Bst1107I    BstBI       BstZ17I     CpoI        EagI        
EclXI       Eco52I      FseI        FspI        HindIII     
KpnI        KspI        MfeI        MluI        MunI        
NheI        NotI        NruI        NspV        PaeR7I      
PflFI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspI        SwaI        
Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII       2929  TCCGGA               T^CCGGA
AflII        2837  CTTAAG               C^TTAAG
AgeI          866  ACCGGT               A^CCGGT
Alw44I       3753  GTGCAC               G^TGCAC
AocI         4414  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2757  AGCGCT               AGC^GCT
ApaLI        3753  GTGCAC               G^TGCAC
BbeI         2898  GGCGCC               GGCGC^C
BbvCI        3570  GCTGAGG              CCTCAGC(-5/-2)
BcgI          766  GCAGCCACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3782  GTATCC               GTATCC(6/5)
BclI         4451  TGATCA               T^GATCA
BfrI         2837  CTTAAG               C^TTAAG
BlpI         2266  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2266  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2187  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2929  TCCGGA               T^CCGGA
BsmBI        4150  CGTCTC               CGTCTC(1/5)
BsmI         2484  GCATTC               GAATGC(1/-1)
Bsp1407I     1301  TGTACA               T^GTACA
BspDI        2709  ATCGAT               AT^CGAT
BspEI        2929  TCCGGA               T^CCGGA
BsrGI        1301  TGTACA               T^GTACA
BssSI        3527  CTCGTG               CACGAG(-5/-1)
BstEII       4339  GGTGACC              G^GT[ACGT]ACC
BstPI        4339  GGTGACC              G^GT[ACGT]ACC
Bsu36I       4414  CCTGAGG              CC^T[ACGT]AGG
CelII        2266  GCTGAGC              GC^T[ACGT]AGC
ClaI         2709  ATCGAT               AT^CGAT
DrdI          981  GACCTGCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2757  AGCGCT               AGC^GCT
Eco81I       4414  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      4339  GGTGACC              G^GT[ACGT]ACC
EcoRI        3095  GAATTC               G^AATTC
EcoRV        1518  GATATC               GAT^ATC
EcoT22I      3046  ATGCAT               ATGCA^T
FbaI         4451  TGATCA               T^GATCA
HpaI         3841  GTTAAC               GTT^AAC
KasI         2898  GGCGCC               G^GCGCC
MamI         2187  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2929  TCCGGA               T^CCGGA
NaeI         2416  GCCGGC               GCC^GGC
NarI         2898  GGCGCC               GG^CGCC
NcoI         3084  CCATGG               C^CATGG
NgoMIV       2416  GCCGGC               G^CCGGC
NsiI         3046  ATGCAT               ATGCA^T
PacI         4309  TTAATTAA             TTAAT^TAA
PinAI         866  ACCGGT               A^CCGGT
SfoI         2898  GGCGCC               GGC^GCC
SmaI           65  CCCGGG               CCC^GGG
SspBI        1301  TGTACA               T^GTACA
StuI         1531  AGGCCT               AGG^CCT
XcmI         2754  CCAAGCGCTTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          65  CCCGGG               C^CCGGG
XmaI           65  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI          509  GTAGAC               GT^[AC][GT]AC
             2092  GTCTAC              
AcyI         2570  GGCGTC               G[GA]^CG[CT]C
             2898  GGCGCC              
AhdI         1147  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3660  GACTGCAAGTC         
AspEI        1147  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3660  GACTGCAAGTC         
AspHI        1312  GTGCTC               G[AT]GC[AT]^C
             3753  GTGCAC              
BalI          597  TGGCCA               TGG^CCA
             1254  TGGCCA              
BamHI        2539  GGATCC               G^GATCC
             3984  GGATCC              
BbrPI           8  CACGTG               CAC^GTG
             1009  CACGTG              
BglII         365  AGATCT               A^GATCT
             4193  AGATCT              
BsaAI           8  CACGTG               [CT]AC^GT[GA]
             1009  CACGTG              
BsaHI        2570  GGCGTC               G[GA]^CG[CT]C
             2898  GGCGCC              
BsaWI         866  ACCGGT               [AT]^CCGG[AT]
             2929  TCCGGA              
BsiHKAI      1312  GTGCTC               G[AT]GC[AT]^C
             3753  GTGCAC              
BspHI        1285  TCATGA               T^CATGA
             4522  TCATGA              
BsrFI         866  ACCGGT               [GA]^CCGG[CT]
             2416  GCCGGC              
BstAPI       3341  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4388  GCAGCCTCTGC         
BstXI        1738  CCATGCCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3494  CCATTGGGTTGG        
Cfr10I        866  ACCGGT               [GA]^CCGG[CT]
             2416  GCCGGC              
DraIII          8  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
               26  CACCGCGTG           
DsaI          348  CCACGG               C^C[GA][CT]GG
             3084  CCATGG              
Eam1105I     1147  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3660  GACTGCAAGTC         
EarI         1726  CTCTTC               CTCTTC(1/4)
             4085  CTCTTC              
HaeII        2757  AGCGCT               [GA]GCGC^[CT]
             2898  GGCGCC              
Hin1I        2570  GGCGTC               G[GA]^CG[CT]C
             2898  GGCGCC              
Ksp632I      1726  CTCTTC               CTCTTC(1/4)
             4085  CTCTTC              
MluNI         597  TGGCCA               TGG^CCA
             1254  TGGCCA              
MscI          597  TGGCCA               TGG^CCA
             1254  TGGCCA              
NdeI          930  CATATG               CA^TATG
             4003  CATATG              
PmaCI           8  CACGTG               CAC^GTG
             1009  CACGTG              
PmlI            8  CACGTG               CAC^GTG
             1009  CACGTG              
RcaI         1285  TCATGA               T^CATGA
             4522  TCATGA              
SbfI          299  CCTGCAGG             CCTGCA^GG
             2653  CCTGCAGG            
SexAI         567  ACCTGGT              A^CC[AT]GGT
             4343  ACCAGGT             
Sse8387I      299  CCTGCAGG             CCTGCA^GG
             2653  CCTGCAGG            
XbaI          538  TCTAGA               T^CTAGA
             4895  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII