##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AflIII AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BbsI BlnI BpuAI
BsiEI BsiWI BspLU11I BsrBI BssHII
Bst1107I BstBI BstZ17I CpoI EagI
EclXI Eco52I FseI FspI HindIII
KpnI KspI MfeI MluI MunI
NheI NotI NruI NspV PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 2929 TCCGGA T^CCGGA
AflII 2837 CTTAAG C^TTAAG
AgeI 866 ACCGGT A^CCGGT
Alw44I 3753 GTGCAC G^TGCAC
AocI 4414 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2757 AGCGCT AGC^GCT
ApaLI 3753 GTGCAC G^TGCAC
BbeI 2898 GGCGCC GGCGC^C
BbvCI 3570 GCTGAGG CCTCAGC(-5/-2)
BcgI 766 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3782 GTATCC GTATCC(6/5)
BclI 4451 TGATCA T^GATCA
BfrI 2837 CTTAAG C^TTAAG
BlpI 2266 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2266 GCTGAGC GC^T[ACGT]AGC
BsaBI 2187 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2929 TCCGGA T^CCGGA
BsmBI 4150 CGTCTC CGTCTC(1/5)
BsmI 2484 GCATTC GAATGC(1/-1)
Bsp1407I 1301 TGTACA T^GTACA
BspDI 2709 ATCGAT AT^CGAT
BspEI 2929 TCCGGA T^CCGGA
BsrGI 1301 TGTACA T^GTACA
BssSI 3527 CTCGTG CACGAG(-5/-1)
BstEII 4339 GGTGACC G^GT[ACGT]ACC
BstPI 4339 GGTGACC G^GT[ACGT]ACC
Bsu36I 4414 CCTGAGG CC^T[ACGT]AGG
CelII 2266 GCTGAGC GC^T[ACGT]AGC
ClaI 2709 ATCGAT AT^CGAT
DrdI 981 GACCTGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2757 AGCGCT AGC^GCT
Eco81I 4414 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4339 GGTGACC G^GT[ACGT]ACC
EcoRI 3095 GAATTC G^AATTC
EcoRV 1518 GATATC GAT^ATC
EcoT22I 3046 ATGCAT ATGCA^T
FbaI 4451 TGATCA T^GATCA
HpaI 3841 GTTAAC GTT^AAC
KasI 2898 GGCGCC G^GCGCC
MamI 2187 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2929 TCCGGA T^CCGGA
NaeI 2416 GCCGGC GCC^GGC
NarI 2898 GGCGCC GG^CGCC
NcoI 3084 CCATGG C^CATGG
NgoMIV 2416 GCCGGC G^CCGGC
NsiI 3046 ATGCAT ATGCA^T
PacI 4309 TTAATTAA TTAAT^TAA
PinAI 866 ACCGGT A^CCGGT
SfoI 2898 GGCGCC GGC^GCC
SmaI 65 CCCGGG CCC^GGG
SspBI 1301 TGTACA T^GTACA
StuI 1531 AGGCCT AGG^CCT
XcmI 2754 CCAAGCGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 509 GTAGAC GT^[AC][GT]AC
2092 GTCTAC
AcyI 2570 GGCGTC G[GA]^CG[CT]C
2898 GGCGCC
AhdI 1147 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3660 GACTGCAAGTC
AspEI 1147 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3660 GACTGCAAGTC
AspHI 1312 GTGCTC G[AT]GC[AT]^C
3753 GTGCAC
BalI 597 TGGCCA TGG^CCA
1254 TGGCCA
BamHI 2539 GGATCC G^GATCC
3984 GGATCC
BbrPI 8 CACGTG CAC^GTG
1009 CACGTG
BglII 365 AGATCT A^GATCT
4193 AGATCT
BsaAI 8 CACGTG [CT]AC^GT[GA]
1009 CACGTG
BsaHI 2570 GGCGTC G[GA]^CG[CT]C
2898 GGCGCC
BsaWI 866 ACCGGT [AT]^CCGG[AT]
2929 TCCGGA
BsiHKAI 1312 GTGCTC G[AT]GC[AT]^C
3753 GTGCAC
BspHI 1285 TCATGA T^CATGA
4522 TCATGA
BsrFI 866 ACCGGT [GA]^CCGG[CT]
2416 GCCGGC
BstAPI 3341 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4388 GCAGCCTCTGC
BstXI 1738 CCATGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3494 CCATTGGGTTGG
Cfr10I 866 ACCGGT [GA]^CCGG[CT]
2416 GCCGGC
DraIII 8 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
26 CACCGCGTG
DsaI 348 CCACGG C^C[GA][CT]GG
3084 CCATGG
Eam1105I 1147 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3660 GACTGCAAGTC
EarI 1726 CTCTTC CTCTTC(1/4)
4085 CTCTTC
HaeII 2757 AGCGCT [GA]GCGC^[CT]
2898 GGCGCC
Hin1I 2570 GGCGTC G[GA]^CG[CT]C
2898 GGCGCC
Ksp632I 1726 CTCTTC CTCTTC(1/4)
4085 CTCTTC
MluNI 597 TGGCCA TGG^CCA
1254 TGGCCA
MscI 597 TGGCCA TGG^CCA
1254 TGGCCA
NdeI 930 CATATG CA^TATG
4003 CATATG
PmaCI 8 CACGTG CAC^GTG
1009 CACGTG
PmlI 8 CACGTG CAC^GTG
1009 CACGTG
RcaI 1285 TCATGA T^CATGA
4522 TCATGA
SbfI 299 CCTGCAGG CCTGCA^GG
2653 CCTGCAGG
SexAI 567 ACCTGGT A^CC[AT]GGT
4343 ACCAGGT
Sse8387I 299 CCTGCAGG CCTGCA^GG
2653 CCTGCAGG
XbaI 538 TCTAGA T^CTAGA
4895 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII