################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII Acc65I AclI AflIII AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbsI BlnI BpuAI BsiEI BsiWI BspLU11I BsrBI BssHII Bst1107I BstBI BstZ17I CpoI EagI EclXI Eco52I FseI FspI HindIII KpnI KspI MfeI MluI MunI NheI NotI NruI NspV PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 2929 TCCGGA T^CCGGA AflII 2837 CTTAAG C^TTAAG AgeI 866 ACCGGT A^CCGGT Alw44I 3753 GTGCAC G^TGCAC AocI 4414 CCTGAGG CC^T[ACGT]AGG Aor51HI 2757 AGCGCT AGC^GCT ApaLI 3753 GTGCAC G^TGCAC BbeI 2898 GGCGCC GGCGC^C BbvCI 3570 GCTGAGG CCTCAGC(-5/-2) BcgI 766 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3782 GTATCC GTATCC(6/5) BclI 4451 TGATCA T^GATCA BfrI 2837 CTTAAG C^TTAAG BlpI 2266 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2266 GCTGAGC GC^T[ACGT]AGC BsaBI 2187 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2929 TCCGGA T^CCGGA BsmBI 4150 CGTCTC CGTCTC(1/5) BsmI 2484 GCATTC GAATGC(1/-1) Bsp1407I 1301 TGTACA T^GTACA BspDI 2709 ATCGAT AT^CGAT BspEI 2929 TCCGGA T^CCGGA BsrGI 1301 TGTACA T^GTACA BssSI 3527 CTCGTG CACGAG(-5/-1) BstEII 4339 GGTGACC G^GT[ACGT]ACC BstPI 4339 GGTGACC G^GT[ACGT]ACC Bsu36I 4414 CCTGAGG CC^T[ACGT]AGG CelII 2266 GCTGAGC GC^T[ACGT]AGC ClaI 2709 ATCGAT AT^CGAT DrdI 981 GACCTGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2757 AGCGCT AGC^GCT Eco81I 4414 CCTGAGG CC^T[ACGT]AGG EcoO65I 4339 GGTGACC G^GT[ACGT]ACC EcoRI 3095 GAATTC G^AATTC EcoRV 1518 GATATC GAT^ATC EcoT22I 3046 ATGCAT ATGCA^T FbaI 4451 TGATCA T^GATCA HpaI 3841 GTTAAC GTT^AAC KasI 2898 GGCGCC G^GCGCC MamI 2187 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2929 TCCGGA T^CCGGA NaeI 2416 GCCGGC GCC^GGC NarI 2898 GGCGCC GG^CGCC NcoI 3084 CCATGG C^CATGG NgoMIV 2416 GCCGGC G^CCGGC NsiI 3046 ATGCAT ATGCA^T PacI 4309 TTAATTAA TTAAT^TAA PinAI 866 ACCGGT A^CCGGT SfoI 2898 GGCGCC GGC^GCC SmaI 65 CCCGGG CCC^GGG SspBI 1301 TGTACA T^GTACA StuI 1531 AGGCCT AGG^CCT XcmI 2754 CCAAGCGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 65 CCCGGG C^CCGGG XmaI 65 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 509 GTAGAC GT^[AC][GT]AC 2092 GTCTAC AcyI 2570 GGCGTC G[GA]^CG[CT]C 2898 GGCGCC AhdI 1147 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3660 GACTGCAAGTC AspEI 1147 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3660 GACTGCAAGTC AspHI 1312 GTGCTC G[AT]GC[AT]^C 3753 GTGCAC BalI 597 TGGCCA TGG^CCA 1254 TGGCCA BamHI 2539 GGATCC G^GATCC 3984 GGATCC BbrPI 8 CACGTG CAC^GTG 1009 CACGTG BglII 365 AGATCT A^GATCT 4193 AGATCT BsaAI 8 CACGTG [CT]AC^GT[GA] 1009 CACGTG BsaHI 2570 GGCGTC G[GA]^CG[CT]C 2898 GGCGCC BsaWI 866 ACCGGT [AT]^CCGG[AT] 2929 TCCGGA BsiHKAI 1312 GTGCTC G[AT]GC[AT]^C 3753 GTGCAC BspHI 1285 TCATGA T^CATGA 4522 TCATGA BsrFI 866 ACCGGT [GA]^CCGG[CT] 2416 GCCGGC BstAPI 3341 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4388 GCAGCCTCTGC BstXI 1738 CCATGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3494 CCATTGGGTTGG Cfr10I 866 ACCGGT [GA]^CCGG[CT] 2416 GCCGGC DraIII 8 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG 26 CACCGCGTG DsaI 348 CCACGG C^C[GA][CT]GG 3084 CCATGG Eam1105I 1147 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3660 GACTGCAAGTC EarI 1726 CTCTTC CTCTTC(1/4) 4085 CTCTTC HaeII 2757 AGCGCT [GA]GCGC^[CT] 2898 GGCGCC Hin1I 2570 GGCGTC G[GA]^CG[CT]C 2898 GGCGCC Ksp632I 1726 CTCTTC CTCTTC(1/4) 4085 CTCTTC MluNI 597 TGGCCA TGG^CCA 1254 TGGCCA MscI 597 TGGCCA TGG^CCA 1254 TGGCCA NdeI 930 CATATG CA^TATG 4003 CATATG PmaCI 8 CACGTG CAC^GTG 1009 CACGTG PmlI 8 CACGTG CAC^GTG 1009 CACGTG RcaI 1285 TCATGA T^CATGA 4522 TCATGA SbfI 299 CCTGCAGG CCTGCA^GG 2653 CCTGCAGG SexAI 567 ACCTGGT A^CC[AT]GGT 4343 ACCAGGT Sse8387I 299 CCTGCAGG CCTGCA^GG 2653 CCTGCAGG XbaI 538 TCTAGA T^CTAGA 4895 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII