################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccII AccIII AflII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AvrII BamHI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiEI BsmBI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstUI BstZ17I CelII ClaI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI HpaI KspI MamI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PmeI PshAI PshBI PvuI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 2097 GGTACC G^GTACC AclI 3278 AACGTT AA^CGTT AocI 884 CCTCAGG CC^T[ACGT]AGG Asp718I 2097 GGTACC G^GTACC AspI 1364 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3591 TGCGCA TGC^GCA BbeI 92 GGCGCC GGCGC^C BbrPI 3545 CACGTG CAC^GTG BbvCI 3323 CCTCAGC CCTCAGC(-5/-2) BcgI 3594 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 3224 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3427 AGATCT A^GATCT BsaAI 3545 CACGTG [CT]AC^GT[GA] BsiWI 1830 CGTACG C^GTACG Bsp1407I 2403 TGTACA T^GTACA BspHI 853 TCATGA T^CATGA BspLU11I 1567 ACATGT A^CATGT BsrDI 3016 CATTGC GCAATG(2/0) BsrGI 2403 TGTACA T^GTACA BstAPI 3151 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1940 GGTGACC G^GT[ACGT]ACC BstPI 1940 GGTGACC G^GT[ACGT]ACC BstXI 2913 CCATCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 884 CCTCAGG CC^T[ACGT]AGG CpoI 3411 CGGACCG CG^G[AT]CCG DrdI 3608 GACACCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 884 CCTCAGG CC^T[ACGT]AGG EcoO65I 1940 GGTGACC G^GT[ACGT]ACC EcoT22I 2744 ATGCAT ATGCA^T FauI 101 GCGGG CCCGC(4/6) FspI 3591 TGCGCA TGC^GCA HincII 2294 GTTGAC GT[CT]^[GA]AC HindII 2294 GTTGAC GT[CT]^[GA]AC KasI 92 GGCGCC G^GCGCC KpnI 2097 GGTACC GGTAC^C NarI 92 GGCGCC GG^CGCC NsiI 2744 ATGCAT ATGCA^T PaeR7I 3194 CTCGAG C^TCGAG PflFI 1364 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3545 CACGTG CAC^GTG PmlI 3545 CACGTG CAC^GTG PpuMI 2767 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 3278 AACGTT AA^CGTT RcaI 853 TCATGA T^CATGA RsrII 3411 CGGACCG CG^G[AT]CCG SacI 3633 GAGCTC GAGCT^C SfoI 92 GGCGCC GGC^GCC SspBI 2403 TGTACA T^GTACA Tth111I 1364 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3194 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 92 GGCGCC G[GA]^CG[CT]C 273 GACGCC AflIII 291 ACACGT A^C[GA][CT]GT 1567 ACATGT AgeI 380 ACCGGT A^CCGGT 1384 ACCGGT AvaI 455 CCCGAG C^[CT]CG[GA]G 3194 CTCGAG BanII 596 GGGCTC G[GA]GC[CT]^C 3633 GAGCTC BciVI 326 GGATAC GTATCC(6/5) 3668 GGATAC BcnI 201 CCCGG CC^[GC]GG 1291 CCCGG BsaHI 92 GGCGCC G[GA]^CG[CT]C 273 GACGCC BseRI 1766 GAGGAG GAGGAG(10/8) 3251 GAGGAG BsgI 1191 CTGCAC GTGCAG(16/14) 2908 CTGCAC BsmI 2316 GAATGC GAATGC(1/-1) 2506 GAATGC BsoBI 455 CCCGAG C^[CT]CG[GA]G 3194 CTCGAG BssSI 12 CACGAG CACGAG(-5/-1) 1270 CACGAG DraI 1547 TTTAAA TTT^AAA 2552 TTTAAA EarI 464 GAAGAG CTCTTC(1/4) 470 GAAGAG HaeII 92 GGCGCC [GA]GCGC^[CT] 708 GGCGCT Hin1I 92 GGCGCC G[GA]^CG[CT]C 273 GACGCC HindIII 1788 AAGCTT A^AGCTT 2559 AAGCTT Ksp632I 464 GAAGAG CTCTTC(1/4) 470 GAAGAG MspA1I 2307 CAGCTG C[AC]G^C[GT]G 2440 CAGCTG NciI 201 CCCGG CC^[GC]GG 1291 CCCGG PinAI 380 ACCGGT A^CCGGT 1384 ACCGGT PvuII 2307 CAGCTG CAG^CTG 2440 CAGCTG SphI 2628 GCATGC GCATG^C 2746 GCATGC XcmI 1893 CCACAATTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3612 CCATGGTCTACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsaWI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII