##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AccIII AflII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AvrII
BamHI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsiEI
BsmBI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstUI BstZ17I CelII
ClaI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI HpaI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PmeI PshAI PshBI
PvuI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2097 GGTACC G^GTACC
AclI 3278 AACGTT AA^CGTT
AocI 884 CCTCAGG CC^T[ACGT]AGG
Asp718I 2097 GGTACC G^GTACC
AspI 1364 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3591 TGCGCA TGC^GCA
BbeI 92 GGCGCC GGCGC^C
BbrPI 3545 CACGTG CAC^GTG
BbvCI 3323 CCTCAGC CCTCAGC(-5/-2)
BcgI 3594 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3224 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3427 AGATCT A^GATCT
BsaAI 3545 CACGTG [CT]AC^GT[GA]
BsiWI 1830 CGTACG C^GTACG
Bsp1407I 2403 TGTACA T^GTACA
BspHI 853 TCATGA T^CATGA
BspLU11I 1567 ACATGT A^CATGT
BsrDI 3016 CATTGC GCAATG(2/0)
BsrGI 2403 TGTACA T^GTACA
BstAPI 3151 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1940 GGTGACC G^GT[ACGT]ACC
BstPI 1940 GGTGACC G^GT[ACGT]ACC
BstXI 2913 CCATCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 884 CCTCAGG CC^T[ACGT]AGG
CpoI 3411 CGGACCG CG^G[AT]CCG
DrdI 3608 GACACCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 884 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1940 GGTGACC G^GT[ACGT]ACC
EcoT22I 2744 ATGCAT ATGCA^T
FauI 101 GCGGG CCCGC(4/6)
FspI 3591 TGCGCA TGC^GCA
HincII 2294 GTTGAC GT[CT]^[GA]AC
HindII 2294 GTTGAC GT[CT]^[GA]AC
KasI 92 GGCGCC G^GCGCC
KpnI 2097 GGTACC GGTAC^C
NarI 92 GGCGCC GG^CGCC
NsiI 2744 ATGCAT ATGCA^T
PaeR7I 3194 CTCGAG C^TCGAG
PflFI 1364 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3545 CACGTG CAC^GTG
PmlI 3545 CACGTG CAC^GTG
PpuMI 2767 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 3278 AACGTT AA^CGTT
RcaI 853 TCATGA T^CATGA
RsrII 3411 CGGACCG CG^G[AT]CCG
SacI 3633 GAGCTC GAGCT^C
SfoI 92 GGCGCC GGC^GCC
SspBI 2403 TGTACA T^GTACA
Tth111I 1364 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3194 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 92 GGCGCC G[GA]^CG[CT]C
273 GACGCC
AflIII 291 ACACGT A^C[GA][CT]GT
1567 ACATGT
AgeI 380 ACCGGT A^CCGGT
1384 ACCGGT
AvaI 455 CCCGAG C^[CT]CG[GA]G
3194 CTCGAG
BanII 596 GGGCTC G[GA]GC[CT]^C
3633 GAGCTC
BciVI 326 GGATAC GTATCC(6/5)
3668 GGATAC
BcnI 201 CCCGG CC^[GC]GG
1291 CCCGG
BsaHI 92 GGCGCC G[GA]^CG[CT]C
273 GACGCC
BseRI 1766 GAGGAG GAGGAG(10/8)
3251 GAGGAG
BsgI 1191 CTGCAC GTGCAG(16/14)
2908 CTGCAC
BsmI 2316 GAATGC GAATGC(1/-1)
2506 GAATGC
BsoBI 455 CCCGAG C^[CT]CG[GA]G
3194 CTCGAG
BssSI 12 CACGAG CACGAG(-5/-1)
1270 CACGAG
DraI 1547 TTTAAA TTT^AAA
2552 TTTAAA
EarI 464 GAAGAG CTCTTC(1/4)
470 GAAGAG
HaeII 92 GGCGCC [GA]GCGC^[CT]
708 GGCGCT
Hin1I 92 GGCGCC G[GA]^CG[CT]C
273 GACGCC
HindIII 1788 AAGCTT A^AGCTT
2559 AAGCTT
Ksp632I 464 GAAGAG CTCTTC(1/4)
470 GAAGAG
MspA1I 2307 CAGCTG C[AC]G^C[GT]G
2440 CAGCTG
NciI 201 CCCGG CC^[GC]GG
1291 CCCGG
PinAI 380 ACCGGT A^CCGGT
1384 ACCGGT
PvuII 2307 CAGCTG CAG^CTG
2440 CAGCTG
SphI 2628 GCATGC GCATG^C
2746 GCATGC
XcmI 1893 CCACAATTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3612 CCATGGTCTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII