##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AviII BalI BbeI
BbrPI BbvCI BcgI BfrI BsaBI
BseAI BsgI BsiWI BsmI BspDI
BspEI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I ClaI CpoI
Eam1105I Eco47III Eco81I EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 38 GACGTC GACGT^C
AccI 1909 GTAGAC GT^[AC][GT]AC
AccII 307 CGCG CG^CG
AcyI 38 GACGTC G[GA]^CG[CT]C
AgeI 549 ACCGGT A^CCGGT
BanI 3412 GGTGCC G^G[CT][GA]CC
BbsI 4331 GAAGAC GAAGAC(2/6)
BciVI 2835 GTATCC GTATCC(6/5)
BclI 252 TGATCA T^GATCA
BglI 1772 GCCCCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 4331 GAAGAC GAAGAC(2/6)
BsaHI 38 GACGTC G[GA]^CG[CT]C
BsiEI 2404 CGGCCG CG[GA][CT]^CG
BsmBI 40 CGTCTC CGTCTC(1/5)
BspLU11I 2860 ACATGT A^CATGT
BsrBI 832 CCGCTC CCGCTC(-3/-3)
BsrDI 2434 CATTGC GCAATG(2/0)
BstEII 2441 GGTCACC G^GT[ACGT]ACC
BstPI 2441 GGTCACC G^GT[ACGT]ACC
BstUI 307 CGCG CG^CG
DrdI 3261 GACTATTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2404 CGGCCG C^GGCCG
EclXI 2404 CGGCCG C^GGCCG
Eco52I 2404 CGGCCG C^GGCCG
EcoNI 1782 CCTTTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2441 GGTCACC G^GT[ACGT]ACC
FbaI 252 TGATCA T^GATCA
HaeII 1978 AGCGCC [GA]GCGC^[CT]
HgaI 3771 GACGC GACGC(5/10)
Hin1I 38 GACGTC G[GA]^CG[CT]C
HincII 3830 GTCAAC GT[CT]^[GA]AC
HindII 3830 GTCAAC GT[CT]^[GA]AC
HindIII 2109 AAGCTT A^AGCTT
MfeI 1153 CAATTG C^AATTG
MunI 1153 CAATTG C^AATTG
MvnI 307 CGCG CG^CG
NaeI 457 GCCGGC GCC^GGC
NcoI 1190 CCATGG C^CATGG
NgoMIV 457 GCCGGC G^CCGGC
PinAI 549 ACCGGT A^CCGGT
PpuMI 4248 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 311 CAGCTG CAG^CTG
SacI 4188 GAGCTC GAGCT^C
SapI 2016 GAAGAGC GCTCTTC(1/4)
ScaI 2745 AGTACT AGT^ACT
SexAI 1581 ACCAGGT A^CC[AT]GGT
SnaBI 2332 TACGTA TAC^GTA
SphI 1120 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 2964 GTGCAC G^TGCAC
3782 GTGCAC
ApaLI 2964 GTGCAC G^TGCAC
3782 GTGCAC
AspI 1580 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1664 GACCGTGTC
AvrII 1787 CCTAGG C^CTAGG
3813 CCTAGG
BlnI 1787 CCTAGG C^CTAGG
3813 CCTAGG
BlpI 2530 GCTCAGC GC^T[ACGT]AGC
4214 GCTTAGC
Bpu1102I 2530 GCTCAGC GC^T[ACGT]AGC
4214 GCTTAGC
BsaAI 2332 TACGTA [CT]AC^GT[GA]
2718 CACGTA
BsaWI 549 ACCGGT [AT]^CCGG[AT]
758 TCCGGT
BseRI 1745 CTCCTC GAGGAG(10/8)
431 GAGGAG
Bsp1407I 2234 TGTACA T^GTACA
2857 TGTACA
BspMI 411 ACCTGC ACCTGC(4/8)
3309 ACCTGC
BsrFI 457 GCCGGC [GA]^CCGG[CT]
549 ACCGGT
BsrGI 2234 TGTACA T^GTACA
2857 TGTACA
BstXI 2547 CCAATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3521 CCAGAGATTTGG
CelII 2530 GCTCAGC GC^T[ACGT]AGC
4214 GCTTAGC
Cfr10I 457 GCCGGC [GA]^CCGG[CT]
549 ACCGGT
DraI 1719 TTTAAA TTT^AAA
4374 TTTAAA
DraIII 170 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4274 CACATGGTG
EaeI 1860 TGGCCG [CT]^GGCC[GA]
2404 CGGCCG
EcoRI 2428 GAATTC G^AATTC
3098 GAATTC
PflFI 1580 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1664 GACCGTGTC
PstI 2652 CTGCAG CTGCA^G
3175 CTGCAG
SmaI 973 CCCGGG CCC^GGG
4158 CCCGGG
SpeI 47 ACTAGT A^CTAGT
2417 ACTAGT
SspBI 2234 TGTACA T^GTACA
2857 TGTACA
StuI 164 AGGCCT AGG^CCT
560 AGGCCT
TaqI 405 TCGA T^CGA
3792 TCGA
Tth111I 1580 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1664 GACCGTGTC
TthHB8I 405 TCGA T^CGA
3792 TCGA
XmaCI 973 CCCGGG C^CCGGG
4158 CCCGGG
XmaI 973 CCCGGG C^CCGGG
4158 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BamHI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII