##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AspI BbeI
BciVI BfrI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiWI Bsp1407I BspEI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII CpoI
DraI Eam1105I Eco47III EcoT22I HaeII
Hin1I HincII HindII HpaI KasI
KspI MamI MfeI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
ApaI 1107 GGGCCC GGGCC^C
AviII 3259 TGCGCA TGC^GCA
BbrPI 1917 CACGTG CAC^GTG
BcgI 310 GCATGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 860 AGATCT A^GATCT
BsaWI 2296 TCCGGT [AT]^CCGG[AT]
BspDI 318 ATCGAT AT^CGAT
BspLU11I 754 ACATGT A^CATGT
BstAPI 2026 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 318 ATCGAT AT^CGAT
DrdI 1733 GACTGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoNI 4741 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4140 GAATTC G^AATTC
EcoRV 4104 GATATC GAT^ATC
FseI 3066 GGCCGGCC GGCCGG^CC
FspI 3259 TGCGCA TGC^GCA
MluI 1957 ACGCGT A^CGCGT
PaeR7I 2995 CTCGAG C^TCGAG
PflMI 4120 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1917 CACGTG CAC^GTG
PmlI 1917 CACGTG CAC^GTG
SbfI 1395 CCTGCAGG CCTGCA^GG
ScaI 156 AGTACT AGT^ACT
SmaI 13 CCCGGG CCC^GGG
Sse8387I 1395 CCTGCAGG CCTGCA^GG
StuI 3919 AGGCCT AGG^CCT
Van91I 4120 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2995 CTCGAG C^TCGAG
XmaCI 13 CCCGGG C^CCGGG
XmaI 13 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 3211 GGTACC G^GTACC
3997 GGTACC
AccI 3300 GTCTAC GT^[AC][GT]AC
3665 GTCTAC
Asp718I 3211 GGTACC G^GTACC
3997 GGTACC
AvaI 13 CCCGGG C^[CT]CG[GA]G
2995 CTCGAG
BamHI 626 GGATCC G^GATCC
1348 GGATCC
BbvCI 1744 CCTCAGC CCTCAGC(-5/-2)
3606 CCTCAGC
BsaI 3712 GAGACC GGTCTC(1/5)
1249 GGTCTC
BsiEI 5 CGGCCG CG[GA][CT]^CG
3194 CGGTCG
BsmI 2578 GCATTC GAATGC(1/-1)
2664 GCATTC
BsoBI 13 CCCGGG C^[CT]CG[GA]G
2995 CTCGAG
BsrDI 431 CATTGC GCAATG(2/0)
3502 GCAATG
BstEII 1340 GGTTACC G^GT[ACGT]ACC
4131 GGTCACC
BstPI 1340 GGTTACC G^GT[ACGT]ACC
4131 GGTCACC
EcoO65I 1340 GGTTACC G^GT[ACGT]ACC
4131 GGTCACC
HindIII 889 AAGCTT A^AGCTT
939 AAGCTT
KpnI 3211 GGTACC GGTAC^C
3997 GGTACC
PvuII 2124 CAGCTG CAG^CTG
4006 CAGCTG
SacI 2998 GAGCTC GAGCT^C
4035 GAGCTC
SapI 1464 GAAGAGC GCTCTTC(1/4)
1973 GAAGAGC
SexAI 1801 ACCAGGT A^CC[AT]GGT
3613 ACCAGGT
SfiI 1037 GGCCTTACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3057 GGCCGTTGGGGCC
SmlI 2995 CTCGAG C^T[CT][GA]AG
3585 CTTGAG
SphI 2078 GCATGC GCATG^C
3774 GCATGC
XbaI 65 TCTAGA T^CTAGA
1175 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaII AvrII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII