##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII AvrII BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspHI
BsrBI BsrGI BssHII BstBI BstEII
BstPI CelII DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HpaI KspI MamI
MfeI MluI MroI MunI NheI
NotI NruI NspV PacI PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AgeI 4112 ACCGGT A^CCGGT
AocI 1337 CCTGAGG CC^T[ACGT]AGG
AspI 2771 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2026 GGATCC G^GATCC
BbeI 3266 GGCGCC GGCGC^C
BclI 1906 TGATCA T^GATCA
BsaWI 4112 ACCGGT [AT]^CCGG[AT]
BspDI 371 ATCGAT AT^CGAT
BssSI 3008 CACGAG CACGAG(-5/-1)
Bst1107I 3618 GTATAC GTA^TAC
BstZ17I 3618 GTATAC GTA^TAC
Bsu36I 1337 CCTGAGG CC^T[ACGT]AGG
ClaI 371 ATCGAT AT^CGAT
CpoI 4114 CGGTCCG CG^G[AT]CCG
DrdI 710 GACAGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1337 CCTGAGG CC^T[ACGT]AGG
EcoRI 685 GAATTC G^AATTC
EcoT22I 1471 ATGCAT ATGCA^T
FbaI 1906 TGATCA T^GATCA
KasI 3266 GGCGCC G^GCGCC
NaeI 4586 GCCGGC GCC^GGC
NarI 3266 GGCGCC GG^CGCC
NdeI 3050 CATATG CA^TATG
NgoMIV 4586 GCCGGC G^CCGGC
NsiI 1471 ATGCAT ATGCA^T
PflFI 2771 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4112 ACCGGT A^CCGGT
RsrII 4114 CGGTCCG CG^G[AT]CCG
SfoI 3266 GGCGCC GGC^GCC
SphI 3967 GCATGC GCATG^C
Tth111I 2771 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
BspLU11I 71 ACATGT A^CATGT
2535 ACATGT
BsrFI 4112 ACCGGT [GA]^CCGG[CT]
4586 GCCGGC
Cfr10I 4112 ACCGGT [GA]^CCGG[CT]
4586 GCCGGC
HaeII 3266 GGCGCC [GA]GCGC^[CT]
4128 GGCGCT
HincII 870 GTCAAC GT[CT]^[GA]AC
4769 GTCAAC
HindII 870 GTCAAC GT[CT]^[GA]AC
4769 GTCAAC
PpuMI 1257 AGGTCCT [GA]G^G[AT]CC[CT]
5352 GGGTCCT
StuI 774 AGGCCT AGG^CCT
3069 AGGCCT
XbaI 4174 TCTAGA T^CTAGA
4813 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PmaCI
PmlI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI