################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccIII AclI AflII AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII AvrII BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII BstPI CelII DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HpaI KspI MamI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AgeI 4112 ACCGGT A^CCGGT AocI 1337 CCTGAGG CC^T[ACGT]AGG AspI 2771 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2026 GGATCC G^GATCC BbeI 3266 GGCGCC GGCGC^C BclI 1906 TGATCA T^GATCA BsaWI 4112 ACCGGT [AT]^CCGG[AT] BspDI 371 ATCGAT AT^CGAT BssSI 3008 CACGAG CACGAG(-5/-1) Bst1107I 3618 GTATAC GTA^TAC BstZ17I 3618 GTATAC GTA^TAC Bsu36I 1337 CCTGAGG CC^T[ACGT]AGG ClaI 371 ATCGAT AT^CGAT CpoI 4114 CGGTCCG CG^G[AT]CCG DrdI 710 GACAGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1337 CCTGAGG CC^T[ACGT]AGG EcoRI 685 GAATTC G^AATTC EcoT22I 1471 ATGCAT ATGCA^T FbaI 1906 TGATCA T^GATCA KasI 3266 GGCGCC G^GCGCC NaeI 4586 GCCGGC GCC^GGC NarI 3266 GGCGCC GG^CGCC NdeI 3050 CATATG CA^TATG NgoMIV 4586 GCCGGC G^CCGGC NsiI 1471 ATGCAT ATGCA^T PflFI 2771 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4112 ACCGGT A^CCGGT RsrII 4114 CGGTCCG CG^G[AT]CCG SfoI 3266 GGCGCC GGC^GCC SphI 3967 GCATGC GCATG^C Tth111I 2771 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BspLU11I 71 ACATGT A^CATGT 2535 ACATGT BsrFI 4112 ACCGGT [GA]^CCGG[CT] 4586 GCCGGC Cfr10I 4112 ACCGGT [GA]^CCGG[CT] 4586 GCCGGC HaeII 3266 GGCGCC [GA]GCGC^[CT] 4128 GGCGCT HincII 870 GTCAAC GT[CT]^[GA]AC 4769 GTCAAC HindII 870 GTCAAC GT[CT]^[GA]AC 4769 GTCAAC PpuMI 1257 AGGTCCT [GA]G^G[AT]CC[CT] 5352 GGGTCCT StuI 774 AGGCCT AGG^CCT 3069 AGGCCT XbaI 4174 TCTAGA T^CTAGA 4813 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 144 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PmaCI PmlI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI