##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII BbeI BbrPI
BbvCI BcgI BglII BsaAI BsaHI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrDI BsrFI BssHII BssSI
BstBI BstUI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I FseI FspI Hin1I KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Van91I
XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 3499 AACGTT AA^CGTT
AflII 2790 CTTAAG C^TTAAG
Alw44I 409 GTGCAC G^TGCAC
Aor51HI 1554 AGCGCT AGC^GCT
ApaI 3960 GGGCCC GGGCC^C
ApaLI 409 GTGCAC G^TGCAC
AvrII 2104 CCTAGG C^CTAGG
BanI 504 GGCACC G^G[CT][GA]CC
BciVI 231 GTATCC GTATCC(6/5)
BfrI 2790 CTTAAG C^TTAAG
BglI 2048 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2104 CCTAGG C^CTAGG
BsaWI 957 ACCGGA [AT]^CCGG[AT]
BsmI 3108 GCATTC GAATGC(1/-1)
Bsp1407I 3667 TGTACA T^GTACA
BspMI 4102 GCAGGT ACCTGC(4/8)
BsrGI 3667 TGTACA T^GTACA
Bst1107I 4141 GTATAC GTA^TAC
BstEII 3483 GGTTACC G^GT[ACGT]ACC
BstPI 3483 GGTTACC G^GT[ACGT]ACC
BstZ17I 4141 GTATAC GTA^TAC
Eco47III 1554 AGCGCT AGC^GCT
EcoO65I 3483 GGTTACC G^GT[ACGT]ACC
EcoRI 2416 GAATTC G^AATTC
EcoRV 911 GATATC GAT^ATC
EcoT22I 4186 ATGCAT ATGCA^T
HpaI 4751 GTTAAC GTT^AAC
MfeI 3141 CAATTG C^AATTG
MunI 3141 CAATTG C^AATTG
NcoI 2870 CCATGG C^CATGG
NdeI 1215 CATATG CA^TATG
NheI 3353 GCTAGC G^CTAGC
NsiI 4186 ATGCAT ATGCA^T
PaeR7I 734 CTCGAG C^TCGAG
Psp1406I 3499 AACGTT AA^CGTT
SmaI 1516 CCCGGG CCC^GGG
SspBI 3667 TGTACA T^GTACA
SspI 4483 AATATT AAT^ATT
StuI 3821 AGGCCT AGG^CCT
XhoI 734 CTCGAG C^TCGAG
XmaCI 1516 CCCGGG C^CCGGG
XmaI 1516 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 4141 GTATAC GT^[AC][GT]AC
4806 GTAGAC
AocI 143 CCTGAGG CC^T[ACGT]AGG
4324 CCTCAGG
Asp700I 2451 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2679 GAAATCCTTC
AspI 1066 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3714 GACCAAGTC
BamHI 30 GGATCC G^GATCC
4583 GGATCC
BsaBI 1595 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4108 GATTAAAATC
BsaI 258 GAGACC GGTCTC(1/5)
1616 GGTCTC
BsrBI 1566 CCGCTC CCGCTC(-3/-3)
1 GAGCGG
BstAPI 2553 GCATTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3465 GCAAAAATTGC
Bsu36I 143 CCTGAGG CC^T[ACGT]AGG
4324 CCTCAGG
Eco81I 143 CCTGAGG CC^T[ACGT]AGG
4324 CCTCAGG
EcoNI 970 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2684 CCTTCTGAAGG
HaeII 39 AGCGCC [GA]GCGC^[CT]
1554 AGCGCT
HgaI 613 GACGC GACGC(5/10)
1489 GCGTC
HindIII 273 AAGCTT A^AGCTT
998 AAGCTT
MaeII 2837 ACGT A^CGT
3500 ACGT
MamI 1595 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4108 GATTAAAATC
PflFI 1066 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3714 GACCAAGTC
PshAI 1178 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1375 GACTCCAGTC
SacI 2151 GAGCTC GAGCT^C
2714 GAGCTC
SphI 471 GCATGC GCATG^C
547 GCATGC
Tth111I 1066 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3714 GACCAAGTC
XcmI 1485 CCAAGCGTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2043 CCAGTGCCGTGGTGG
XmnI 2451 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2679 GAAATCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII