##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
ApaI ApaLI AscI Asp718I AspEI
BbrPI BciVI BfrI BsiWI BsmBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI HincII HindII
HpaI KpnI KspI MfeI MluI
MunI NaeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2873 GACGTC GACGT^C
AccI 1057 GTAGAC GT^[AC][GT]AC
AccII 4225 CGCG CG^CG
AccIII 2676 TCCGGA T^CCGGA
AclI 2113 AACGTT AA^CGTT
Aor51HI 3047 AGCGCT AGC^GCT
AspI 4294 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3166 CCTAGG C^CTAGG
BamHI 2679 GGATCC G^GATCC
BcgI 2935 GCACCTCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3166 CCTAGG C^CTAGG
BsaAI 5257 TACGTG [CT]AC^GT[GA]
BsaBI 2119 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2676 TCCGGA T^CCGGA
BsiEI 4878 CGGTCG CG[GA][CT]^CG
Bsp1407I 1037 TGTACA T^GTACA
BspEI 2676 TCCGGA T^CCGGA
BsrBI 4006 GAGCGG CCGCTC(-3/-3)
BsrGI 1037 TGTACA T^GTACA
BstAPI 3655 GCATCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4225 CGCG CG^CG
BstXI 398 CCAAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 3047 AGCGCT AGC^GCT
HgaI 5282 GCGTC GACGC(5/10)
MamI 2119 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2676 TCCGGA T^CCGGA
MvnI 4225 CGCG CG^CG
NheI 1227 GCTAGC G^CTAGC
PflFI 4294 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2113 AACGTT AA^CGTT
ScaI 4716 AGTACT AGT^ACT
SexAI 4076 ACCAGGT A^CC[AT]GGT
SmaI 4303 CCCGGG CCC^GGG
SphI 393 GCATGC GCATG^C
SspBI 1037 TGTACA T^GTACA
SspI 5122 AATATT AAT^ATT
Tth111I 4294 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 104 CCAGCGAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4303 CCCGGG C^CCGGG
XmaI 4303 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 2887 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3196 GAAGATATTC
AvaI 2958 CCCGAG C^[CT]CG[GA]G
4303 CCCGGG
BbeI 118 GGCGCC GGCGC^C
680 GGCGCC
BclI 145 TGATCA T^GATCA
5472 TGATCA
BglI 3364 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3991 GCCCAGCTGGC
BsoBI 2958 CCCGAG C^[CT]CG[GA]G
4303 CCCGGG
BspDI 1604 ATCGAT AT^CGAT
4474 ATCGAT
BspLU11I 4547 ACATGT A^CATGT
5206 ACATGT
BssSI 1963 CACGAG CACGAG(-5/-1)
3314 CTCGTG
ClaI 1604 ATCGAT AT^CGAT
4474 ATCGAT
FbaI 145 TGATCA T^GATCA
5472 TGATCA
KasI 118 GGCGCC G^GCGCC
680 GGCGCC
MslI 4260 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5201 CATGGACATG
NarI 118 GGCGCC GG^CGCC
680 GGCGCC
NdeI 1660 CATATG CA^TATG
3954 CATATG
SfoI 118 GGCGCC GGC^GCC
680 GGCGCC
StuI 2403 AGGCCT AGG^CCT
3031 AGGCCT
XbaI 2974 TCTAGA T^CTAGA
4791 TCTAGA
XmnI 2887 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3196 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII