################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AocI AscI AseI AsnI Asp718I AspI AvrII BalI BamHI BcgI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BstBI BstEII BstPI Bsu36I ClaI CpoI Eco81I EcoO65I EcoRV FseI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SpeI Sse8387I SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 4236 GACGTC GACGT^C AhdI 3297 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3492 AGCGCT AGC^GCT ApaI 137 GGGCCC GGGCC^C AspEI 3297 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4090 TGCGCA TGC^GCA BbrPI 2012 CACGTG CAC^GTG BbvCI 2835 CCTCAGC CCTCAGC(-5/-2) BclI 368 TGATCA T^GATCA BglI 72 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 574 GAGACC GGTCTC(1/5) BsiEI 107 CGGCCG CG[GA][CT]^CG BspHI 965 TCATGA T^CATGA BssSI 3764 CTCGTG CACGAG(-5/-1) Bst1107I 567 GTATAC GTA^TAC BstXI 1502 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1622 GGATCT [GA]^GATC[CT] BstZ17I 567 GTATAC GTA^TAC DrdI 3443 GACTTGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 133 CCGTGG C^C[GA][CT]GG EaeI 107 CGGCCG [CT]^GGCC[GA] EagI 107 CGGCCG C^GGCCG Eam1105I 3297 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 107 CGGCCG C^GGCCG Eco47III 3492 AGCGCT AGC^GCT Eco52I 107 CGGCCG C^GGCCG EcoT22I 4821 ATGCAT ATGCA^T FbaI 368 TGATCA T^GATCA FspI 4090 TGCGCA TGC^GCA HpaI 2319 GTTAAC GTT^AAC MflI 1622 GGATCT [GA]^GATC[CT] NsiI 4821 ATGCAT ATGCA^T PaeR7I 4699 CTCGAG C^TCGAG PmaCI 2012 CACGTG CAC^GTG PmlI 2012 CACGTG CAC^GTG PpuMI 2575 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1544 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 965 TCATGA T^CATGA SnaBI 543 TACGTA TAC^GTA SspI 1881 AATATT AAT^ATT XcmI 3154 CCAAAATGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 4699 CTCGAG C^TCGAG XhoII 1622 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AvaII 402 GGTCC G^G[AT]CC 2576 GGACC BbeI 46 GGCGCC GGCGC^C 80 GGCGCC BsmBI 3970 CGTCTC CGTCTC(1/5) 3927 GAGACG BsmI 225 GAATGC GAATGC(1/-1) 3677 GCATTC Bsp1407I 501 TGTACA T^GTACA 876 TGTACA BspMI 3414 ACCTGC ACCTGC(4/8) 3899 ACCTGC BsrFI 105 GCCGGC [GA]^CCGG[CT] 2312 GCCGGC BsrGI 501 TGTACA T^GTACA 876 TGTACA BssHII 68 GCGCGC G^CGCGC 112 GCGCGC Cfr10I 105 GCCGGC [GA]^CCGG[CT] 2312 GCCGGC DraII 137 GGGCCCT [GA]G^G[ACGT]CC[CT] 2575 AGGACCC EcoNI 2606 CCTTACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4391 CCTTCTTCAGG EcoO109I 137 GGGCCCT [GA]G^G[ACGT]CC[CT] 2575 AGGACCC EcoRI 2202 GAATTC G^AATTC 3977 GAATTC FauI 3855 CCCGC CCCGC(4/6) 59 GCGGG HincII 2319 GTTAAC GT[CT]^[GA]AC 2385 GTTGAC HindII 2319 GTTAAC GT[CT]^[GA]AC 2385 GTTGAC HindIII 1681 AAGCTT A^AGCTT 2664 AAGCTT KasI 46 GGCGCC G^GCGCC 80 GGCGCC NaeI 105 GCCGGC GCC^GGC 2312 GCCGGC NarI 46 GGCGCC GG^CGCC 80 GGCGCC NgoMIV 105 GCCGGC G^CCGGC 2312 GCCGGC SacI 2934 GAGCTC GAGCT^C 3346 GAGCTC ScaI 628 AGTACT AGT^ACT 4102 AGTACT SfoI 46 GGCGCC GGC^GCC 80 GGCGCC SphI 1904 GCATGC GCATG^C 2236 GCATGC SspBI 501 TGTACA T^GTACA 876 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraIII EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmnI