##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AocI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BcgI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BstBI BstEII BstPI Bsu36I ClaI
CpoI Eco81I EcoO65I EcoRV FseI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SpeI Sse8387I SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 4236 GACGTC GACGT^C
AhdI 3297 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3492 AGCGCT AGC^GCT
ApaI 137 GGGCCC GGGCC^C
AspEI 3297 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4090 TGCGCA TGC^GCA
BbrPI 2012 CACGTG CAC^GTG
BbvCI 2835 CCTCAGC CCTCAGC(-5/-2)
BclI 368 TGATCA T^GATCA
BglI 72 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 574 GAGACC GGTCTC(1/5)
BsiEI 107 CGGCCG CG[GA][CT]^CG
BspHI 965 TCATGA T^CATGA
BssSI 3764 CTCGTG CACGAG(-5/-1)
Bst1107I 567 GTATAC GTA^TAC
BstXI 1502 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1622 GGATCT [GA]^GATC[CT]
BstZ17I 567 GTATAC GTA^TAC
DrdI 3443 GACTTGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 133 CCGTGG C^C[GA][CT]GG
EaeI 107 CGGCCG [CT]^GGCC[GA]
EagI 107 CGGCCG C^GGCCG
Eam1105I 3297 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 107 CGGCCG C^GGCCG
Eco47III 3492 AGCGCT AGC^GCT
Eco52I 107 CGGCCG C^GGCCG
EcoT22I 4821 ATGCAT ATGCA^T
FbaI 368 TGATCA T^GATCA
FspI 4090 TGCGCA TGC^GCA
HpaI 2319 GTTAAC GTT^AAC
MflI 1622 GGATCT [GA]^GATC[CT]
NsiI 4821 ATGCAT ATGCA^T
PaeR7I 4699 CTCGAG C^TCGAG
PmaCI 2012 CACGTG CAC^GTG
PmlI 2012 CACGTG CAC^GTG
PpuMI 2575 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1544 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 965 TCATGA T^CATGA
SnaBI 543 TACGTA TAC^GTA
SspI 1881 AATATT AAT^ATT
XcmI 3154 CCAAAATGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4699 CTCGAG C^TCGAG
XhoII 1622 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 402 GGTCC G^G[AT]CC
2576 GGACC
BbeI 46 GGCGCC GGCGC^C
80 GGCGCC
BsmBI 3970 CGTCTC CGTCTC(1/5)
3927 GAGACG
BsmI 225 GAATGC GAATGC(1/-1)
3677 GCATTC
Bsp1407I 501 TGTACA T^GTACA
876 TGTACA
BspMI 3414 ACCTGC ACCTGC(4/8)
3899 ACCTGC
BsrFI 105 GCCGGC [GA]^CCGG[CT]
2312 GCCGGC
BsrGI 501 TGTACA T^GTACA
876 TGTACA
BssHII 68 GCGCGC G^CGCGC
112 GCGCGC
Cfr10I 105 GCCGGC [GA]^CCGG[CT]
2312 GCCGGC
DraII 137 GGGCCCT [GA]G^G[ACGT]CC[CT]
2575 AGGACCC
EcoNI 2606 CCTTACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4391 CCTTCTTCAGG
EcoO109I 137 GGGCCCT [GA]G^G[ACGT]CC[CT]
2575 AGGACCC
EcoRI 2202 GAATTC G^AATTC
3977 GAATTC
FauI 3855 CCCGC CCCGC(4/6)
59 GCGGG
HincII 2319 GTTAAC GT[CT]^[GA]AC
2385 GTTGAC
HindII 2319 GTTAAC GT[CT]^[GA]AC
2385 GTTGAC
HindIII 1681 AAGCTT A^AGCTT
2664 AAGCTT
KasI 46 GGCGCC G^GCGCC
80 GGCGCC
NaeI 105 GCCGGC GCC^GGC
2312 GCCGGC
NarI 46 GGCGCC GG^CGCC
80 GGCGCC
NgoMIV 105 GCCGGC G^CCGGC
2312 GCCGGC
SacI 2934 GAGCTC GAGCT^C
3346 GAGCTC
ScaI 628 AGTACT AGT^ACT
4102 AGTACT
SfoI 46 GGCGCC GGC^GCC
80 GGCGCC
SphI 1904 GCATGC GCATG^C
2236 GCATGC
SspBI 501 TGTACA T^GTACA
876 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaLI ApoI Asp700I AspHI
AvaI BanI BanII BbsI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraIII EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmnI