##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AclI AgeI AhdI Aor51HI
AscI Asp700I AspEI AvaI BcgI
BsaBI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FseI HpaI KspI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 4234 GACGTC GACGT^C
Acc65I 336 GGTACC G^GTACC
AccII 362 CGCG CG^CG
AcsI 3009 GAATTT [GA]^AATT[CT]
ApaI 4248 GGGCCC GGGCC^C
ApoI 3009 GAATTT [GA]^AATT[CT]
AseI 1756 ATTAAT AT^TAAT
AsnI 1756 ATTAAT AT^TAAT
Asp718I 336 GGTACC G^GTACC
AspI 2262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 244 TGCGCA TGC^GCA
AvrII 3240 CCTAGG C^CTAGG
BamHI 1874 GGATCC G^GATCC
BbeI 3621 GGCGCC GGCGC^C
BglII 1298 AGATCT A^GATCT
BlnI 3240 CCTAGG C^CTAGG
BsmBI 3779 CGTCTC CGTCTC(1/5)
BssSI 987 CTCGTG CACGAG(-5/-1)
BstEII 1652 GGTGACC G^GT[ACGT]ACC
BstPI 1652 GGTGACC G^GT[ACGT]ACC
BstUI 362 CGCG CG^CG
DrdI 396 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1652 GGTGACC G^GT[ACGT]ACC
EcoT22I 3497 ATGCAT ATGCA^T
FauI 363 GCGGG CCCGC(4/6)
FspI 244 TGCGCA TGC^GCA
HaeII 3621 GGCGCC [GA]GCGC^[CT]
KasI 3621 GGCGCC G^GCGCC
KpnI 336 GGTACC GGTAC^C
MvnI 362 CGCG CG^CG
NarI 3621 GGCGCC GG^CGCC
NsiI 3497 ATGCAT ATGCA^T
PflFI 2262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1756 ATTAAT AT^TAAT
ScaI 1483 AGTACT AGT^ACT
SfoI 3621 GGCGCC GGC^GCC
TaqI 664 TCGA T^CGA
Tth111I 2262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 664 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 61 TCCGGA T^CCGGA
968 TCCGGA
AocI 812 CCTTAGG CC^T[ACGT]AGG
3474 CCTCAGG
BbrPI 93 CACGTG CAC^GTG
210 CACGTG
BbvCI 744 GCTGAGG CCTCAGC(-5/-2)
1184 GCTGAGG
BciVI 2317 GGATAC GTATCC(6/5)
2356 GGATAC
BcnI 773 CCGGG CC^[GC]GG
2845 CCGGG
BglI 1492 GCCACTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4124 GCCATGTGGGC
BlpI 1684 GCTTAGC GC^T[ACGT]AGC
2453 GCTTAGC
Bpu1102I 1684 GCTTAGC GC^T[ACGT]AGC
2453 GCTTAGC
BsaAI 93 CACGTG [CT]AC^GT[GA]
210 CACGTG
BsaI 44 GGTCTC GGTCTC(1/5)
693 GGTCTC
BseAI 61 TCCGGA T^CCGGA
968 TCCGGA
BsgI 1469 CTGCAC GTGCAG(16/14)
3460 CTGCAC
BsmI 1055 GCATTC GAATGC(1/-1)
3835 GCATTC
BspEI 61 TCCGGA T^CCGGA
968 TCCGGA
BsrBI 332 GAGCGG CCGCTC(-3/-3)
3445 GAGCGG
Bsu36I 812 CCTTAGG CC^T[ACGT]AGG
3474 CCTCAGG
CelII 1684 GCTTAGC GC^T[ACGT]AGC
2453 GCTTAGC
CfoI 245 GCGC GCG^C
3622 GCGC
DraIII 2895 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3435 CACATTGTG
Eco81I 812 CCTTAGG CC^T[ACGT]AGG
3474 CCTCAGG
HhaI 245 GCGC GCG^C
3622 GCGC
HinP1I 245 GCGC G^CGC
3622 GCGC
HincII 11 GTTGAC GT[CT]^[GA]AC
528 GTCAAC
HindII 11 GTTGAC GT[CT]^[GA]AC
528 GTCAAC
HindIII 1682 AAGCTT A^AGCTT
4106 AAGCTT
MroI 61 TCCGGA T^CCGGA
968 TCCGGA
NciI 773 CCGGG CC^[GC]GG
2845 CCGGG
NcoI 701 CCATGG C^CATGG
4243 CCATGG
PflMI 3693 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4051 CCATCCTCTGG
PmaCI 93 CACGTG CAC^GTG
210 CACGTG
PmlI 93 CACGTG CAC^GTG
210 CACGTG
SapI 1172 GAAGAGC GCTCTTC(1/4)
2728 GAAGAGC
SphI 3495 GCATGC GCATG^C
3879 GCATGC
Van91I 3693 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4051 CCATCCTCTGG
XbaI 549 TCTAGA T^CTAGA
1505 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcyI AfaI AflII AflIII
AluI Alw44I AlwI AlwNI ApaLI
AspHI AvaII BalI BanI BanII
BbsI BbvI BclI BfaI BfrI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII