##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AcsI AflIII
AgeI AhdI ApoI AscI AseI
AsnI AspEI AviII BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I ClaI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FseI FspI HpaI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI SacI SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tsp509I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 3141 GGTACC G^GTACC
AccI 5000 GTAGAC GT^[AC][GT]AC
AflII 2191 CTTAAG C^TTAAG
Alw44I 4019 GTGCAC G^TGCAC
ApaLI 4019 GTGCAC G^TGCAC
Asp718I 3141 GGTACC G^GTACC
AspI 3725 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 852 CCTAGG C^CTAGG
BbrPI 1831 CACGTG CAC^GTG
BcgI 375 GCAAGAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2107 GTATCC GTATCC(6/5)
BclI 1235 TGATCA T^GATCA
BfrI 2191 CTTAAG C^TTAAG
BglII 1959 AGATCT A^GATCT
BlnI 852 CCTAGG C^CTAGG
BsaAI 1831 CACGTG [CT]AC^GT[GA]
BsaWI 1136 ACCGGA [AT]^CCGG[AT]
BsmI 3247 GCATTC GAATGC(1/-1)
BspHI 2118 TCATGA T^CATGA
CpoI 2726 CGGTCCG CG^G[AT]CCG
DrdI 1012 GACCCCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5166 ATGCAT ATGCA^T
FbaI 1235 TGATCA T^GATCA
HindIII 1885 AAGCTT A^AGCTT
KpnI 3141 GGTACC GGTAC^C
KspI 2879 CCGCGG CCGC^GG
NaeI 58 GCCGGC GCC^GGC
NgoMIV 58 GCCGGC G^CCGGC
NsiI 5166 ATGCAT ATGCA^T
PflFI 3725 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1831 CACGTG CAC^GTG
PmlI 1831 CACGTG CAC^GTG
RcaI 2118 TCATGA T^CATGA
RsrII 2726 CGGTCCG CG^G[AT]CCG
SacII 2879 CCGCGG CCGC^GG
SexAI 3726 ACCTGGT A^CC[AT]GGT
SfiI 4554 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 3725 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2319 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 61 GGCGCC G[GA]^CG[CT]C
3848 GGCGCC
Aor51HI 305 AGCGCT AGC^GCT
4047 AGCGCT
BamHI 3646 GGATCC G^GATCC
3661 GGATCC
BbeI 61 GGCGCC GGCGC^C
3848 GGCGCC
BsaHI 61 GGCGCC G[GA]^CG[CT]C
3848 GGCGCC
BsiEI 40 CGACCG CG[GA][CT]^CG
2871 CGACCG
BsrFI 58 GCCGGC [GA]^CCGG[CT]
1529 ACCGGC
BstEII 871 GGTGACC G^GT[ACGT]ACC
3764 GGTCACC
BstPI 871 GGTGACC G^GT[ACGT]ACC
3764 GGTCACC
BstXI 428 CCAGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4064 CCAGACTCCTGG
Cfr10I 58 GCCGGC [GA]^CCGG[CT]
1529 ACCGGC
Eco47III 305 AGCGCT AGC^GCT
4047 AGCGCT
EcoO65I 871 GGTGACC G^GT[ACGT]ACC
3764 GGTCACC
Hin1I 61 GGCGCC G[GA]^CG[CT]C
3848 GGCGCC
HincII 3461 GTTGAC GT[CT]^[GA]AC
3620 GTCAAC
HindII 3461 GTTGAC GT[CT]^[GA]AC
3620 GTCAAC
KasI 61 GGCGCC G^GCGCC
3848 GGCGCC
MslI 3539 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4777 CATCTCAGTG
NarI 61 GGCGCC GG^CGCC
3848 GGCGCC
PshAI 1602 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3201 GACCCTGGTC
SfoI 61 GGCGCC GGC^GCC
3848 GGCGCC
SphI 1707 GCATGC GCATG^C
4041 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI