##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AclI Aor51HI AscI
AseI AsnI AspI AvrII BamHI
BbvCI BlnI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BssSI Bst1107I
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
HincII HindII HpaI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PflFI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SfiI SgrAI SmaI SnaBI
SpeI StuI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflII 3714 CTTAAG C^TTAAG
AgeI 2730 ACCGGT A^CCGGT
AhdI 926 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3288 GTGCAC G^TGCAC
ApaI 1938 GGGCCC GGGCC^C
ApaLI 3288 GTGCAC G^TGCAC
AspEI 926 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1516 TGCGCA TGC^GCA
BbeI 1032 GGCGCC GGCGC^C
BbrPI 2835 CACGTG CAC^GTG
BcgI 1253 CGAAATATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 566 GGATAC GTATCC(6/5)
BclI 1453 TGATCA T^GATCA
BfrI 3714 CTTAAG C^TTAAG
BglII 2463 AGATCT A^GATCT
BlpI 2329 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2329 GCTGAGC GC^T[ACGT]AGC
BsaWI 2730 ACCGGT [AT]^CCGG[AT]
Bsp1407I 2778 TGTACA T^GTACA
BspHI 1698 TCATGA T^CATGA
BspLU11I 2781 ACATGT A^CATGT
BsrBI 1186 GAGCGG CCGCTC(-3/-3)
BsrFI 2730 ACCGGT [GA]^CCGG[CT]
BsrGI 2778 TGTACA T^GTACA
BssHII 599 GCGCGC G^CGCGC
BstBI 1251 TTCGAA TT^CGAA
BstXI 2804 CCATAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2463 AGATCT [GA]^GATC[CT]
CelII 2329 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2730 ACCGGT [GA]^CCGG[CT]
DrdI 2543 GACTTTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 926 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3358 CCTTGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1453 TGATCA T^GATCA
FspI 1516 TGCGCA TGC^GCA
HaeII 1032 GGCGCC [GA]GCGC^[CT]
HindIII 2015 AAGCTT A^AGCTT
KasI 1032 GGCGCC G^GCGCC
MflI 2463 AGATCT [GA]^GATC[CT]
NarI 1032 GGCGCC GG^CGCC
NspV 1251 TTCGAA TT^CGAA
PinAI 2730 ACCGGT A^CCGGT
PmaCI 2835 CACGTG CAC^GTG
PmlI 2835 CACGTG CAC^GTG
RcaI 1698 TCATGA T^CATGA
SbfI 1651 CCTGCAGG CCTGCA^GG
SfoI 1032 GGCGCC GGC^GCC
SfuI 1251 TTCGAA TT^CGAA
SphI 1407 GCATGC GCATG^C
Sse8387I 1651 CCTGCAGG CCTGCA^GG
SspBI 2778 TGTACA T^GTACA
XhoII 2463 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1194 GGTACC G^GTACC
2436 GGTACC
AccI 1586 GTAGAC GT^[AC][GT]AC
3555 GTAGAC
AccII 600 CGCG CG^CG
683 CGCG
AcsI 3520 AAATTT [GA]^AATT[CT]
3829 GAATTT
AcyI 1032 GGCGCC G[GA]^CG[CT]C
2800 GACGCC
AflIII 42 ACACGT A^C[GA][CT]GT
2781 ACATGT
AlwI 2383 GATCC GGATC(4/5)
1978 GGATC
AocI 1475 CCTCAGG CC^T[ACGT]AGG
2885 CCTGAGG
ApoI 3520 AAATTT [GA]^AATT[CT]
3829 GAATTT
Asp700I 1742 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2489 GAACACATTC
Asp718I 1194 GGTACC G^GTACC
2436 GGTACC
BbsI 1915 GAAGAC GAAGAC(2/6)
3161 GTCTTC
BglI 2939 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3764 GCCTGTGTGGC
BpuAI 1915 GAAGAC GAAGAC(2/6)
3161 GTCTTC
BsaAI 43 CACGTA [CT]AC^GT[GA]
2835 CACGTG
BsaHI 1032 GGCGCC G[GA]^CG[CT]C
2800 GACGCC
BsmBI 1727 CGTCTC CGTCTC(1/5)
11 GAGACG
BsmI 3010 GAATGC GAATGC(1/-1)
3461 GCATTC
BsrDI 561 GCAATG GCAATG(2/0)
3915 GCAATG
BstEII 491 GGTGACC G^GT[ACGT]ACC
1052 GGTGACC
BstPI 491 GGTGACC G^GT[ACGT]ACC
1052 GGTGACC
BstUI 600 CGCG CG^CG
683 CGCG
Bsu36I 1475 CCTCAGG CC^T[ACGT]AGG
2885 CCTGAGG
DraI 3889 TTTAAA TTT^AAA
3999 TTTAAA
Eco81I 1475 CCTCAGG CC^T[ACGT]AGG
2885 CCTGAGG
EcoO65I 491 GGTGACC G^GT[ACGT]ACC
1052 GGTGACC
EcoT22I 1409 ATGCAT ATGCA^T
3012 ATGCAT
Hin1I 1032 GGCGCC G[GA]^CG[CT]C
2800 GACGCC
KpnI 1194 GGTACC GGTAC^C
2436 GGTACC
MspA1I 1892 CAGCTG C[AC]G^C[GT]G
1921 CAGCTG
MvnI 600 CGCG CG^CG
683 CGCG
NcoI 1026 CCATGG C^CATGG
2484 CCATGG
NsiI 1409 ATGCAT ATGCA^T
3012 ATGCAT
PflMI 2641 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3087 CCATCCTCTGG
PpuMI 2343 GGGTCCC [GA]G^G[AT]CC[CT]
2688 GGGTCCT
PvuII 1892 CAGCTG CAG^CTG
1921 CAGCTG
SacI 2681 GAGCTC GAGCT^C
3073 GAGCTC
SapI 1829 GAAGAGC GCTCTTC(1/4)
3273 GAAGAGC
SexAI 1241 ACCAGGT A^CC[AT]GGT
2439 ACCTGGT
Van91I 2641 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3087 CCATCCTCTGG
XmnI 1742 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2489 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI