################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccIII AclI Aor51HI AscI AseI AsnI AspI AvrII BamHI BbvCI BlnI BsaBI BseAI BsiEI BsiWI BspDI BspEI BssSI Bst1107I BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV FseI HincII HindII HpaI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI PacI PflFI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SgrAI SmaI SnaBI SpeI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflII 3714 CTTAAG C^TTAAG AgeI 2730 ACCGGT A^CCGGT AhdI 926 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3288 GTGCAC G^TGCAC ApaI 1938 GGGCCC GGGCC^C ApaLI 3288 GTGCAC G^TGCAC AspEI 926 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1516 TGCGCA TGC^GCA BbeI 1032 GGCGCC GGCGC^C BbrPI 2835 CACGTG CAC^GTG BcgI 1253 CGAAATATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 566 GGATAC GTATCC(6/5) BclI 1453 TGATCA T^GATCA BfrI 3714 CTTAAG C^TTAAG BglII 2463 AGATCT A^GATCT BlpI 2329 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2329 GCTGAGC GC^T[ACGT]AGC BsaWI 2730 ACCGGT [AT]^CCGG[AT] Bsp1407I 2778 TGTACA T^GTACA BspHI 1698 TCATGA T^CATGA BspLU11I 2781 ACATGT A^CATGT BsrBI 1186 GAGCGG CCGCTC(-3/-3) BsrFI 2730 ACCGGT [GA]^CCGG[CT] BsrGI 2778 TGTACA T^GTACA BssHII 599 GCGCGC G^CGCGC BstBI 1251 TTCGAA TT^CGAA BstXI 2804 CCATAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2463 AGATCT [GA]^GATC[CT] CelII 2329 GCTGAGC GC^T[ACGT]AGC Cfr10I 2730 ACCGGT [GA]^CCGG[CT] DrdI 2543 GACTTTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 926 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3358 CCTTGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1453 TGATCA T^GATCA FspI 1516 TGCGCA TGC^GCA HaeII 1032 GGCGCC [GA]GCGC^[CT] HindIII 2015 AAGCTT A^AGCTT KasI 1032 GGCGCC G^GCGCC MflI 2463 AGATCT [GA]^GATC[CT] NarI 1032 GGCGCC GG^CGCC NspV 1251 TTCGAA TT^CGAA PinAI 2730 ACCGGT A^CCGGT PmaCI 2835 CACGTG CAC^GTG PmlI 2835 CACGTG CAC^GTG RcaI 1698 TCATGA T^CATGA SbfI 1651 CCTGCAGG CCTGCA^GG SfoI 1032 GGCGCC GGC^GCC SfuI 1251 TTCGAA TT^CGAA SphI 1407 GCATGC GCATG^C Sse8387I 1651 CCTGCAGG CCTGCA^GG SspBI 2778 TGTACA T^GTACA XhoII 2463 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1194 GGTACC G^GTACC 2436 GGTACC AccI 1586 GTAGAC GT^[AC][GT]AC 3555 GTAGAC AccII 600 CGCG CG^CG 683 CGCG AcsI 3520 AAATTT [GA]^AATT[CT] 3829 GAATTT AcyI 1032 GGCGCC G[GA]^CG[CT]C 2800 GACGCC AflIII 42 ACACGT A^C[GA][CT]GT 2781 ACATGT AlwI 2383 GATCC GGATC(4/5) 1978 GGATC AocI 1475 CCTCAGG CC^T[ACGT]AGG 2885 CCTGAGG ApoI 3520 AAATTT [GA]^AATT[CT] 3829 GAATTT Asp700I 1742 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2489 GAACACATTC Asp718I 1194 GGTACC G^GTACC 2436 GGTACC BbsI 1915 GAAGAC GAAGAC(2/6) 3161 GTCTTC BglI 2939 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3764 GCCTGTGTGGC BpuAI 1915 GAAGAC GAAGAC(2/6) 3161 GTCTTC BsaAI 43 CACGTA [CT]AC^GT[GA] 2835 CACGTG BsaHI 1032 GGCGCC G[GA]^CG[CT]C 2800 GACGCC BsmBI 1727 CGTCTC CGTCTC(1/5) 11 GAGACG BsmI 3010 GAATGC GAATGC(1/-1) 3461 GCATTC BsrDI 561 GCAATG GCAATG(2/0) 3915 GCAATG BstEII 491 GGTGACC G^GT[ACGT]ACC 1052 GGTGACC BstPI 491 GGTGACC G^GT[ACGT]ACC 1052 GGTGACC BstUI 600 CGCG CG^CG 683 CGCG Bsu36I 1475 CCTCAGG CC^T[ACGT]AGG 2885 CCTGAGG DraI 3889 TTTAAA TTT^AAA 3999 TTTAAA Eco81I 1475 CCTCAGG CC^T[ACGT]AGG 2885 CCTGAGG EcoO65I 491 GGTGACC G^GT[ACGT]ACC 1052 GGTGACC EcoT22I 1409 ATGCAT ATGCA^T 3012 ATGCAT Hin1I 1032 GGCGCC G[GA]^CG[CT]C 2800 GACGCC KpnI 1194 GGTACC GGTAC^C 2436 GGTACC MspA1I 1892 CAGCTG C[AC]G^C[GT]G 1921 CAGCTG MvnI 600 CGCG CG^CG 683 CGCG NcoI 1026 CCATGG C^CATGG 2484 CCATGG NsiI 1409 ATGCAT ATGCA^T 3012 ATGCAT PflMI 2641 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3087 CCATCCTCTGG PpuMI 2343 GGGTCCC [GA]G^G[AT]CC[CT] 2688 GGGTCCT PvuII 1892 CAGCTG CAG^CTG 1921 CAGCTG SacI 2681 GAGCTC GAGCT^C 3073 GAGCTC SapI 1829 GAAGAGC GCTCTTC(1/4) 3273 GAAGAGC SexAI 1241 ACCAGGT A^CC[AT]GGT 2439 ACCTGGT Van91I 2641 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3087 CCATCCTCTGG XmnI 1742 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2489 GAACACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI