################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII AccII AclI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AviII AvrII BbeI BcgI BclI BglI BglII BlnI BsiEI BsiWI BspDI BspLU11I BssHII BstAPI BstUI Bsu36I CfoI ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KspI MluI MvnI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SmaI SphI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 69 GGTACC G^GTACC AccIII 2275 TCCGGA T^CCGGA AflII 1475 CTTAAG C^TTAAG Asp718I 69 GGTACC G^GTACC AspHI 1515 GAGCTC G[AT]GC[AT]^C AspI 1046 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2415 CCCGAG C^[CT]CG[GA]G BbrPI 669 CACGTG CAC^GTG BbvCI 2186 GCTGAGG CCTCAGC(-5/-2) BciVI 1721 GTATCC GTATCC(6/5) BfrI 1475 CTTAAG C^TTAAG BlpI 2324 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2324 GCTGAGC GC^T[ACGT]AGC BseAI 2275 TCCGGA T^CCGGA BsiHKAI 1515 GAGCTC G[AT]GC[AT]^C BsmI 1601 GAATGC GAATGC(1/-1) BsoBI 2415 CCCGAG C^[CT]CG[GA]G BspEI 2275 TCCGGA T^CCGGA BspHI 3216 TCATGA T^CATGA BsrBI 1899 GAGCGG CCGCTC(-3/-3) BsrFI 13 GCCGGC [GA]^CCGG[CT] BssSI 1347 CTCGTG CACGAG(-5/-1) Bst1107I 2716 GTATAC GTA^TAC BstBI 2874 TTCGAA TT^CGAA BstXI 2920 CCAGACAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2716 GTATAC GTA^TAC CelII 2324 GCTGAGC GC^T[ACGT]AGC Cfr10I 13 GCCGGC [GA]^CCGG[CT] EcoRV 1089 GATATC GAT^ATC KpnI 69 GGTACC GGTAC^C MroI 2275 TCCGGA T^CCGGA NaeI 13 GCCGGC GCC^GGC NgoMIV 13 GCCGGC G^CCGGC NspI 3367 GCATGT [GA]CATG^[CT] NspV 2874 TTCGAA TT^CGAA PflFI 1046 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3647 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 669 CACGTG CAC^GTG PmlI 669 CACGTG CAC^GTG PshAI 1453 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 607 CTGCAG CTGCA^G PvuII 1428 CAGCTG CAG^CTG RcaI 3216 TCATGA T^CATGA SacI 1515 GAGCTC GAGCT^C SapI 544 GAAGAGC GCTCTTC(1/4) SfuI 2874 TTCGAA TT^CGAA SgrAI 12 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 2719 TACGTA TAC^GTA SpeI 4029 ACTAGT A^CTAGT StuI 41 AGGCCT AGG^CCT Tth111I 1046 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3647 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1298 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 10 GACGCC G[GA]^CG[CT]C 1211 GGCGTC Asp700I 48 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2261 GAAACTCTTC BsaAI 669 CACGTG [CT]AC^GT[GA] 2719 TACGTA BsaBI 422 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3140 GATGAAGATC BsaHI 10 GACGCC G[GA]^CG[CT]C 1211 GGCGTC BsaWI 349 TCCGGT [AT]^CCGG[AT] 2275 TCCGGA EcoNI 1005 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1441 CCTGTGGTAGG Hin1I 10 GACGCC G[GA]^CG[CT]C 1211 GGCGTC HincII 133 GTTGAC GT[CT]^[GA]AC 3760 GTTGAC HindII 133 GTTGAC GT[CT]^[GA]AC 3760 GTTGAC MamI 422 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3140 GATGAAGATC MfeI 565 CAATTG C^AATTG 2925 CAATTG MspA1I 189 CAGCGG C[AC]G^C[GT]G 1428 CAGCTG MunI 565 CAATTG C^AATTG 2925 CAATTG NcoI 218 CCATGG C^CATGG 3492 CCATGG XcmI 473 CCAAGAAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3126 CCAACGATGAGGTGG XmnI 48 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2261 GAAACTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI AspEI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI Eam1105I EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII