##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AclI AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AviII AvrII
BbeI BcgI BclI BglI BglII
BlnI BsiEI BsiWI BspDI BspLU11I
BssHII BstAPI BstUI Bsu36I CfoI
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HpaI KasI KspI
MluI MvnI NarI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SmaI SphI
Sse8387I SspI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 69 GGTACC G^GTACC
AccIII 2275 TCCGGA T^CCGGA
AflII 1475 CTTAAG C^TTAAG
Asp718I 69 GGTACC G^GTACC
AspHI 1515 GAGCTC G[AT]GC[AT]^C
AspI 1046 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2415 CCCGAG C^[CT]CG[GA]G
BbrPI 669 CACGTG CAC^GTG
BbvCI 2186 GCTGAGG CCTCAGC(-5/-2)
BciVI 1721 GTATCC GTATCC(6/5)
BfrI 1475 CTTAAG C^TTAAG
BlpI 2324 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2324 GCTGAGC GC^T[ACGT]AGC
BseAI 2275 TCCGGA T^CCGGA
BsiHKAI 1515 GAGCTC G[AT]GC[AT]^C
BsmI 1601 GAATGC GAATGC(1/-1)
BsoBI 2415 CCCGAG C^[CT]CG[GA]G
BspEI 2275 TCCGGA T^CCGGA
BspHI 3216 TCATGA T^CATGA
BsrBI 1899 GAGCGG CCGCTC(-3/-3)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BssSI 1347 CTCGTG CACGAG(-5/-1)
Bst1107I 2716 GTATAC GTA^TAC
BstBI 2874 TTCGAA TT^CGAA
BstXI 2920 CCAGACAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2716 GTATAC GTA^TAC
CelII 2324 GCTGAGC GC^T[ACGT]AGC
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
EcoRV 1089 GATATC GAT^ATC
KpnI 69 GGTACC GGTAC^C
MroI 2275 TCCGGA T^CCGGA
NaeI 13 GCCGGC GCC^GGC
NgoMIV 13 GCCGGC G^CCGGC
NspI 3367 GCATGT [GA]CATG^[CT]
NspV 2874 TTCGAA TT^CGAA
PflFI 1046 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3647 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 669 CACGTG CAC^GTG
PmlI 669 CACGTG CAC^GTG
PshAI 1453 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 607 CTGCAG CTGCA^G
PvuII 1428 CAGCTG CAG^CTG
RcaI 3216 TCATGA T^CATGA
SacI 1515 GAGCTC GAGCT^C
SapI 544 GAAGAGC GCTCTTC(1/4)
SfuI 2874 TTCGAA TT^CGAA
SgrAI 12 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 2719 TACGTA TAC^GTA
SpeI 4029 ACTAGT A^CTAGT
StuI 41 AGGCCT AGG^CCT
Tth111I 1046 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3647 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1298 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 10 GACGCC G[GA]^CG[CT]C
1211 GGCGTC
Asp700I 48 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2261 GAAACTCTTC
BsaAI 669 CACGTG [CT]AC^GT[GA]
2719 TACGTA
BsaBI 422 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3140 GATGAAGATC
BsaHI 10 GACGCC G[GA]^CG[CT]C
1211 GGCGTC
BsaWI 349 TCCGGT [AT]^CCGG[AT]
2275 TCCGGA
EcoNI 1005 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1441 CCTGTGGTAGG
Hin1I 10 GACGCC G[GA]^CG[CT]C
1211 GGCGTC
HincII 133 GTTGAC GT[CT]^[GA]AC
3760 GTTGAC
HindII 133 GTTGAC GT[CT]^[GA]AC
3760 GTTGAC
MamI 422 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3140 GATGAAGATC
MfeI 565 CAATTG C^AATTG
2925 CAATTG
MspA1I 189 CAGCGG C[AC]G^C[GT]G
1428 CAGCTG
MunI 565 CAATTG C^AATTG
2925 CAATTG
NcoI 218 CCATGG C^CATGG
3492 CCATGG
XcmI 473 CCAAGAAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3126 CCAACGATGAGGTGG
XmnI 48 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2261 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AspEI
AvaII BalI BamHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstYI Cac8I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI Eam1105I EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII