##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccII       AclI        AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AviII       AvrII       
BbeI        BcgI        BclI        BglI        BglII       
BlnI        BsiEI       BsiWI       BspDI       BspLU11I    
BssHII      BstAPI      BstUI       Bsu36I      CfoI        
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        HinP1I      HpaI        KasI        KspI        
MluI        MvnI        NarI        NdeI        NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SmaI        SphI        
Sse8387I    SspI        SwaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I         69  GGTACC               G^GTACC
AccIII       2275  TCCGGA               T^CCGGA
AflII        1475  CTTAAG               C^TTAAG
Asp718I        69  GGTACC               G^GTACC
AspHI        1515  GAGCTC               G[AT]GC[AT]^C
AspI         1046  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2415  CCCGAG               C^[CT]CG[GA]G
BbrPI         669  CACGTG               CAC^GTG
BbvCI        2186  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1721  GTATCC               GTATCC(6/5)
BfrI         1475  CTTAAG               C^TTAAG
BlpI         2324  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2324  GCTGAGC              GC^T[ACGT]AGC
BseAI        2275  TCCGGA               T^CCGGA
BsiHKAI      1515  GAGCTC               G[AT]GC[AT]^C
BsmI         1601  GAATGC               GAATGC(1/-1)
BsoBI        2415  CCCGAG               C^[CT]CG[GA]G
BspEI        2275  TCCGGA               T^CCGGA
BspHI        3216  TCATGA               T^CATGA
BsrBI        1899  GAGCGG               CCGCTC(-3/-3)
BsrFI          13  GCCGGC               [GA]^CCGG[CT]
BssSI        1347  CTCGTG               CACGAG(-5/-1)
Bst1107I     2716  GTATAC               GTA^TAC
BstBI        2874  TTCGAA               TT^CGAA
BstXI        2920  CCAGACAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2716  GTATAC               GTA^TAC
CelII        2324  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         13  GCCGGC               [GA]^CCGG[CT]
EcoRV        1089  GATATC               GAT^ATC
KpnI           69  GGTACC               GGTAC^C
MroI         2275  TCCGGA               T^CCGGA
NaeI           13  GCCGGC               GCC^GGC
NgoMIV         13  GCCGGC               G^CCGGC
NspI         3367  GCATGT               [GA]CATG^[CT]
NspV         2874  TTCGAA               TT^CGAA
PflFI        1046  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3647  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         669  CACGTG               CAC^GTG
PmlI          669  CACGTG               CAC^GTG
PshAI        1453  GACCCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          607  CTGCAG               CTGCA^G
PvuII        1428  CAGCTG               CAG^CTG
RcaI         3216  TCATGA               T^CATGA
SacI         1515  GAGCTC               GAGCT^C
SapI          544  GAAGAGC              GCTCTTC(1/4)
SfuI         2874  TTCGAA               TT^CGAA
SgrAI          12  CGCCGGCG             C[GA]^CCGG[CT]G
SnaBI        2719  TACGTA               TAC^GTA
SpeI         4029  ACTAGT               A^CTAGT
StuI           41  AGGCCT               AGG^CCT
Tth111I      1046  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3647  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1298  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI           10  GACGCC               G[GA]^CG[CT]C
             1211  GGCGTC              
Asp700I        48  GAAGCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2261  GAAACTCTTC          
BsaAI         669  CACGTG               [CT]AC^GT[GA]
             2719  TACGTA              
BsaBI         422  GATTGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3140  GATGAAGATC          
BsaHI          10  GACGCC               G[GA]^CG[CT]C
             1211  GGCGTC              
BsaWI         349  TCCGGT               [AT]^CCGG[AT]
             2275  TCCGGA              
EcoNI        1005  CCTGAACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1441  CCTGTGGTAGG         
Hin1I          10  GACGCC               G[GA]^CG[CT]C
             1211  GGCGTC              
HincII        133  GTTGAC               GT[CT]^[GA]AC
             3760  GTTGAC              
HindII        133  GTTGAC               GT[CT]^[GA]AC
             3760  GTTGAC              
MamI          422  GATTGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3140  GATGAAGATC          
MfeI          565  CAATTG               C^AATTG
             2925  CAATTG              
MspA1I        189  CAGCGG               C[AC]G^C[GT]G
             1428  CAGCTG              
MunI          565  CAATTG               C^AATTG
             2925  CAATTG              
NcoI          218  CCATGG               C^CATGG
             3492  CCATGG              
XcmI          473  CCAAGAAACTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3126  CCAACGATGAGGTGG     
XmnI           48  GAAGCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2261  GAAACTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApoI        AspEI       
AvaII       BalI        BamHI       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    Bsp1407I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        Eam1105I    EarI        EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII