##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AclI AflII AhdI Aor51HI
AscI AspEI BamHI BbrPI BcgI
BfrI BglII BsaAI BsaBI BseAI
BsiWI BspEI BssHII BstBI CpoI
Eam1105I Eco47III EcoRV FseI HindIII
KspI MamI MluI MroI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2637 GACGTC GACGT^C
Acc65I 1297 GGTACC G^GTACC
AocI 2628 CCTGAGG CC^T[ACGT]AGG
Asp700I 3982 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1297 GGTACC G^GTACC
AvaI 1942 CCCGGG C^[CT]CG[GA]G
AviII 2005 TGCGCA TGC^GCA
AvrII 2697 CCTAGG C^CTAGG
BbeI 2270 GGCGCC GGCGC^C
BglI 2735 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2697 CCTAGG C^CTAGG
BsoBI 1942 CCCGGG C^[CT]CG[GA]G
BspDI 1128 ATCGAT AT^CGAT
BspHI 3314 TCATGA T^CATGA
BsrBI 2274 CCGCTC CCGCTC(-3/-3)
BssSI 2414 CACGAG CACGAG(-5/-1)
Bst1107I 2885 GTATAC GTA^TAC
BstAPI 874 GCACAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 625 CCATAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2885 GTATAC GTA^TAC
Bsu36I 2628 CCTGAGG CC^T[ACGT]AGG
ClaI 1128 ATCGAT AT^CGAT
DrdI 2343 GACAGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2365 CGGCCG C^GGCCG
EclXI 2365 CGGCCG C^GGCCG
Eco52I 2365 CGGCCG C^GGCCG
Eco81I 2628 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2223 ATGCAT ATGCA^T
FspI 2005 TGCGCA TGC^GCA
HaeII 2270 GGCGCC [GA]GCGC^[CT]
HpaI 1599 GTTAAC GTT^AAC
KasI 2270 GGCGCC G^GCGCC
KpnI 1297 GGTACC GGTAC^C
MfeI 250 CAATTG C^AATTG
MunI 250 CAATTG C^AATTG
NarI 2270 GGCGCC GG^CGCC
NdeI 1397 CATATG CA^TATG
NsiI 2223 ATGCAT ATGCA^T
PflMI 2132 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2473 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1610 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3314 TCATGA T^CATGA
SfoI 2270 GGCGCC GGC^GCC
SmaI 1942 CCCGGG CCC^GGG
SwaI 3564 ATTTAAAT ATTT^AAAT
Van91I 2132 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1942 CCCGGG C^CCGGG
XmaI 1942 CCCGGG C^CCGGG
XmnI 3982 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 53 enzymes) #
###################################
AflIII 2557 ACATGT A^C[GA][CT]GT
3859 ACATGT
AgeI 2162 ACCGGT A^CCGGT
3047 ACCGGT
Alw44I 951 GTGCAC G^TGCAC
2100 GTGCAC
AlwNI 198 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1724 CAGACACTG
ApaLI 951 GTGCAC G^TGCAC
2100 GTGCAC
AseI 2716 ATTAAT AT^TAAT
3829 ATTAAT
AsnI 2716 ATTAAT AT^TAAT
3829 ATTAAT
AspI 2050 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3526 GACGGCGTC
BalI 91 TGGCCA TGG^CCA
562 TGGCCA
BbsI 2771 GAAGAC GAAGAC(2/6)
3286 GTCTTC
BbvCI 449 CCTCAGC CCTCAGC(-5/-2)
282 GCTGAGG
BciVI 350 GGATAC GTATCC(6/5)
1961 GGATAC
BclI 1456 TGATCA T^GATCA
2083 TGATCA
BlpI 105 GCTAAGC GC^T[ACGT]AGC
189 GCTCAGC
Bpu1102I 105 GCTAAGC GC^T[ACGT]AGC
189 GCTCAGC
BpuAI 2771 GAAGAC GAAGAC(2/6)
3286 GTCTTC
BsaI 2982 GAGACC GGTCTC(1/5)
407 GGTCTC
BsiEI 1753 CGACCG CG[GA][CT]^CG
2365 CGGCCG
BsmI 77 GAATGC GAATGC(1/-1)
369 GCATTC
Bsp1407I 2410 TGTACA T^GTACA
3297 TGTACA
BspLU11I 2557 ACATGT A^CATGT
3859 ACATGT
BsrDI 665 CATTGC GCAATG(2/0)
1100 CATTGC
BsrFI 2162 ACCGGT [GA]^CCGG[CT]
3047 ACCGGT
BsrGI 2410 TGTACA T^GTACA
3297 TGTACA
BstEII 3489 GGTGACC G^GT[ACGT]ACC
3702 GGTCACC
BstPI 3489 GGTGACC G^GT[ACGT]ACC
3702 GGTCACC
BstYI 731 AGATCC [GA]^GATC[CT]
3120 AGATCC
CelII 105 GCTAAGC GC^T[ACGT]AGC
189 GCTCAGC
Cfr10I 2162 ACCGGT [GA]^CCGG[CT]
3047 ACCGGT
DsaI 1957 CCATGG C^C[GA][CT]GG
2791 CCATGG
EcoO65I 3489 GGTGACC G^GT[ACGT]ACC
3702 GGTCACC
EcoRI 311 GAATTC G^AATTC
2692 GAATTC
FauI 1763 CCCGC CCCGC(4/6)
1792 CCCGC
FbaI 1456 TGATCA T^GATCA
2083 TGATCA
HincII 855 GTTGAC GT[CT]^[GA]AC
1599 GTTAAC
HindII 855 GTTGAC GT[CT]^[GA]AC
1599 GTTAAC
MflI 731 AGATCC [GA]^GATC[CT]
3120 AGATCC
MluNI 91 TGGCCA TGG^CCA
562 TGGCCA
MscI 91 TGGCCA TGG^CCA
562 TGGCCA
NcoI 1957 CCATGG C^CATGG
2791 CCATGG
PflFI 2050 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3526 GACGGCGTC
PinAI 2162 ACCGGT A^CCGGT
3047 ACCGGT
PshBI 2716 ATTAAT AT^TAAT
3829 ATTAAT
PstI 195 CTGCAG CTGCA^G
2747 CTGCAG
SapI 1683 GCTCTTC GCTCTTC(1/4)
3886 GCTCTTC
SexAI 1575 ACCTGGT A^CC[AT]GGT
2930 ACCAGGT
SmlI 2145 CTCAAG C^T[CT][GA]AG
3660 CTTGAG
SphI 1152 GCATGC GCATG^C
2419 GCATGC
SspBI 2410 TGTACA T^GTACA
3297 TGTACA
StuI 3083 AGGCCT AGG^CCT
3272 AGGCCT
Tth111I 2050 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3526 GACGGCGTC
XcmI 337 CCACAATGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3802 CCATATTCCTTCTGG
XhoII 731 AGATCC [GA]^GATC[CT]
3120 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApaI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I