################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AccIII AclI AflII AhdI Aor51HI AscI AspEI BamHI BbrPI BcgI BfrI BglII BsaAI BsaBI BseAI BsiWI BspEI BssHII BstBI CpoI Eam1105I Eco47III EcoRV FseI HindIII KspI MamI MluI MroI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 2637 GACGTC GACGT^C Acc65I 1297 GGTACC G^GTACC AocI 2628 CCTGAGG CC^T[ACGT]AGG Asp700I 3982 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1297 GGTACC G^GTACC AvaI 1942 CCCGGG C^[CT]CG[GA]G AviII 2005 TGCGCA TGC^GCA AvrII 2697 CCTAGG C^CTAGG BbeI 2270 GGCGCC GGCGC^C BglI 2735 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2697 CCTAGG C^CTAGG BsoBI 1942 CCCGGG C^[CT]CG[GA]G BspDI 1128 ATCGAT AT^CGAT BspHI 3314 TCATGA T^CATGA BsrBI 2274 CCGCTC CCGCTC(-3/-3) BssSI 2414 CACGAG CACGAG(-5/-1) Bst1107I 2885 GTATAC GTA^TAC BstAPI 874 GCACAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 625 CCATAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2885 GTATAC GTA^TAC Bsu36I 2628 CCTGAGG CC^T[ACGT]AGG ClaI 1128 ATCGAT AT^CGAT DrdI 2343 GACAGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2365 CGGCCG C^GGCCG EclXI 2365 CGGCCG C^GGCCG Eco52I 2365 CGGCCG C^GGCCG Eco81I 2628 CCTGAGG CC^T[ACGT]AGG EcoT22I 2223 ATGCAT ATGCA^T FspI 2005 TGCGCA TGC^GCA HaeII 2270 GGCGCC [GA]GCGC^[CT] HpaI 1599 GTTAAC GTT^AAC KasI 2270 GGCGCC G^GCGCC KpnI 1297 GGTACC GGTAC^C MfeI 250 CAATTG C^AATTG MunI 250 CAATTG C^AATTG NarI 2270 GGCGCC GG^CGCC NdeI 1397 CATATG CA^TATG NsiI 2223 ATGCAT ATGCA^T PflMI 2132 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2473 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1610 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3314 TCATGA T^CATGA SfoI 2270 GGCGCC GGC^GCC SmaI 1942 CCCGGG CCC^GGG SwaI 3564 ATTTAAAT ATTT^AAAT Van91I 2132 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1942 CCCGGG C^CCGGG XmaI 1942 CCCGGG C^CCGGG XmnI 3982 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 53 enzymes) # ################################### AflIII 2557 ACATGT A^C[GA][CT]GT 3859 ACATGT AgeI 2162 ACCGGT A^CCGGT 3047 ACCGGT Alw44I 951 GTGCAC G^TGCAC 2100 GTGCAC AlwNI 198 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1724 CAGACACTG ApaLI 951 GTGCAC G^TGCAC 2100 GTGCAC AseI 2716 ATTAAT AT^TAAT 3829 ATTAAT AsnI 2716 ATTAAT AT^TAAT 3829 ATTAAT AspI 2050 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3526 GACGGCGTC BalI 91 TGGCCA TGG^CCA 562 TGGCCA BbsI 2771 GAAGAC GAAGAC(2/6) 3286 GTCTTC BbvCI 449 CCTCAGC CCTCAGC(-5/-2) 282 GCTGAGG BciVI 350 GGATAC GTATCC(6/5) 1961 GGATAC BclI 1456 TGATCA T^GATCA 2083 TGATCA BlpI 105 GCTAAGC GC^T[ACGT]AGC 189 GCTCAGC Bpu1102I 105 GCTAAGC GC^T[ACGT]AGC 189 GCTCAGC BpuAI 2771 GAAGAC GAAGAC(2/6) 3286 GTCTTC BsaI 2982 GAGACC GGTCTC(1/5) 407 GGTCTC BsiEI 1753 CGACCG CG[GA][CT]^CG 2365 CGGCCG BsmI 77 GAATGC GAATGC(1/-1) 369 GCATTC Bsp1407I 2410 TGTACA T^GTACA 3297 TGTACA BspLU11I 2557 ACATGT A^CATGT 3859 ACATGT BsrDI 665 CATTGC GCAATG(2/0) 1100 CATTGC BsrFI 2162 ACCGGT [GA]^CCGG[CT] 3047 ACCGGT BsrGI 2410 TGTACA T^GTACA 3297 TGTACA BstEII 3489 GGTGACC G^GT[ACGT]ACC 3702 GGTCACC BstPI 3489 GGTGACC G^GT[ACGT]ACC 3702 GGTCACC BstYI 731 AGATCC [GA]^GATC[CT] 3120 AGATCC CelII 105 GCTAAGC GC^T[ACGT]AGC 189 GCTCAGC Cfr10I 2162 ACCGGT [GA]^CCGG[CT] 3047 ACCGGT DsaI 1957 CCATGG C^C[GA][CT]GG 2791 CCATGG EcoO65I 3489 GGTGACC G^GT[ACGT]ACC 3702 GGTCACC EcoRI 311 GAATTC G^AATTC 2692 GAATTC FauI 1763 CCCGC CCCGC(4/6) 1792 CCCGC FbaI 1456 TGATCA T^GATCA 2083 TGATCA HincII 855 GTTGAC GT[CT]^[GA]AC 1599 GTTAAC HindII 855 GTTGAC GT[CT]^[GA]AC 1599 GTTAAC MflI 731 AGATCC [GA]^GATC[CT] 3120 AGATCC MluNI 91 TGGCCA TGG^CCA 562 TGGCCA MscI 91 TGGCCA TGG^CCA 562 TGGCCA NcoI 1957 CCATGG C^CATGG 2791 CCATGG PflFI 2050 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3526 GACGGCGTC PinAI 2162 ACCGGT A^CCGGT 3047 ACCGGT PshBI 2716 ATTAAT AT^TAAT 3829 ATTAAT PstI 195 CTGCAG CTGCA^G 2747 CTGCAG SapI 1683 GCTCTTC GCTCTTC(1/4) 3886 GCTCTTC SexAI 1575 ACCTGGT A^CC[AT]GGT 2930 ACCAGGT SmlI 2145 CTCAAG C^T[CT][GA]AG 3660 CTTGAG SphI 1152 GCATGC GCATG^C 2419 GCATGC SspBI 2410 TGTACA T^GTACA 3297 TGTACA StuI 3083 AGGCCT AGG^CCT 3272 AGGCCT Tth111I 2050 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3526 GACGGCGTC XcmI 337 CCACAATGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3802 CCATATTCCTTCTGG XhoII 731 AGATCC [GA]^GATC[CT] 3120 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI ApaI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I