##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AgeI Alw44I AocI ApaLI
AscI AseI AsnI AspI BbrPI
BciVI BsaBI BsiWI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CpoI Eco81I EcoO65I EcoRV
EcoT22I FseI HincII HindII HpaI
MamI MluI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI SexAI SfuI SgrAI
SnaBI Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 325 GACGTC GACGT^C
Acc65I 312 GGTACC G^GTACC
AccIII 2957 TCCGGA T^CCGGA
AflII 463 CTTAAG C^TTAAG
AhdI 1757 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1863 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 312 GGTACC G^GTACC
AspEI 1757 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4165 CCTAGG C^CTAGG
BclI 731 TGATCA T^GATCA
BfrI 463 CTTAAG C^TTAAG
BglII 1001 AGATCT A^GATCT
BlnI 4165 CCTAGG C^CTAGG
BseAI 2957 TCCGGA T^CCGGA
BsiEI 4229 CGGCCG CG[GA][CT]^CG
BsmBI 3047 CGTCTC CGTCTC(1/5)
Bsp1407I 281 TGTACA T^GTACA
BspDI 24 ATCGAT AT^CGAT
BspEI 2957 TCCGGA T^CCGGA
BspHI 734 TCATGA T^CATGA
BspLU11I 2771 ACATGT A^CATGT
BsrDI 516 CATTGC GCAATG(2/0)
BsrGI 281 TGTACA T^GTACA
BssHII 4340 GCGCGC G^CGCGC
ClaI 24 ATCGAT AT^CGAT
DraIII 3037 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 582 GACACAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4229 CGGCCG C^GGCCG
Eam1105I 1757 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4229 CGGCCG C^GGCCG
Eco52I 4229 CGGCCG C^GGCCG
FbaI 731 TGATCA T^GATCA
KpnI 312 GGTACC GGTAC^C
MfeI 2702 CAATTG C^AATTG
MroI 2957 TCCGGA T^CCGGA
MunI 2702 CAATTG C^AATTG
PaeR7I 3698 CTCGAG C^TCGAG
RcaI 734 TCATGA T^CATGA
ScaI 2588 AGTACT AGT^ACT
SpeI 5135 ACTAGT A^CTAGT
SphI 3509 GCATGC GCATG^C
SspBI 281 TGTACA T^GTACA
XhoI 3698 CTCGAG C^TCGAG
XmnI 1863 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 157 GAATTC [GA]^AATT[CT]
987 GAATTC
ApaI 4302 GGGCCC GGGCC^C
4618 GGGCCC
ApoI 157 GAATTC [GA]^AATT[CT]
987 GAATTC
AviII 1229 TGCGCA TGC^GCA
4694 TGCGCA
BbsI 3807 GAAGAC GAAGAC(2/6)
4150 GAAGAC
BpuAI 3807 GAAGAC GAAGAC(2/6)
4150 GAAGAC
BsaAI 980 TACGTG [CT]AC^GT[GA]
3339 CACGTA
BsaWI 309 TCCGGT [AT]^CCGG[AT]
2957 TCCGGA
BsmI 1026 GAATGC GAATGC(1/-1)
1218 GAATGC
DraI 4855 TTTAAA TTT^AAA
4938 TTTAAA
EcoRI 157 GAATTC G^AATTC
987 GAATTC
FspI 1229 TGCGCA TGC^GCA
4694 TGCGCA
HindIII 3475 AAGCTT A^AGCTT
4820 AAGCTT
KspI 4226 CCGCGG CCGC^GG
4406 CCGCGG
MslI 870 CATAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2955 CATCCGGATG
SacII 4226 CCGCGG CCGC^GG
4406 CCGCGG
SfiI 4272 GGCCACCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4572 GGCCTCGCCGGCC
SspI 35 AATATT AAT^ATT
5152 AATATT
StuI 4366 AGGCCT AGG^CCT
4680 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI