################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AclI AgeI Alw44I AocI ApaLI AscI AseI AsnI AspI BbrPI BciVI BsaBI BsiWI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CpoI Eco81I EcoO65I EcoRV EcoT22I FseI HincII HindII HpaI MamI MluI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 325 GACGTC GACGT^C Acc65I 312 GGTACC G^GTACC AccIII 2957 TCCGGA T^CCGGA AflII 463 CTTAAG C^TTAAG AhdI 1757 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1863 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 312 GGTACC G^GTACC AspEI 1757 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 4165 CCTAGG C^CTAGG BclI 731 TGATCA T^GATCA BfrI 463 CTTAAG C^TTAAG BglII 1001 AGATCT A^GATCT BlnI 4165 CCTAGG C^CTAGG BseAI 2957 TCCGGA T^CCGGA BsiEI 4229 CGGCCG CG[GA][CT]^CG BsmBI 3047 CGTCTC CGTCTC(1/5) Bsp1407I 281 TGTACA T^GTACA BspDI 24 ATCGAT AT^CGAT BspEI 2957 TCCGGA T^CCGGA BspHI 734 TCATGA T^CATGA BspLU11I 2771 ACATGT A^CATGT BsrDI 516 CATTGC GCAATG(2/0) BsrGI 281 TGTACA T^GTACA BssHII 4340 GCGCGC G^CGCGC ClaI 24 ATCGAT AT^CGAT DraIII 3037 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 582 GACACAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4229 CGGCCG C^GGCCG Eam1105I 1757 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 4229 CGGCCG C^GGCCG Eco52I 4229 CGGCCG C^GGCCG FbaI 731 TGATCA T^GATCA KpnI 312 GGTACC GGTAC^C MfeI 2702 CAATTG C^AATTG MroI 2957 TCCGGA T^CCGGA MunI 2702 CAATTG C^AATTG PaeR7I 3698 CTCGAG C^TCGAG RcaI 734 TCATGA T^CATGA ScaI 2588 AGTACT AGT^ACT SpeI 5135 ACTAGT A^CTAGT SphI 3509 GCATGC GCATG^C SspBI 281 TGTACA T^GTACA XhoI 3698 CTCGAG C^TCGAG XmnI 1863 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 157 GAATTC [GA]^AATT[CT] 987 GAATTC ApaI 4302 GGGCCC GGGCC^C 4618 GGGCCC ApoI 157 GAATTC [GA]^AATT[CT] 987 GAATTC AviII 1229 TGCGCA TGC^GCA 4694 TGCGCA BbsI 3807 GAAGAC GAAGAC(2/6) 4150 GAAGAC BpuAI 3807 GAAGAC GAAGAC(2/6) 4150 GAAGAC BsaAI 980 TACGTG [CT]AC^GT[GA] 3339 CACGTA BsaWI 309 TCCGGT [AT]^CCGG[AT] 2957 TCCGGA BsmI 1026 GAATGC GAATGC(1/-1) 1218 GAATGC DraI 4855 TTTAAA TTT^AAA 4938 TTTAAA EcoRI 157 GAATTC G^AATTC 987 GAATTC FspI 1229 TGCGCA TGC^GCA 4694 TGCGCA HindIII 3475 AAGCTT A^AGCTT 4820 AAGCTT KspI 4226 CCGCGG CCGC^GG 4406 CCGCGG MslI 870 CATAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2955 CATCCGGATG SacII 4226 CCGCGG CCGC^GG 4406 CCGCGG SfiI 4272 GGCCACCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4572 GGCCTCGCCGGCC SspI 35 AATATT AAT^ATT 5152 AATATT StuI 4366 AGGCCT AGG^CCT 4680 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI Aor51HI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI