##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccII AflII AflIII AgeI
AhdI ApaI AscI AspEI AviII
AvrII BamHI BbrPI BfrI BlnI
BsaBI BsiWI BsmBI BspDI BspLU11I
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
FauI FseI FspI HpaI KspI
MamI MluI MvnI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII ScaI SfiI SfuI SgrAI
SmaI SphI StuI SwaI XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 2488 TCCGGA T^CCGGA
AclI 1035 AACGTT AA^CGTT
Alw44I 2269 GTGCAC G^TGCAC
Aor51HI 753 AGCGCT AGC^GCT
ApaLI 2269 GTGCAC G^TGCAC
AseI 1380 ATTAAT AT^TAAT
AsnI 1380 ATTAAT AT^TAAT
AspI 229 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 335 GGCGCC GGCGC^C
BbsI 829 GTCTTC GAAGAC(2/6)
BcgI 3899 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2668 GTATCC GTATCC(6/5)
BglI 3897 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4147 AGATCT A^GATCT
BlpI 3954 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3954 GCTGAGC GC^T[ACGT]AGC
BpuAI 829 GTCTTC GAAGAC(2/6)
BsaI 3501 GGTCTC GGTCTC(1/5)
BseAI 2488 TCCGGA T^CCGGA
BsiEI 2545 CGGTCG CG[GA][CT]^CG
BspEI 2488 TCCGGA T^CCGGA
BspHI 542 TCATGA T^CATGA
BssSI 25 CTCGTG CACGAG(-5/-1)
CelII 3954 GCTGAGC GC^T[ACGT]AGC
Eco47III 753 AGCGCT AGC^GCT
EcoNI 839 CCTAATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1665 ATGCAT ATGCA^T
HindIII 2586 AAGCTT A^AGCTT
KasI 335 GGCGCC G^GCGCC
MfeI 1356 CAATTG C^AATTG
MroI 2488 TCCGGA T^CCGGA
MunI 1356 CAATTG C^AATTG
NaeI 1946 GCCGGC GCC^GGC
NarI 335 GGCGCC GG^CGCC
NgoMIV 1946 GCCGGC G^CCGGC
NheI 1643 GCTAGC G^CTAGC
NsiI 1665 ATGCAT ATGCA^T
PflFI 229 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 189 CCAGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3726 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1380 ATTAAT AT^TAAT
Psp1406I 1035 AACGTT AA^CGTT
RcaI 542 TCATGA T^CATGA
SbfI 3737 CCTGCAGG CCTGCA^GG
SexAI 2089 ACCTGGT A^CC[AT]GGT
SfoI 335 GGCGCC GGC^GCC
SnaBI 3770 TACGTA TAC^GTA
SpeI 3966 ACTAGT A^CTAGT
Sse8387I 3737 CCTGCAGG CCTGCA^GG
Tth111I 229 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 189 CCAGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 785 GGTACC G^GTACC
2124 GGTACC
AcyI 335 GGCGCC G[GA]^CG[CT]C
3157 GGCGTC
AocI 2165 CCTCAGG CC^T[ACGT]AGG
3787 CCTCAGG
Asp700I 568 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2944 GAATGAGTTC
Asp718I 785 GGTACC G^GTACC
2124 GGTACC
AvaI 3288 CCCGAG C^[CT]CG[GA]G
4095 CCCGAG
BbvCI 4019 CCTCAGC CCTCAGC(-5/-2)
4086 CCTCAGC
BsaAI 1717 CACGTA [CT]AC^GT[GA]
3770 TACGTA
BsaHI 335 GGCGCC G[GA]^CG[CT]C
3157 GGCGTC
BsoBI 3288 CCCGAG C^[CT]CG[GA]G
4095 CCCGAG
Bsp1407I 494 TGTACA T^GTACA
2695 TGTACA
BsrBI 525 CCGCTC CCGCTC(-3/-3)
4062 GAGCGG
BsrDI 1228 CATTGC GCAATG(2/0)
2239 GCAATG
BsrFI 1946 GCCGGC [GA]^CCGG[CT]
3322 ACCGGC
BsrGI 494 TGTACA T^GTACA
2695 TGTACA
BstXI 683 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3392 CCAAGTCAATGG
BstYI 3517 GGATCT [GA]^GATC[CT]
4147 AGATCT
Bsu36I 2165 CCTCAGG CC^T[ACGT]AGG
3787 CCTCAGG
Cfr10I 1946 GCCGGC [GA]^CCGG[CT]
3322 ACCGGC
DraI 164 TTTAAA TTT^AAA
2283 TTTAAA
DrdI 1062 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2245 GACCACTGGGTC
Eco81I 2165 CCTCAGG CC^T[ACGT]AGG
3787 CCTCAGG
EcoRI 1217 GAATTC G^AATTC
4125 GAATTC
EcoRV 1147 GATATC GAT^ATC
4335 GATATC
HgaI 2814 GCGTC GACGC(5/10)
3158 GCGTC
Hin1I 335 GGCGCC G[GA]^CG[CT]C
3157 GGCGTC
HincII 763 GTCGAC GT[CT]^[GA]AC
3136 GTCGAC
HindII 763 GTCGAC GT[CT]^[GA]AC
3136 GTCGAC
KpnI 785 GGTACC GGTAC^C
2124 GGTACC
MflI 3517 GGATCT [GA]^GATC[CT]
4147 AGATCT
NcoI 214 CCATGG C^CATGG
1972 CCATGG
PvuII 1165 CAGCTG CAG^CTG
3862 CAGCTG
SalI 763 GTCGAC G^TCGAC
3136 GTCGAC
SspBI 494 TGTACA T^GTACA
2695 TGTACA
SspI 1743 AATATT AAT^ATT
1763 AATATT
XhoII 3517 GGATCT [GA]^GATC[CT]
4147 AGATCT
XmnI 568 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2944 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbvI BclI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I