##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Aor51HI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BcgI
BglI BglII BlnI BsaAI BsaHI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FauI FseI
FspI HaeII Hin1I KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SapI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 2209 CTTAAG C^TTAAG
AlwNI 569 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2294 GGGCCC GGGCC^C
AseI 3899 ATTAAT AT^TAAT
AsnI 3899 ATTAAT AT^TAAT
BalI 482 TGGCCA TGG^CCA
BamHI 925 GGATCC G^GATCC
BbsI 1552 GAAGAC GAAGAC(2/6)
BbvCI 1002 CCTCAGC CCTCAGC(-5/-2)
BfrI 2209 CTTAAG C^TTAAG
BlpI 3777 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3777 GCTAAGC GC^T[ACGT]AGC
BpuAI 1552 GAAGAC GAAGAC(2/6)
BsaBI 1734 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 852 ACCGGA [AT]^CCGG[AT]
BsmI 3166 GAATGC GAATGC(1/-1)
Bst1107I 4652 GTATAC GTA^TAC
BstAPI 2636 GCACCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4652 GTATAC GTA^TAC
CelII 3777 GCTAAGC GC^T[ACGT]AGC
CfoI 2000 GCGC GCG^C
EcoNI 2609 CCTCTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 751 GATATC GAT^ATC
HhaI 2000 GCGC GCG^C
HinP1I 2000 GCGC G^CGC
HindIII 4257 AAGCTT A^AGCTT
HpaI 654 GTTAAC GTT^AAC
MaeII 2009 ACGT A^CGT
MamI 1734 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 482 TGGCCA TGG^CCA
MscI 482 TGGCCA TGG^CCA
NcoI 204 CCATGG C^CATGG
NdeI 2523 CATATG CA^TATG
PshBI 3899 ATTAAT AT^TAAT
SacI 1843 GAGCTC GAGCT^C
SalI 1072 GTCGAC G^TCGAC
SbfI 3110 CCTGCAGG CCTGCA^GG
ScaI 3665 AGTACT AGT^ACT
SexAI 2455 ACCAGGT A^CC[AT]GGT
Sse8387I 3110 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1889 CCTCAGG CC^T[ACGT]AGG
2496 CCTCAGG
BciVI 3116 GGATAC GTATCC(6/5)
2732 GTATCC
BclI 906 TGATCA T^GATCA
918 TGATCA
BcnI 1877 CCGGG CC^[GC]GG
1933 CCCGG
BfaI 1710 CTAG C^TAG
3809 CTAG
BsaI 849 GAGACC GGTCTC(1/5)
2479 GAGACC
BsmBI 626 CGTCTC CGTCTC(1/5)
2754 CGTCTC
Bsp1407I 610 TGTACA T^GTACA
3750 TGTACA
BsrDI 3050 GCAATG GCAATG(2/0)
3170 GCAATG
BsrGI 610 TGTACA T^GTACA
3750 TGTACA
Bsu36I 1889 CCTCAGG CC^T[ACGT]AGG
2496 CCTCAGG
DraI 4321 TTTAAA TTT^AAA
4526 TTTAAA
DraIII 1373 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2217 CACAAAGTG
DsaI 204 CCATGG C^C[GA][CT]GG
1259 CCACGG
EaeI 482 TGGCCA [CT]^GGCC[GA]
3421 TGGCCG
Eco81I 1889 CCTCAGG CC^T[ACGT]AGG
2496 CCTCAGG
EcoRI 1420 GAATTC G^AATTC
2233 GAATTC
EcoT14I 204 CCATGG C^C[AT][AT]GG
2626 CCTTGG
FbaI 906 TGATCA T^GATCA
918 TGATCA
HgaI 3638 GACGC GACGC(5/10)
457 GCGTC
MaeI 1710 CTAG C^TAG
3809 CTAG
MfeI 2832 CAATTG C^AATTG
3754 CAATTG
MunI 2832 CAATTG C^AATTG
3754 CAATTG
NciI 1877 CCGGG CC^[GC]GG
1933 CCCGG
NspI 3103 GCATGT [GA]CATG^[CT]
4396 ACATGC
PvuII 402 CAGCTG CAG^CTG
2094 CAGCTG
SspBI 610 TGTACA T^GTACA
3750 TGTACA
StuI 1163 AGGCCT AGG^CCT
1331 AGGCCT
StyI 204 CCATGG C^C[AT][AT]GG
2626 CCTTGG
TaqI 1073 TCGA T^CGA
2204 TCGA
TthHB8I 1073 TCGA T^CGA
2204 TCGA
XbaI 1709 TCTAGA T^CTAGA
3808 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaII BanI BanII BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI