################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AgeI ApaI AscI Asp718I AvrII BamHI BbeI BbrPI BcgI BclI BglI BlnI BsaBI BsiWI BspDI BspHI BssHII BssSI BstBI BstEII BstPI ClaI CpoI DrdI EcoNI EcoO65I EcoRV EcoT22I FbaI FseI HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NcoI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 250 GACGTC GACGT^C AccIII 453 TCCGGA T^CCGGA AclI 552 AACGTT AA^CGTT AcyI 250 GACGTC G[GA]^CG[CT]C AhdI 2999 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 375 GATCC GGATC(4/5) AocI 3293 CCTGAGG CC^T[ACGT]AGG Aor51HI 3920 AGCGCT AGC^GCT AspEI 2999 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 48 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 37 TGCGCA TGC^GCA BalI 1125 TGGCCA TGG^CCA BbvCI 1579 GCTGAGG CCTCAGC(-5/-2) BglII 2187 AGATCT A^GATCT BsaAI 1765 CACGTA [CT]AC^GT[GA] BsaHI 250 GACGTC G[GA]^CG[CT]C BseAI 453 TCCGGA T^CCGGA BsiEI 349 CGGCCG CG[GA][CT]^CG BspEI 453 TCCGGA T^CCGGA BsrBI 3937 CCGCTC CCGCTC(-3/-3) BsrFI 317 GCCGGC [GA]^CCGG[CT] BstXI 2018 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 3293 CCTGAGG CC^T[ACGT]AGG Cfr10I 317 GCCGGC [GA]^CCGG[CT] DsaI 3817 CCGTGG C^C[GA][CT]GG EagI 349 CGGCCG C^GGCCG Eam1105I 2999 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 349 CGGCCG C^GGCCG Eco47III 3920 AGCGCT AGC^GCT Eco52I 349 CGGCCG C^GGCCG Eco81I 3293 CCTGAGG CC^T[ACGT]AGG FspI 37 TGCGCA TGC^GCA HaeII 3920 AGCGCT [GA]GCGC^[CT] Hin1I 250 GACGTC G[GA]^CG[CT]C HindIII 1370 AAGCTT A^AGCTT MluNI 1125 TGGCCA TGG^CCA MroI 453 TCCGGA T^CCGGA MscI 1125 TGGCCA TGG^CCA MspA1I 2769 CAGCTG C[AC]G^C[GT]G NaeI 317 GCCGGC GCC^GGC NdeI 4320 CATATG CA^TATG NgoMIV 317 GCCGGC G^CCGGC PflFI 48 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3833 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2919 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 1683 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 552 AACGTT AA^CGTT PvuII 2769 CAGCTG CAG^CTG ScaI 1814 AGTACT AGT^ACT SexAI 2954 ACCTGGT A^CC[AT]GGT TaqI 1062 TCGA T^CGA Tth111I 48 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1062 TCGA T^CGA Van91I 3833 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 285 CCAAGAAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflII 3221 CTTAAG C^TTAAG 3450 CTTAAG AflIII 2424 ACATGT A^C[GA][CT]GT 3384 ACATGT Alw44I 2373 GTGCAC G^TGCAC 2907 GTGCAC ApaLI 2373 GTGCAC G^TGCAC 2907 GTGCAC AseI 3797 ATTAAT AT^TAAT 3811 ATTAAT AsnI 3797 ATTAAT AT^TAAT 3811 ATTAAT BfrI 3221 CTTAAG C^TTAAG 3450 CTTAAG BsaI 1563 GAGACC GGTCTC(1/5) 1918 GAGACC BseRI 466 GAGGAG GAGGAG(10/8) 658 GAGGAG BsmBI 248 GAGACG CGTCTC(1/5) 3734 GAGACG BsmI 1351 GAATGC GAATGC(1/-1) 2348 GAATGC BspLU11I 2424 ACATGT A^CATGT 3384 ACATGT BsrDI 4114 CATTGC GCAATG(2/0) 686 GCAATG BstYI 374 AGATCC [GA]^GATC[CT] 2187 AGATCT DraI 2893 TTTAAA TTT^AAA 3952 TTTAAA EaeI 349 CGGCCG [CT]^GGCC[GA] 1125 TGGCCA EcoRI 1713 GAATTC G^AATTC 4336 GAATTC FauI 3936 CCCGC CCCGC(4/6) 1586 GCGGG HgaI 48 GACGC GACGC(5/10) 3217 GCGTC HincII 2867 GTCAAC GT[CT]^[GA]AC 3403 GTCAAC HindII 2867 GTCAAC GT[CT]^[GA]AC 3403 GTCAAC MflI 374 AGATCC [GA]^GATC[CT] 2187 AGATCT NheI 754 GCTAGC G^CTAGC 2352 GCTAGC PshBI 3797 ATTAAT AT^TAAT 3811 ATTAAT PstI 333 CTGCAG CTGCA^G 427 CTGCAG SmaI 302 CCCGGG CCC^GGG 2613 CCCGGG SspI 1927 AATATT AAT^ATT 2101 AATATT XhoII 374 AGATCC [GA]^GATC[CT] 2187 AGATCT XmaCI 302 CCCGGG C^CCGGG 2613 CCCGGG XmaI 302 CCCGGG C^CCGGG 2613 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI Bst1107I BstAPI BstF5I BstNI BstUI BstZ17I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspBI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI