##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AgeI ApaI AscI Asp718I
AvrII BamHI BbeI BbrPI BcgI
BclI BglI BlnI BsaBI BsiWI
BspDI BspHI BssHII BssSI BstBI
BstEII BstPI ClaI CpoI DrdI
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NcoI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 250 GACGTC GACGT^C
AccIII 453 TCCGGA T^CCGGA
AclI 552 AACGTT AA^CGTT
AcyI 250 GACGTC G[GA]^CG[CT]C
AhdI 2999 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 375 GATCC GGATC(4/5)
AocI 3293 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3920 AGCGCT AGC^GCT
AspEI 2999 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 48 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 37 TGCGCA TGC^GCA
BalI 1125 TGGCCA TGG^CCA
BbvCI 1579 GCTGAGG CCTCAGC(-5/-2)
BglII 2187 AGATCT A^GATCT
BsaAI 1765 CACGTA [CT]AC^GT[GA]
BsaHI 250 GACGTC G[GA]^CG[CT]C
BseAI 453 TCCGGA T^CCGGA
BsiEI 349 CGGCCG CG[GA][CT]^CG
BspEI 453 TCCGGA T^CCGGA
BsrBI 3937 CCGCTC CCGCTC(-3/-3)
BsrFI 317 GCCGGC [GA]^CCGG[CT]
BstXI 2018 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3293 CCTGAGG CC^T[ACGT]AGG
Cfr10I 317 GCCGGC [GA]^CCGG[CT]
DsaI 3817 CCGTGG C^C[GA][CT]GG
EagI 349 CGGCCG C^GGCCG
Eam1105I 2999 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 349 CGGCCG C^GGCCG
Eco47III 3920 AGCGCT AGC^GCT
Eco52I 349 CGGCCG C^GGCCG
Eco81I 3293 CCTGAGG CC^T[ACGT]AGG
FspI 37 TGCGCA TGC^GCA
HaeII 3920 AGCGCT [GA]GCGC^[CT]
Hin1I 250 GACGTC G[GA]^CG[CT]C
HindIII 1370 AAGCTT A^AGCTT
MluNI 1125 TGGCCA TGG^CCA
MroI 453 TCCGGA T^CCGGA
MscI 1125 TGGCCA TGG^CCA
MspA1I 2769 CAGCTG C[AC]G^C[GT]G
NaeI 317 GCCGGC GCC^GGC
NdeI 4320 CATATG CA^TATG
NgoMIV 317 GCCGGC G^CCGGC
PflFI 48 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3833 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2919 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1683 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 552 AACGTT AA^CGTT
PvuII 2769 CAGCTG CAG^CTG
ScaI 1814 AGTACT AGT^ACT
SexAI 2954 ACCTGGT A^CC[AT]GGT
TaqI 1062 TCGA T^CGA
Tth111I 48 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1062 TCGA T^CGA
Van91I 3833 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 285 CCAAGAAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 3221 CTTAAG C^TTAAG
3450 CTTAAG
AflIII 2424 ACATGT A^C[GA][CT]GT
3384 ACATGT
Alw44I 2373 GTGCAC G^TGCAC
2907 GTGCAC
ApaLI 2373 GTGCAC G^TGCAC
2907 GTGCAC
AseI 3797 ATTAAT AT^TAAT
3811 ATTAAT
AsnI 3797 ATTAAT AT^TAAT
3811 ATTAAT
BfrI 3221 CTTAAG C^TTAAG
3450 CTTAAG
BsaI 1563 GAGACC GGTCTC(1/5)
1918 GAGACC
BseRI 466 GAGGAG GAGGAG(10/8)
658 GAGGAG
BsmBI 248 GAGACG CGTCTC(1/5)
3734 GAGACG
BsmI 1351 GAATGC GAATGC(1/-1)
2348 GAATGC
BspLU11I 2424 ACATGT A^CATGT
3384 ACATGT
BsrDI 4114 CATTGC GCAATG(2/0)
686 GCAATG
BstYI 374 AGATCC [GA]^GATC[CT]
2187 AGATCT
DraI 2893 TTTAAA TTT^AAA
3952 TTTAAA
EaeI 349 CGGCCG [CT]^GGCC[GA]
1125 TGGCCA
EcoRI 1713 GAATTC G^AATTC
4336 GAATTC
FauI 3936 CCCGC CCCGC(4/6)
1586 GCGGG
HgaI 48 GACGC GACGC(5/10)
3217 GCGTC
HincII 2867 GTCAAC GT[CT]^[GA]AC
3403 GTCAAC
HindII 2867 GTCAAC GT[CT]^[GA]AC
3403 GTCAAC
MflI 374 AGATCC [GA]^GATC[CT]
2187 AGATCT
NheI 754 GCTAGC G^CTAGC
2352 GCTAGC
PshBI 3797 ATTAAT AT^TAAT
3811 ATTAAT
PstI 333 CTGCAG CTGCA^G
427 CTGCAG
SmaI 302 CCCGGG CCC^GGG
2613 CCCGGG
SspI 1927 AATATT AAT^ATT
2101 AATATT
XhoII 374 AGATCC [GA]^GATC[CT]
2187 AGATCT
XmaCI 302 CCCGGG C^CCGGG
2613 CCCGGG
XmaI 302 CCCGGG C^CCGGG
2613 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI Bst1107I
BstAPI BstF5I BstNI BstUI BstZ17I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspBI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI