##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AhdI ApaI AscI Asp718I AspEI
AspI AviII BamHI BbrPI BciVI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BstAPI BstBI BstEII BstPI
BstXI ClaI CpoI DsaI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HindIII HpaI KpnI
KspI MluI MroI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SmaI SnaBI Sse8387I SwaI
Tth111I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcyI 41 GGCGCC G[GA]^CG[CT]C
AflII 1335 CTTAAG C^TTAAG
Alw44I 3182 GTGCAC G^TGCAC
AocI 1516 CCTCAGG CC^T[ACGT]AGG
Aor51HI 628 AGCGCT AGC^GCT
ApaLI 3182 GTGCAC G^TGCAC
AvrII 652 CCTAGG C^CTAGG
BalI 1565 TGGCCA TGG^CCA
BbeI 41 GGCGCC GGCGC^C
BbvCI 2722 GCTGAGG CCTCAGC(-5/-2)
BcgI 624 GCAGAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 48 CCCGG CC^[GC]GG
BfrI 1335 CTTAAG C^TTAAG
BglII 337 AGATCT A^GATCT
BlnI 652 CCTAGG C^CTAGG
BlpI 1948 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1948 GCTCAGC GC^T[ACGT]AGC
BsaBI 542 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 41 GGCGCC G[GA]^CG[CT]C
BsaI 2350 GAGACC GGTCTC(1/5)
BsmBI 3031 GAGACG CGTCTC(1/5)
BsmI 2747 GCATTC GAATGC(1/-1)
BspMI 1224 GCAGGT ACCTGC(4/8)
BsrBI 52 GAGCGG CCGCTC(-3/-3)
BssSI 425 CTCGTG CACGAG(-5/-1)
Bst1107I 4113 GTATAC GTA^TAC
BstZ17I 4113 GTATAC GTA^TAC
Bsu36I 1516 CCTCAGG CC^T[ACGT]AGG
CelII 1948 GCTCAGC GC^T[ACGT]AGC
DraIII 3245 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 18 CGGCCG C^GGCCG
EclXI 18 CGGCCG C^GGCCG
Eco47III 628 AGCGCT AGC^GCT
Eco52I 18 CGGCCG C^GGCCG
Eco81I 1516 CCTCAGG CC^T[ACGT]AGG
EcoNI 2893 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 41 GGCGCC G[GA]^CG[CT]C
HincII 3148 GTCAAC GT[CT]^[GA]AC
HindII 3148 GTCAAC GT[CT]^[GA]AC
KasI 41 GGCGCC G^GCGCC
MamI 542 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 271 CAATTG C^AATTG
MluNI 1565 TGGCCA TGG^CCA
MscI 1565 TGGCCA TGG^CCA
MunI 271 CAATTG C^AATTG
NaeI 2530 GCCGGC GCC^GGC
NarI 41 GGCGCC GG^CGCC
NciI 48 CCCGG CC^[GC]GG
NgoMIV 2530 GCCGGC G^CCGGC
NspI 1439 GCATGC [GA]CATG^[CT]
PaeR7I 632 CTCGAG C^TCGAG
PshAI 3409 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3210 GAGCTC GAGCT^C
ScaI 2507 AGTACT AGT^ACT
SfoI 41 GGCGCC GGC^GCC
SgrAI 2660 CACCGGTG C[GA]^CCGG[CT]G
SphI 1439 GCATGC GCATG^C
StuI 2729 AGGCCT AGG^CCT
XhoI 632 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3601 GTAGAC GT^[AC][GT]AC
4113 GTATAC
AccII 34 CGCG CG^CG
67 CGCG
AcsI 1322 AAATTC [GA]^AATT[CT]
2143 GAATTT
AgeI 2661 ACCGGT A^CCGGT
2687 ACCGGT
ApoI 1322 AAATTC [GA]^AATT[CT]
2143 GAATTT
AseI 3376 ATTAAT AT^TAAT
3842 ATTAAT
AsnI 3376 ATTAAT AT^TAAT
3842 ATTAAT
BanI 41 GGCGCC G^G[CT][GA]CC
3067 GGCACC
BanII 648 GAGCCC G[GA]GC[CT]^C
3210 GAGCTC
BbsI 662 GAAGAC GAAGAC(2/6)
2998 GAAGAC
BclI 1665 TGATCA T^GATCA
3772 TGATCA
BglI 8 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
30 GCCACGCGGGC
BpuAI 662 GAAGAC GAAGAC(2/6)
2998 GAAGAC
BsaAI 2857 CACGTA [CT]AC^GT[GA]
3371 CACGTA
BsiEI 18 CGGCCG CG[GA][CT]^CG
1655 CGACCG
BsrDI 501 CATTGC GCAATG(2/0)
1932 CATTGC
BstUI 34 CGCG CG^CG
67 CGCG
DraII 701 AGGCCCT [GA]G^G[ACGT]CC[CT]
1308 AGGCCCT
DrdI 1290 GACTGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2213 GACCAAAAAGTC
EarI 522 CTCTTC CTCTTC(1/4)
558 GAAGAG
EcoO109I 701 AGGCCCT [GA]G^G[ACGT]CC[CT]
1308 AGGCCCT
FbaI 1665 TGATCA T^GATCA
3772 TGATCA
HaeII 41 GGCGCC [GA]GCGC^[CT]
628 AGCGCT
Ksp632I 522 CTCTTC CTCTTC(1/4)
558 GAAGAG
MvnI 34 CGCG CG^CG
67 CGCG
PinAI 2661 ACCGGT A^CCGGT
2687 ACCGGT
PshBI 3376 ATTAAT AT^TAAT
3842 ATTAAT
PstI 974 CTGCAG CTGCA^G
1105 CTGCAG
SpeI 1248 ACTAGT A^CTAGT
3138 ACTAGT
SspI 320 AATATT AAT^ATT
1789 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BbvI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI