##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BciVI BfrI BglII
BlnI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BstBI ClaI CpoI
Eam1105I FseI FspI HindIII KasI
KpnI MfeI MluI MroI MunI
NarI NruI NspV PacI PinAI
PshAI PshBI PvuI RsrII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 3168 AACGTT AA^CGTT
AcyI 3131 GGCGTC G[GA]^CG[CT]C
AflIII 1073 ACACGT A^C[GA][CT]GT
Aor51HI 1478 AGCGCT AGC^GCT
ApaI 3085 GGGCCC GGGCC^C
AspI 1135 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1522 CACGTG CAC^GTG
BbvCI 2718 CCTCAGC CCTCAGC(-5/-2)
BcgI 3035 GCACTGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1858 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1858 GCTGAGC GC^T[ACGT]AGC
BsaHI 3131 GGCGTC G[GA]^CG[CT]C
Bsp1407I 3605 TGTACA T^GTACA
BsrBI 387 GAGCGG CCGCTC(-3/-3)
BsrDI 1823 CATTGC GCAATG(2/0)
BsrGI 3605 TGTACA T^GTACA
Bst1107I 2791 GTATAC GTA^TAC
BstAPI 2521 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3376 GGTGACC G^GT[ACGT]ACC
BstPI 3376 GGTGACC G^GT[ACGT]ACC
BstZ17I 2791 GTATAC GTA^TAC
CelII 1858 GCTGAGC GC^T[ACGT]AGC
Eco47III 1478 AGCGCT AGC^GCT
EcoO65I 3376 GGTGACC G^GT[ACGT]ACC
Hin1I 3131 GGCGTC G[GA]^CG[CT]C
HpaI 4462 GTTAAC GTT^AAC
NheI 1725 GCTAGC G^CTAGC
NotI 38 GCGGCCGC GC^GGCCGC
PaeR7I 487 CTCGAG C^TCGAG
PflFI 1135 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1522 CACGTG CAC^GTG
PmeI 3804 GTTTAAAC GTTT^AAAC
PmlI 1522 CACGTG CAC^GTG
PpuMI 4942 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 3168 AACGTT AA^CGTT
SmaI 269 CCCGGG CCC^GGG
SpeI 2154 ACTAGT A^CTAGT
SspBI 3605 TGTACA T^GTACA
SspI 4365 AATATT AAT^ATT
Tth111I 1135 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2309 CCACTCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 487 CTCGAG C^TCGAG
XmaCI 269 CCCGGG C^CCGGG
XmaI 269 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 84 CCTGAGG CC^T[ACGT]AGG
1840 CCTGAGG
BalI 431 TGGCCA TGG^CCA
3263 TGGCCA
BsaAI 1522 CACGTG [CT]AC^GT[GA]
4278 TACGTG
BsaBI 682 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
928 GATGAGGATC
BsaI 1667 GAGACC GGTCTC(1/5)
1676 GAGACC
BsaWI 609 TCCGGT [AT]^CCGG[AT]
3156 TCCGGT
BstYI 933 GGATCT [GA]^GATC[CT]
3653 AGATCC
Bsu36I 84 CCTGAGG CC^T[ACGT]AGG
1840 CCTGAGG
DraI 3805 TTTAAA TTT^AAA
4349 TTTAAA
DraIII 1019 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4224 CACAATGTG
DrdI 2970 GACTAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3379 GACCCCAGAGTC
Eco81I 84 CCTGAGG CC^T[ACGT]AGG
1840 CCTGAGG
EcoRI 2089 GAATTC G^AATTC
2480 GAATTC
EcoRV 1807 GATATC GAT^ATC
3665 GATATC
EcoT22I 4257 ATGCAT ATGCA^T
4371 ATGCAT
HincII 4219 GTCAAC GT[CT]^[GA]AC
4462 GTTAAC
HindII 4219 GTCAAC GT[CT]^[GA]AC
4462 GTTAAC
KspI 42 CCGCGG CCGC^GG
1313 CCGCGG
MamI 682 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
928 GATGAGGATC
MflI 933 GGATCT [GA]^GATC[CT]
3653 AGATCC
MluNI 431 TGGCCA TGG^CCA
3263 TGGCCA
MscI 431 TGGCCA TGG^CCA
3263 TGGCCA
NaeI 103 GCCGGC GCC^GGC
144 GCCGGC
NcoI 188 CCATGG C^CATGG
726 CCATGG
NgoMIV 103 GCCGGC G^CCGGC
144 GCCGGC
NsiI 4257 ATGCAT ATGCA^T
4371 ATGCAT
SacI 460 GAGCTC GAGCT^C
2363 GAGCTC
SacII 42 CCGCGG CCGC^GG
1313 CCGCGG
StuI 437 AGGCCT AGG^CCT
3232 AGGCCT
XhoII 933 GGATCT [GA]^GATC[CT]
3653 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssHII BssKI BssSI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XmnI