##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I Aor51HI ApaLI AscI AspI
AvaI AviII AvrII BbeI BbrPI
BbsI BcgI BciVI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BssHII BstBI BstEII BstPI
CelII ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3078 GGTACC G^GTACC
AccI 652 GTATAC GT^[AC][GT]AC
AccII 3620 CGCG CG^CG
AflII 3772 CTTAAG C^TTAAG
AflIII 3993 ACGTGT A^C[GA][CT]GT
AhdI 2706 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1582 CCTCAGG CC^T[ACGT]AGG
ApaI 2997 GGGCCC GGGCC^C
Asp700I 632 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3078 GGTACC G^GTACC
AspEI 2706 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcnI 2267 CCCGG CC^[GC]GG
BfrI 3772 CTTAAG C^TTAAG
BglI 2990 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspHI 425 TCATGA T^CATGA
BsrFI 221 ACCGGC [GA]^CCGG[CT]
BssSI 2522 CACGAG CACGAG(-5/-1)
Bst1107I 652 GTATAC GTA^TAC
BstAPI 3286 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3620 CGCG CG^CG
BstXI 2927 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 652 GTATAC GTA^TAC
Bsu36I 1582 CCTCAGG CC^T[ACGT]AGG
Cfr10I 221 ACCGGC [GA]^CCGG[CT]
Eam1105I 2706 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1582 CCTCAGG CC^T[ACGT]AGG
EcoNI 3159 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 2190 GTCAAC GT[CT]^[GA]AC
HindII 2190 GTCAAC GT[CT]^[GA]AC
HindIII 1482 AAGCTT A^AGCTT
KpnI 3078 GGTACC GGTAC^C
MvnI 3620 CGCG CG^CG
NciI 2267 CCCGG CC^[GC]GG
PflMI 1342 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 425 TCATGA T^CATGA
ScaI 3836 AGTACT AGT^ACT
SexAI 228 ACCAGGT A^CC[AT]GGT
StuI 937 AGGCCT AGG^CCT
SwaI 1109 ATTTAAAT ATTT^AAAT
Van91I 1342 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 135 TCTAGA T^CTAGA
XmnI 632 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 1590 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
2444 CAGCTCCTG
AseI 795 ATTAAT AT^TAAT
1081 ATTAAT
AsnI 795 ATTAAT AT^TAAT
1081 ATTAAT
BalI 894 TGGCCA TGG^CCA
2784 TGGCCA
BclI 147 TGATCA T^GATCA
1125 TGATCA
BsrDI 536 GCAATG GCAATG(2/0)
1537 GCAATG
CfoI 3103 GCGC GCG^C
3734 GCGC
DraI 1110 TTTAAA TTT^AAA
3583 TTTAAA
EcoRI 672 GAATTC G^AATTC
1248 GAATTC
FbaI 147 TGATCA T^GATCA
1125 TGATCA
HaeII 3102 AGCGCC [GA]GCGC^[CT]
3733 GGCGCT
HapII 222 CCGG C^CGG
2268 CCGG
HhaI 3103 GCGC GCG^C
3734 GCGC
HinP1I 3103 GCGC G^CGC
3734 GCGC
HpaII 222 CCGG C^CGG
2268 CCGG
MluNI 894 TGGCCA TGG^CCA
2784 TGGCCA
MscI 894 TGGCCA TGG^CCA
2784 TGGCCA
MspI 222 CCGG C^CGG
2268 CCGG
NcoI 891 CCATGG C^CATGG
2993 CCATGG
NspI 294 GCATGT [GA]CATG^[CT]
761 ACATGC
PshBI 795 ATTAAT AT^TAAT
1081 ATTAAT
PstI 5 CTGCAG CTGCA^G
1918 CTGCAG
SacI 1814 GAGCTC GAGCT^C
3088 GAGCTC
SapI 2066 GCTCTTC GCTCTTC(1/4)
3040 GCTCTTC
SfcI 5 CTGCAG C^T[GA][CT]AG
1918 CTGCAG
SspI 1084 AATATT AAT^ATT
1576 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BamHI BanI
BanII BbvCI BbvI BfaI BglII
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII