################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## Acc65I AccII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII BamHI BcgI BglI BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI BsmBI BspHI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HgaI HincII HindII HpaI KpnI KspI MamI MluI MvnI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccIII 326 TCCGGA T^CCGGA AclI 3282 AACGTT AA^CGTT AflII 2062 CTTAAG C^TTAAG AocI 389 CCTGAGG CC^T[ACGT]AGG AspHI 2139 GTGCTC G[AT]GC[AT]^C AspI 3119 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 900 CCCGAG C^[CT]CG[GA]G AvrII 2234 CCTAGG C^CTAGG BalI 1891 TGGCCA TGG^CCA BanII 1965 GAGCCC G[GA]GC[CT]^C BbeI 3233 GGCGCC GGCGC^C BbrPI 3337 CACGTG CAC^GTG BbvCI 1969 CCTCAGC CCTCAGC(-5/-2) BciVI 199 GTATCC GTATCC(6/5) BclI 2250 TGATCA T^GATCA BcnI 2104 CCGGG CC^[GC]GG BfrI 2062 CTTAAG C^TTAAG BlnI 2234 CCTAGG C^CTAGG BsaAI 3337 CACGTG [CT]AC^GT[GA] BsaWI 326 TCCGGA [AT]^CCGG[AT] BseAI 326 TCCGGA T^CCGGA BsiHKAI 2139 GTGCTC G[AT]GC[AT]^C BsoBI 900 CCCGAG C^[CT]CG[GA]G Bsp1407I 593 TGTACA T^GTACA BspDI 1536 ATCGAT AT^CGAT BspEI 326 TCCGGA T^CCGGA BspMI 2724 ACCTGC ACCTGC(4/8) BsrBI 3320 GAGCGG CCGCTC(-3/-3) BsrGI 593 TGTACA T^GTACA BstAPI 581 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1760 CCAGAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 389 CCTGAGG CC^T[ACGT]AGG ClaI 1536 ATCGAT AT^CGAT Eco81I 389 CCTGAGG CC^T[ACGT]AGG FauI 146 GCGGG CCCGC(4/6) FbaI 2250 TGATCA T^GATCA HaeII 3233 GGCGCC [GA]GCGC^[CT] KasI 3233 GGCGCC G^GCGCC MluNI 1891 TGGCCA TGG^CCA MroI 326 TCCGGA T^CCGGA MscI 1891 TGGCCA TGG^CCA MslI 1981 CACTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 3233 GGCGCC GG^CGCC NciI 2104 CCGGG CC^[GC]GG NheI 142 GCTAGC G^CTAGC PflFI 3119 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 739 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3337 CACGTG CAC^GTG PmlI 3337 CACGTG CAC^GTG PpuMI 2328 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1954 GACGGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3282 AACGTT AA^CGTT SbfI 2725 CCTGCAGG CCTGCA^GG SfoI 3233 GGCGCC GGC^GCC Sse8387I 2725 CCTGCAGG CCTGCA^GG SspBI 593 TGTACA T^GTACA SspI 1626 AATATT AAT^ATT StuI 2878 AGGCCT AGG^CCT Tth111I 3119 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 739 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 1742 GACGTC GACGT^C 2521 GACGTC AvaII 623 GGTCC G^G[AT]CC 2329 GGACC BanI 947 GGTGCC G^G[CT][GA]CC 3233 GGCGCC BbsI 1728 GAAGAC GAAGAC(2/6) 3095 GAAGAC BglII 114 AGATCT A^GATCT 1390 AGATCT BpuAI 1728 GAAGAC GAAGAC(2/6) 3095 GAAGAC BsmI 1591 GAATGC GAATGC(1/-1) 3581 GAATGC CfoI 1116 GCGC GCG^C 3234 GCGC DraI 991 TTTAAA TTT^AAA 3751 TTTAAA DraII 1335 AGGGCCT [GA]G^G[ACGT]CC[CT] 2328 AGGACCT EaeI 465 TGGCCG [CT]^GGCC[GA] 1891 TGGCCA EcoNI 1874 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2339 CCTTACTCAGG EcoO109I 1335 AGGGCCT [GA]G^G[ACGT]CC[CT] 2328 AGGACCT EcoRI 693 GAATTC G^AATTC 1563 GAATTC HhaI 1116 GCGC GCG^C 3234 GCGC HinP1I 1116 GCGC G^CGC 3234 GCGC HindIII 679 AAGCTT A^AGCTT 3510 AAGCTT NcoI 971 CCATGG C^CATGG 2421 CCATGG SmlI 2062 CTTAAG C^T[CT][GA]AG 2079 CTCAAG SpeI 1519 ACTAGT A^CTAGT 2227 ACTAGT SphI 2616 GCATGC GCATG^C 3797 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I BbvI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI