##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BamHI
BcgI BglI BlpI Bpu1102I BsaBI
BsaI BsiEI BsiWI BsmBI BspHI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HgaI HincII
HindII HpaI KpnI KspI MamI
MluI MvnI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SwaI XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 326 TCCGGA T^CCGGA
AclI 3282 AACGTT AA^CGTT
AflII 2062 CTTAAG C^TTAAG
AocI 389 CCTGAGG CC^T[ACGT]AGG
AspHI 2139 GTGCTC G[AT]GC[AT]^C
AspI 3119 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 900 CCCGAG C^[CT]CG[GA]G
AvrII 2234 CCTAGG C^CTAGG
BalI 1891 TGGCCA TGG^CCA
BanII 1965 GAGCCC G[GA]GC[CT]^C
BbeI 3233 GGCGCC GGCGC^C
BbrPI 3337 CACGTG CAC^GTG
BbvCI 1969 CCTCAGC CCTCAGC(-5/-2)
BciVI 199 GTATCC GTATCC(6/5)
BclI 2250 TGATCA T^GATCA
BcnI 2104 CCGGG CC^[GC]GG
BfrI 2062 CTTAAG C^TTAAG
BlnI 2234 CCTAGG C^CTAGG
BsaAI 3337 CACGTG [CT]AC^GT[GA]
BsaWI 326 TCCGGA [AT]^CCGG[AT]
BseAI 326 TCCGGA T^CCGGA
BsiHKAI 2139 GTGCTC G[AT]GC[AT]^C
BsoBI 900 CCCGAG C^[CT]CG[GA]G
Bsp1407I 593 TGTACA T^GTACA
BspDI 1536 ATCGAT AT^CGAT
BspEI 326 TCCGGA T^CCGGA
BspMI 2724 ACCTGC ACCTGC(4/8)
BsrBI 3320 GAGCGG CCGCTC(-3/-3)
BsrGI 593 TGTACA T^GTACA
BstAPI 581 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1760 CCAGAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 389 CCTGAGG CC^T[ACGT]AGG
ClaI 1536 ATCGAT AT^CGAT
Eco81I 389 CCTGAGG CC^T[ACGT]AGG
FauI 146 GCGGG CCCGC(4/6)
FbaI 2250 TGATCA T^GATCA
HaeII 3233 GGCGCC [GA]GCGC^[CT]
KasI 3233 GGCGCC G^GCGCC
MluNI 1891 TGGCCA TGG^CCA
MroI 326 TCCGGA T^CCGGA
MscI 1891 TGGCCA TGG^CCA
MslI 1981 CACTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 3233 GGCGCC GG^CGCC
NciI 2104 CCGGG CC^[GC]GG
NheI 142 GCTAGC G^CTAGC
PflFI 3119 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 739 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3337 CACGTG CAC^GTG
PmlI 3337 CACGTG CAC^GTG
PpuMI 2328 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1954 GACGGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3282 AACGTT AA^CGTT
SbfI 2725 CCTGCAGG CCTGCA^GG
SfoI 3233 GGCGCC GGC^GCC
Sse8387I 2725 CCTGCAGG CCTGCA^GG
SspBI 593 TGTACA T^GTACA
SspI 1626 AATATT AAT^ATT
StuI 2878 AGGCCT AGG^CCT
Tth111I 3119 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 739 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 1742 GACGTC GACGT^C
2521 GACGTC
AvaII 623 GGTCC G^G[AT]CC
2329 GGACC
BanI 947 GGTGCC G^G[CT][GA]CC
3233 GGCGCC
BbsI 1728 GAAGAC GAAGAC(2/6)
3095 GAAGAC
BglII 114 AGATCT A^GATCT
1390 AGATCT
BpuAI 1728 GAAGAC GAAGAC(2/6)
3095 GAAGAC
BsmI 1591 GAATGC GAATGC(1/-1)
3581 GAATGC
CfoI 1116 GCGC GCG^C
3234 GCGC
DraI 991 TTTAAA TTT^AAA
3751 TTTAAA
DraII 1335 AGGGCCT [GA]G^G[ACGT]CC[CT]
2328 AGGACCT
EaeI 465 TGGCCG [CT]^GGCC[GA]
1891 TGGCCA
EcoNI 1874 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2339 CCTTACTCAGG
EcoO109I 1335 AGGGCCT [GA]G^G[ACGT]CC[CT]
2328 AGGACCT
EcoRI 693 GAATTC G^AATTC
1563 GAATTC
HhaI 1116 GCGC GCG^C
3234 GCGC
HinP1I 1116 GCGC G^CGC
3234 GCGC
HindIII 679 AAGCTT A^AGCTT
3510 AAGCTT
NcoI 971 CCATGG C^CATGG
2421 CCATGG
SmlI 2062 CTTAAG C^T[CT][GA]AG
2079 CTCAAG
SpeI 1519 ACTAGT A^CTAGT
2227 ACTAGT
SphI 2616 GCATGC GCATG^C
3797 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I BbvI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MspA1I
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI