##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbeI BcgI BglI
BlnI BsaBI BsaHI BsiEI BsiWI
Bsp1407I BspDI BsrGI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI Hin1I HpaI KasI
KpnI MamI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SfoI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2808 GTAGAC GT^[AC][GT]AC
AflII 3551 CTTAAG C^TTAAG
BbrPI 1120 CACGTG CAC^GTG
BfrI 3551 CTTAAG C^TTAAG
BglII 4159 AGATCT A^GATCT
BlpI 1995 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1995 GCTGAGC GC^T[ACGT]AGC
BsaI 1980 GGTCTC GGTCTC(1/5)
BsmBI 2026 CGTCTC CGTCTC(1/5)
BspHI 104 TCATGA T^CATGA
BsrBI 2353 GAGCGG CCGCTC(-3/-3)
BstAPI 1052 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1257 CCAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1995 GCTGAGC GC^T[ACGT]AGC
DraIII 1339 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 4166 ATGCAT ATGCA^T
HgaI 457 GCGTC GACGC(5/10)
KspI 40 CCGCGG CCGC^GG
NdeI 3806 CATATG CA^TATG
NsiI 4166 ATGCAT ATGCA^T
PmaCI 1120 CACGTG CAC^GTG
PmlI 1120 CACGTG CAC^GTG
PshAI 4303 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 104 TCATGA T^CATGA
SacII 40 CCGCGG CCGC^GG
SapI 2451 GAAGAGC GCTCTTC(1/4)
SgrAI 17 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 94 CCCGGG CCC^GGG
XmaCI 94 CCCGGG C^CCGGG
XmaI 94 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccII 41 CGCG CG^CG
456 CGCG
AccIII 500 TCCGGA T^CCGGA
2333 TCCGGA
AflIII 853 ACATGT A^C[GA][CT]GT
3146 ACATGT
Alw44I 6 GTGCAC G^TGCAC
796 GTGCAC
AocI 967 CCTGAGG CC^T[ACGT]AGG
1435 CCTGAGG
ApaLI 6 GTGCAC G^TGCAC
796 GTGCAC
AseI 3970 ATTAAT AT^TAAT
4542 ATTAAT
AsnI 3970 ATTAAT AT^TAAT
4542 ATTAAT
Asp700I 233 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3634 GAAAGAATTC
AspI 168 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
715 GACGTTGTC
AvaI 94 CCCGGG C^[CT]CG[GA]G
1228 CCCGAG
BclI 263 TGATCA T^GATCA
4147 TGATCA
BsaAI 316 TACGTG [CT]AC^GT[GA]
1120 CACGTG
BsaWI 500 TCCGGA [AT]^CCGG[AT]
2333 TCCGGA
BseAI 500 TCCGGA T^CCGGA
2333 TCCGGA
BsoBI 94 CCCGGG C^[CT]CG[GA]G
1228 CCCGAG
BspEI 500 TCCGGA T^CCGGA
2333 TCCGGA
BspLU11I 853 ACATGT A^CATGT
3146 ACATGT
BstEII 1632 GGTGACC G^GT[ACGT]ACC
2061 GGTGACC
BstPI 1632 GGTGACC G^GT[ACGT]ACC
2061 GGTGACC
BstUI 41 CGCG CG^CG
456 CGCG
Bsu36I 967 CCTGAGG CC^T[ACGT]AGG
1435 CCTGAGG
CfoI 1042 GCGC GCG^C
1423 GCGC
Eco81I 967 CCTGAGG CC^T[ACGT]AGG
1435 CCTGAGG
EcoO65I 1632 GGTGACC G^GT[ACGT]ACC
2061 GGTGACC
EcoRV 496 GATATC GAT^ATC
2578 GATATC
FbaI 263 TGATCA T^GATCA
4147 TGATCA
HaeII 1041 GGCGCT [GA]GCGC^[CT]
1422 GGCGCT
HhaI 1042 GCGC GCG^C
1423 GCGC
HinP1I 1042 GCGC G^CGC
1423 GCGC
MroI 500 TCCGGA T^CCGGA
2333 TCCGGA
MvnI 41 CGCG CG^CG
456 CGCG
NcoI 617 CCATGG C^CATGG
2073 CCATGG
PflFI 168 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
715 GACGTTGTC
PflMI 2068 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCAGTACTTGG
PpuMI 1598 AGGACCT [GA]G^G[AT]CC[CT]
3246 AGGTCCC
PshBI 3970 ATTAAT AT^TAAT
4542 ATTAAT
PstI 1573 CTGCAG CTGCA^G
3195 CTGCAG
ScaI 1463 AGTACT AGT^ACT
3416 AGTACT
Tth111I 168 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
715 GACGTTGTC
Van91I 2068 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCAGTACTTGG
XbaI 217 TCTAGA T^CTAGA
3010 TCTAGA
XmnI 233 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3634 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstBI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
NspV PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI SpeI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII