################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI Aor51HI AscI Asp718I AspEI AviII AvrII BbeI BcgI BglI BlnI BsaBI BsaHI BsiEI BsiWI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI Hin1I HpaI KasI KpnI MamI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfoI SnaBI SphI Sse8387I SspBI SspI StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 2808 GTAGAC GT^[AC][GT]AC AflII 3551 CTTAAG C^TTAAG BbrPI 1120 CACGTG CAC^GTG BfrI 3551 CTTAAG C^TTAAG BglII 4159 AGATCT A^GATCT BlpI 1995 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1995 GCTGAGC GC^T[ACGT]AGC BsaI 1980 GGTCTC GGTCTC(1/5) BsmBI 2026 CGTCTC CGTCTC(1/5) BspHI 104 TCATGA T^CATGA BsrBI 2353 GAGCGG CCGCTC(-3/-3) BstAPI 1052 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1257 CCAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1995 GCTGAGC GC^T[ACGT]AGC DraIII 1339 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 4166 ATGCAT ATGCA^T HgaI 457 GCGTC GACGC(5/10) KspI 40 CCGCGG CCGC^GG NdeI 3806 CATATG CA^TATG NsiI 4166 ATGCAT ATGCA^T PmaCI 1120 CACGTG CAC^GTG PmlI 1120 CACGTG CAC^GTG PshAI 4303 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 104 TCATGA T^CATGA SacII 40 CCGCGG CCGC^GG SapI 2451 GAAGAGC GCTCTTC(1/4) SgrAI 17 CGCCGGTG C[GA]^CCGG[CT]G SmaI 94 CCCGGG CCC^GGG XmaCI 94 CCCGGG C^CCGGG XmaI 94 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccII 41 CGCG CG^CG 456 CGCG AccIII 500 TCCGGA T^CCGGA 2333 TCCGGA AflIII 853 ACATGT A^C[GA][CT]GT 3146 ACATGT Alw44I 6 GTGCAC G^TGCAC 796 GTGCAC AocI 967 CCTGAGG CC^T[ACGT]AGG 1435 CCTGAGG ApaLI 6 GTGCAC G^TGCAC 796 GTGCAC AseI 3970 ATTAAT AT^TAAT 4542 ATTAAT AsnI 3970 ATTAAT AT^TAAT 4542 ATTAAT Asp700I 233 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3634 GAAAGAATTC AspI 168 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 715 GACGTTGTC AvaI 94 CCCGGG C^[CT]CG[GA]G 1228 CCCGAG BclI 263 TGATCA T^GATCA 4147 TGATCA BsaAI 316 TACGTG [CT]AC^GT[GA] 1120 CACGTG BsaWI 500 TCCGGA [AT]^CCGG[AT] 2333 TCCGGA BseAI 500 TCCGGA T^CCGGA 2333 TCCGGA BsoBI 94 CCCGGG C^[CT]CG[GA]G 1228 CCCGAG BspEI 500 TCCGGA T^CCGGA 2333 TCCGGA BspLU11I 853 ACATGT A^CATGT 3146 ACATGT BstEII 1632 GGTGACC G^GT[ACGT]ACC 2061 GGTGACC BstPI 1632 GGTGACC G^GT[ACGT]ACC 2061 GGTGACC BstUI 41 CGCG CG^CG 456 CGCG Bsu36I 967 CCTGAGG CC^T[ACGT]AGG 1435 CCTGAGG CfoI 1042 GCGC GCG^C 1423 GCGC Eco81I 967 CCTGAGG CC^T[ACGT]AGG 1435 CCTGAGG EcoO65I 1632 GGTGACC G^GT[ACGT]ACC 2061 GGTGACC EcoRV 496 GATATC GAT^ATC 2578 GATATC FbaI 263 TGATCA T^GATCA 4147 TGATCA HaeII 1041 GGCGCT [GA]GCGC^[CT] 1422 GGCGCT HhaI 1042 GCGC GCG^C 1423 GCGC HinP1I 1042 GCGC G^CGC 1423 GCGC MroI 500 TCCGGA T^CCGGA 2333 TCCGGA MvnI 41 CGCG CG^CG 456 CGCG NcoI 617 CCATGG C^CATGG 2073 CCATGG PflFI 168 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 715 GACGTTGTC PflMI 2068 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3414 CCAGTACTTGG PpuMI 1598 AGGACCT [GA]G^G[AT]CC[CT] 3246 AGGTCCC PshBI 3970 ATTAAT AT^TAAT 4542 ATTAAT PstI 1573 CTGCAG CTGCA^G 3195 CTGCAG ScaI 1463 AGTACT AGT^ACT 3416 AGTACT Tth111I 168 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 715 GACGTTGTC Van91I 2068 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3414 CCAGTACTTGG XbaI 217 TCTAGA T^CTAGA 3010 TCTAGA XmnI 233 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3634 GAAAGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstBI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI NspV PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI SpeI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII