##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AspI AviII AvrII BamHI
BbeI BbrPI BlnI BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BspDI BspEI BspHI BsrDI BssHII
BssSI BstBI BstEII BstPI BstUI
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I FauI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 3584 CTTAAG C^TTAAG
AhdI 2303 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 108 GATCC GGATC(4/5)
ApaI 2919 GGGCCC GGGCC^C
AspEI 2303 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2649 CTCGGG C^[CT]CG[GA]G
BalI 166 TGGCCA TGG^CCA
BanI 4023 GGTGCC G^G[CT][GA]CC
BcgI 1685 CGAAATCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3223 GTATCC GTATCC(6/5)
BclI 2708 TGATCA T^GATCA
BcnI 564 CCCGG CC^[GC]GG
BfrI 3584 CTTAAG C^TTAAG
BlpI 2317 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2317 GCTCAGC GC^T[ACGT]AGC
BsgI 1742 GTGCAG GTGCAG(16/14)
BsoBI 2649 CTCGGG C^[CT]CG[GA]G
Bsp1407I 916 TGTACA T^GTACA
BsrGI 916 TGTACA T^GTACA
CelII 2317 GCTCAGC GC^T[ACGT]AGC
DraI 3686 TTTAAA TTT^AAA
DraIII 996 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 166 TGGCCA [CT]^GGCC[GA]
Eam1105I 2303 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1655 ATGCAT ATGCA^T
FbaI 2708 TGATCA T^GATCA
HindIII 1433 AAGCTT A^AGCTT
MfeI 723 CAATTG C^AATTG
MluNI 166 TGGCCA TGG^CCA
MscI 166 TGGCCA TGG^CCA
MunI 723 CAATTG C^AATTG
NciI 564 CCCGG CC^[GC]GG
NdeI 4468 CATATG CA^TATG
NsiI 1655 ATGCAT ATGCA^T
PshAI 2181 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2508 GAAGAGC GCTCTTC(1/4)
SexAI 3450 ACCAGGT A^CC[AT]GGT
SspBI 916 TGTACA T^GTACA
SspI 3917 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 1358 GACGCC G[GA]^CG[CT]C
2496 GGCGTC
AocI 338 CCTAAGG CC^T[ACGT]AGG
1982 CCTGAGG
AseI 1573 ATTAAT AT^TAAT
2981 ATTAAT
AsnI 1573 ATTAAT AT^TAAT
2981 ATTAAT
BbvCI 4285 CCTCAGC CCTCAGC(-5/-2)
359 GCTGAGG
BglI 2159 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2241 GCCTGGAGGGC
BglII 172 AGATCT A^GATCT
216 AGATCT
BsaHI 1358 GACGCC G[GA]^CG[CT]C
2496 GGCGTC
BsaI 3142 GAGACC GGTCTC(1/5)
858 GGTCTC
BsmI 936 GCATTC GAATGC(1/-1)
1629 GCATTC
BspMI 3519 ACCTGC ACCTGC(4/8)
2376 GCAGGT
BsrBI 970 CCGCTC CCGCTC(-3/-3)
4 GAGCGG
BsrFI 855 GCCGGT [GA]^CCGG[CT]
1155 GCCGGT
BstAPI 1173 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4017 GCAGTAGGTGC
BstYI 172 AGATCT [GA]^GATC[CT]
216 AGATCT
Bsu36I 338 CCTAAGG CC^T[ACGT]AGG
1982 CCTGAGG
CfoI 21 GCGC GCG^C
1549 GCGC
Cfr10I 855 GCCGGT [GA]^CCGG[CT]
1155 GCCGGT
DsaI 2176 CCATGG C^C[GA][CT]GG
2659 CCATGG
Eco81I 338 CCTAAGG CC^T[ACGT]AGG
1982 CCTGAGG
EcoNI 1013 CCTAAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1285 CCTAGATGAGG
EcoRV 668 GATATC GAT^ATC
2022 GATATC
HhaI 21 GCGC GCG^C
1549 GCGC
Hin1I 1358 GACGCC G[GA]^CG[CT]C
2496 GGCGTC
HinP1I 21 GCGC G^CGC
1549 GCGC
MflI 172 AGATCT [GA]^GATC[CT]
216 AGATCT
MspA1I 1069 CAGCTG C[AC]G^C[GT]G
4012 CAGCTG
NcoI 2176 CCATGG C^CATGG
2659 CCATGG
PflMI 1346 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2397 CCAATACATGG
PshBI 1573 ATTAAT AT^TAAT
2981 ATTAAT
PvuII 1069 CAGCTG CAG^CTG
4012 CAGCTG
TaqI 1518 TCGA T^CGA
2074 TCGA
TthHB8I 1518 TCGA T^CGA
2074 TCGA
Van91I 1346 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2397 CCAATACATGG
XbaI 3095 TCTAGA T^CTAGA
3780 TCTAGA
XcmI 950 CCAGAAGGTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1148 CCACCTAGCCGGTGG
XhoII 172 AGATCT [GA]^GATC[CT]
216 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwNI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspLU11I BsrI BssKI Bst1107I
BstF5I BstNI BstXI BstZ17I Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI