##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
BbrPI BciVI BclI BsaAI BsaBI
BseAI BsiWI BspDI BspEI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI DraIII Eam1105I Eco47III
EcoO65I EcoRI EcoT22I FbaI HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3571 GTAGAC GT^[AC][GT]AC
AflII 3476 CTTAAG C^TTAAG
AgeI 2013 ACCGGT A^CCGGT
Alw44I 514 GTGCAC G^TGCAC
ApaLI 514 GTGCAC G^TGCAC
Asp700I 4142 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1037 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 202 CCTAGG C^CTAGG
BamHI 3455 GGATCC G^GATCC
BfrI 3476 CTTAAG C^TTAAG
BlnI 202 CCTAGG C^CTAGG
BsaWI 2013 ACCGGT [AT]^CCGG[AT]
BsiEI 1887 CGGCCG CG[GA][CT]^CG
BsmBI 1958 GAGACG CGTCTC(1/5)
BssHII 34 GCGCGC G^CGCGC
CpoI 2067 CGGACCG CG^G[AT]CCG
EagI 1887 CGGCCG C^GGCCG
EclXI 1887 CGGCCG C^GGCCG
Eco52I 1887 CGGCCG C^GGCCG
EcoNI 3005 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 111 GGCCGGCC GGCCGG^CC
KspI 436 CCGCGG CCGC^GG
NheI 214 GCTAGC G^CTAGC
PflFI 1037 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2013 ACCGGT A^CCGGT
RsrII 2067 CGGACCG CG^G[AT]CCG
SacII 436 CCGCGG CCGC^GG
SapI 571 GCTCTTC GCTCTTC(1/4)
SbfI 1909 CCTGCAGG CCTGCA^GG
SphI 3951 GCATGC GCATG^C
Sse8387I 1909 CCTGCAGG CCTGCA^GG
StuI 2297 AGGCCT AGG^CCT
Tth111I 1037 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4142 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 1556 GACGTC GACGT^C
1811 GACGTC
AocI 3827 CCTGAGG CC^T[ACGT]AGG
3919 CCTTAGG
AviII 705 TGCGCA TGC^GCA
4055 TGCGCA
BbsI 2709 GAAGAC GAAGAC(2/6)
4284 GTCTTC
BglII 915 AGATCT A^GATCT
3290 AGATCT
BlpI 2046 GCTCAGC GC^T[ACGT]AGC
3503 GCTAAGC
Bpu1102I 2046 GCTCAGC GC^T[ACGT]AGC
3503 GCTAAGC
BpuAI 2709 GAAGAC GAAGAC(2/6)
4284 GTCTTC
Bsp1407I 1605 TGTACA T^GTACA
1869 TGTACA
BspLU11I 1602 ACATGT A^CATGT
3600 ACATGT
BsrDI 2674 CATTGC GCAATG(2/0)
2809 GCAATG
BsrGI 1605 TGTACA T^GTACA
1869 TGTACA
Bsu36I 3827 CCTGAGG CC^T[ACGT]AGG
3919 CCTTAGG
CelII 2046 GCTCAGC GC^T[ACGT]AGC
3503 GCTAAGC
Eco81I 3827 CCTGAGG CC^T[ACGT]AGG
3919 CCTTAGG
FspI 705 TGCGCA TGC^GCA
4055 TGCGCA
HindIII 3360 AAGCTT A^AGCTT
3506 AAGCTT
NaeI 112 GCCGGC GCC^GGC
1885 GCCGGC
NcoI 1986 CCATGG C^CATGG
3582 CCATGG
NgoMIV 112 GCCGGC G^CCGGC
1885 GCCGGC
PpuMI 2543 GGGACCT [GA]G^G[AT]CC[CT]
3480 AGGACCT
PvuII 2122 CAGCTG CAG^CTG
2830 CAGCTG
SspBI 1605 TGTACA T^GTACA
1869 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmaCI
XmaI