################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AccIII AclI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI BbrPI BciVI BclI BsaAI BsaBI BseAI BsiWI BspDI BspEI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoT22I FbaI HpaI KpnI MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SwaI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 3571 GTAGAC GT^[AC][GT]AC AflII 3476 CTTAAG C^TTAAG AgeI 2013 ACCGGT A^CCGGT Alw44I 514 GTGCAC G^TGCAC ApaLI 514 GTGCAC G^TGCAC Asp700I 4142 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1037 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 202 CCTAGG C^CTAGG BamHI 3455 GGATCC G^GATCC BfrI 3476 CTTAAG C^TTAAG BlnI 202 CCTAGG C^CTAGG BsaWI 2013 ACCGGT [AT]^CCGG[AT] BsiEI 1887 CGGCCG CG[GA][CT]^CG BsmBI 1958 GAGACG CGTCTC(1/5) BssHII 34 GCGCGC G^CGCGC CpoI 2067 CGGACCG CG^G[AT]CCG EagI 1887 CGGCCG C^GGCCG EclXI 1887 CGGCCG C^GGCCG Eco52I 1887 CGGCCG C^GGCCG EcoNI 3005 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 111 GGCCGGCC GGCCGG^CC KspI 436 CCGCGG CCGC^GG NheI 214 GCTAGC G^CTAGC PflFI 1037 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2013 ACCGGT A^CCGGT RsrII 2067 CGGACCG CG^G[AT]CCG SacII 436 CCGCGG CCGC^GG SapI 571 GCTCTTC GCTCTTC(1/4) SbfI 1909 CCTGCAGG CCTGCA^GG SphI 3951 GCATGC GCATG^C Sse8387I 1909 CCTGCAGG CCTGCA^GG StuI 2297 AGGCCT AGG^CCT Tth111I 1037 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 4142 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 1556 GACGTC GACGT^C 1811 GACGTC AocI 3827 CCTGAGG CC^T[ACGT]AGG 3919 CCTTAGG AviII 705 TGCGCA TGC^GCA 4055 TGCGCA BbsI 2709 GAAGAC GAAGAC(2/6) 4284 GTCTTC BglII 915 AGATCT A^GATCT 3290 AGATCT BlpI 2046 GCTCAGC GC^T[ACGT]AGC 3503 GCTAAGC Bpu1102I 2046 GCTCAGC GC^T[ACGT]AGC 3503 GCTAAGC BpuAI 2709 GAAGAC GAAGAC(2/6) 4284 GTCTTC Bsp1407I 1605 TGTACA T^GTACA 1869 TGTACA BspLU11I 1602 ACATGT A^CATGT 3600 ACATGT BsrDI 2674 CATTGC GCAATG(2/0) 2809 GCAATG BsrGI 1605 TGTACA T^GTACA 1869 TGTACA Bsu36I 3827 CCTGAGG CC^T[ACGT]AGG 3919 CCTTAGG CelII 2046 GCTCAGC GC^T[ACGT]AGC 3503 GCTAAGC Eco81I 3827 CCTGAGG CC^T[ACGT]AGG 3919 CCTTAGG FspI 705 TGCGCA TGC^GCA 4055 TGCGCA HindIII 3360 AAGCTT A^AGCTT 3506 AAGCTT NaeI 112 GCCGGC GCC^GGC 1885 GCCGGC NcoI 1986 CCATGG C^CATGG 3582 CCATGG NgoMIV 112 GCCGGC G^CCGGC 1885 GCCGGC PpuMI 2543 GGGACCT [GA]G^G[AT]CC[CT] 3480 AGGACCT PvuII 2122 CAGCTG CAG^CTG 2830 CAGCTG SspBI 1605 TGTACA T^GTACA 1869 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmaCI XmaI