################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI Alw44I ApaI ApaLI AscI Asp718I AspI AviII AvrII BbeI BbrPI BbvCI BciVI BglI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BssHII BssSI BstAPI BstEII BstPI BstUI CpoI EagI EclXI Eco52I EcoNI EcoO65I FseI FspI Hin1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NarI NdeI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflIII 213 ACATGT A^C[GA][CT]GT AhdI 2573 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 4021 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 2412 AGCGCT AGC^GCT AseI 1685 ATTAAT AT^TAAT AsnI 1685 ATTAAT AT^TAAT AspEI 2573 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1805 GTGCTC G[AT]GC[AT]^C AvaII 2212 GGACC G^G[AT]CC BamHI 400 GGATCC G^GATCC BanII 1432 GGGCTC G[GA]GC[CT]^C BsaAI 2639 TACGTG [CT]AC^GT[GA] BsaBI 1442 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 1805 GTGCTC G[AT]GC[AT]^C BspDI 44 ATCGAT AT^CGAT BspHI 3805 TCATGA T^CATGA BspLU11I 213 ACATGT A^CATGT BsrBI 3314 GAGCGG CCGCTC(-3/-3) BsrDI 2246 GCAATG GCAATG(2/0) BsrFI 2771 GCCGGC [GA]^CCGG[CT] BstBI 1331 TTCGAA TT^CGAA BstXI 2496 CCAATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 2771 GCCGGC [GA]^CCGG[CT] ClaI 44 ATCGAT AT^CGAT Eam1105I 2573 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2412 AGCGCT AGC^GCT EcoRV 461 GATATC GAT^ATC EcoT22I 3015 ATGCAT ATGCA^T HaeII 2412 AGCGCT [GA]GCGC^[CT] HgaI 4047 GCGTC GACGC(5/10) HindIII 3790 AAGCTT A^AGCTT MamI 1442 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2771 GCCGGC GCC^GGC NgoMIV 2771 GCCGGC G^CCGGC NheI 3501 GCTAGC G^CTAGC NsiI 3015 ATGCAT ATGCA^T NspV 1331 TTCGAA TT^CGAA PaeR7I 345 CTCGAG C^TCGAG PflMI 3237 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2211 AGGACCT [GA]G^G[AT]CC[CT] PshBI 1685 ATTAAT AT^TAAT PvuII 1867 CAGCTG CAG^CTG RcaI 3805 TCATGA T^CATGA SfuI 1331 TTCGAA TT^CGAA Van91I 3237 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 345 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 2649 GTATAC GT^[AC][GT]AC 4259 GTATAC AocI 1108 CCTGAGG CC^T[ACGT]AGG 3958 CCTGAGG Asp700I 852 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 944 GAAAGAATTC AvaI 345 CTCGAG C^[CT]CG[GA]G 3428 CCCGAG BalI 3938 TGGCCA TGG^CCA 4039 TGGCCA BcgI 1276 CGAAGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3376 CGAGGAGCATGC BclI 2002 TGATCA T^GATCA 3928 TGATCA BcnI 3055 CCGGG CC^[GC]GG 3346 CCGGG BlpI 1840 GCTTAGC GC^T[ACGT]AGC 2630 GCTAAGC Bpu1102I 1840 GCTTAGC GC^T[ACGT]AGC 2630 GCTAAGC BsoBI 345 CTCGAG C^[CT]CG[GA]G 3428 CCCGAG Bst1107I 2649 GTATAC GTA^TAC 4259 GTATAC BstZ17I 2649 GTATAC GTA^TAC 4259 GTATAC Bsu36I 1108 CCTGAGG CC^T[ACGT]AGG 3958 CCTGAGG CelII 1840 GCTTAGC GC^T[ACGT]AGC 2630 GCTAAGC DraII 2211 AGGACCT [GA]G^G[ACGT]CC[CT] 3463 GGGGCCT DrdI 381 GACAGTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2312 GACGGAGAGGTC Eco81I 1108 CCTGAGG CC^T[ACGT]AGG 3958 CCTGAGG EcoO109I 2211 AGGACCT [GA]G^G[ACGT]CC[CT] 3463 GGGGCCT FbaI 2002 TGATCA T^GATCA 3928 TGATCA HpaI 175 GTTAAC GTT^AAC 1375 GTTAAC MluNI 3938 TGGCCA TGG^CCA 4039 TGGCCA MscI 3938 TGGCCA TGG^CCA 4039 TGGCCA MspA1I 315 CAGCGG C[AC]G^C[GT]G 1867 CAGCTG NciI 3055 CCGGG CC^[GC]GG 3346 CCGGG NcoI 3178 CCATGG C^CATGG 4406 CCATGG PstI 2469 CTGCAG CTGCA^G 2748 CTGCAG SphI 1836 GCATGC GCATG^C 3382 GCATGC StuI 1860 AGGCCT AGG^CCT 4010 AGGCCT XbaI 3226 TCTAGA T^CTAGA 3848 TCTAGA XmnI 852 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 944 GAAAGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI ApoI BanI BbsI BbvI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraIII DsaI EaeI EarI EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII