##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I ApaI ApaLI
AscI Asp718I AspI AviII AvrII
BbeI BbrPI BbvCI BciVI BglI
BglII BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BssHII
BssSI BstAPI BstEII BstPI BstUI
CpoI EagI EclXI Eco52I EcoNI
EcoO65I FseI FspI Hin1I KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NarI NdeI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 213 ACATGT A^C[GA][CT]GT
AhdI 2573 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 4021 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2412 AGCGCT AGC^GCT
AseI 1685 ATTAAT AT^TAAT
AsnI 1685 ATTAAT AT^TAAT
AspEI 2573 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1805 GTGCTC G[AT]GC[AT]^C
AvaII 2212 GGACC G^G[AT]CC
BamHI 400 GGATCC G^GATCC
BanII 1432 GGGCTC G[GA]GC[CT]^C
BsaAI 2639 TACGTG [CT]AC^GT[GA]
BsaBI 1442 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1805 GTGCTC G[AT]GC[AT]^C
BspDI 44 ATCGAT AT^CGAT
BspHI 3805 TCATGA T^CATGA
BspLU11I 213 ACATGT A^CATGT
BsrBI 3314 GAGCGG CCGCTC(-3/-3)
BsrDI 2246 GCAATG GCAATG(2/0)
BsrFI 2771 GCCGGC [GA]^CCGG[CT]
BstBI 1331 TTCGAA TT^CGAA
BstXI 2496 CCAATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2771 GCCGGC [GA]^CCGG[CT]
ClaI 44 ATCGAT AT^CGAT
Eam1105I 2573 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2412 AGCGCT AGC^GCT
EcoRV 461 GATATC GAT^ATC
EcoT22I 3015 ATGCAT ATGCA^T
HaeII 2412 AGCGCT [GA]GCGC^[CT]
HgaI 4047 GCGTC GACGC(5/10)
HindIII 3790 AAGCTT A^AGCTT
MamI 1442 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2771 GCCGGC GCC^GGC
NgoMIV 2771 GCCGGC G^CCGGC
NheI 3501 GCTAGC G^CTAGC
NsiI 3015 ATGCAT ATGCA^T
NspV 1331 TTCGAA TT^CGAA
PaeR7I 345 CTCGAG C^TCGAG
PflMI 3237 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2211 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1685 ATTAAT AT^TAAT
PvuII 1867 CAGCTG CAG^CTG
RcaI 3805 TCATGA T^CATGA
SfuI 1331 TTCGAA TT^CGAA
Van91I 3237 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 345 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2649 GTATAC GT^[AC][GT]AC
4259 GTATAC
AocI 1108 CCTGAGG CC^T[ACGT]AGG
3958 CCTGAGG
Asp700I 852 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
944 GAAAGAATTC
AvaI 345 CTCGAG C^[CT]CG[GA]G
3428 CCCGAG
BalI 3938 TGGCCA TGG^CCA
4039 TGGCCA
BcgI 1276 CGAAGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3376 CGAGGAGCATGC
BclI 2002 TGATCA T^GATCA
3928 TGATCA
BcnI 3055 CCGGG CC^[GC]GG
3346 CCGGG
BlpI 1840 GCTTAGC GC^T[ACGT]AGC
2630 GCTAAGC
Bpu1102I 1840 GCTTAGC GC^T[ACGT]AGC
2630 GCTAAGC
BsoBI 345 CTCGAG C^[CT]CG[GA]G
3428 CCCGAG
Bst1107I 2649 GTATAC GTA^TAC
4259 GTATAC
BstZ17I 2649 GTATAC GTA^TAC
4259 GTATAC
Bsu36I 1108 CCTGAGG CC^T[ACGT]AGG
3958 CCTGAGG
CelII 1840 GCTTAGC GC^T[ACGT]AGC
2630 GCTAAGC
DraII 2211 AGGACCT [GA]G^G[ACGT]CC[CT]
3463 GGGGCCT
DrdI 381 GACAGTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2312 GACGGAGAGGTC
Eco81I 1108 CCTGAGG CC^T[ACGT]AGG
3958 CCTGAGG
EcoO109I 2211 AGGACCT [GA]G^G[ACGT]CC[CT]
3463 GGGGCCT
FbaI 2002 TGATCA T^GATCA
3928 TGATCA
HpaI 175 GTTAAC GTT^AAC
1375 GTTAAC
MluNI 3938 TGGCCA TGG^CCA
4039 TGGCCA
MscI 3938 TGGCCA TGG^CCA
4039 TGGCCA
MspA1I 315 CAGCGG C[AC]G^C[GT]G
1867 CAGCTG
NciI 3055 CCGGG CC^[GC]GG
3346 CCGGG
NcoI 3178 CCATGG C^CATGG
4406 CCATGG
PstI 2469 CTGCAG CTGCA^G
2748 CTGCAG
SphI 1836 GCATGC GCATG^C
3382 GCATGC
StuI 1860 AGGCCT AGG^CCT
4010 AGGCCT
XbaI 3226 TCTAGA T^CTAGA
3848 TCTAGA
XmnI 852 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
944 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI BanI BbsI BbvI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraIII
DsaI EaeI EarI EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII