################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AccIII AclI AflII AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BciVI BfrI BglII BlnI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 2479 GACGTC GACGT^C AgeI 2780 ACCGGT A^CCGGT AlwI 118 GATCC GGATC(4/5) AspI 216 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 34 GGCGCC GGCGC^C BbvCI 1607 GCTGAGG CCTCAGC(-5/-2) BcgI 1034 GCATCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2405 TGATCA T^GATCA BlpI 1516 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1516 GCTCAGC GC^T[ACGT]AGC BsiEI 339 CGGCCG CG[GA][CT]^CG BsmBI 3557 GAGACG CGTCTC(1/5) BsmI 3707 GCATTC GAATGC(1/-1) Bsp1407I 2763 TGTACA T^GTACA BsrGI 2763 TGTACA T^GTACA BssSI 2233 CACGAG CACGAG(-5/-1) BstYI 117 AGATCC [GA]^GATC[CT] CelII 1516 GCTCAGC GC^T[ACGT]AGC EagI 339 CGGCCG C^GGCCG EclXI 339 CGGCCG C^GGCCG Eco52I 339 CGGCCG C^GGCCG FbaI 2405 TGATCA T^GATCA HindIII 1409 AAGCTT A^AGCTT KasI 34 GGCGCC G^GCGCC MflI 117 AGATCC [GA]^GATC[CT] NarI 34 GGCGCC GG^CGCC NotI 338 GCGGCCGC GC^GGCCGC PflFI 216 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2780 ACCGGT A^CCGGT SapI 1831 GCTCTTC GCTCTTC(1/4) ScaI 174 AGTACT AGT^ACT SfoI 34 GGCGCC GGC^GCC SmaI 2681 CCCGGG CCC^GGG SspBI 2763 TGTACA T^GTACA SspI 3824 AATATT AAT^ATT TaqI 3429 TCGA T^CGA Tth111I 216 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 3429 TCGA T^CGA XhoII 117 AGATCC [GA]^GATC[CT] XmaCI 2681 CCCGGG C^CCGGG XmaI 2681 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 301 CGCG CG^CG 473 CGCG AcsI 3019 AAATTC [GA]^AATT[CT] 3948 AAATTT Alw44I 2722 GTGCAC G^TGCAC 2818 GTGCAC AlwNI 803 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 3004 CAGCTCCTG ApaLI 2722 GTGCAC G^TGCAC 2818 GTGCAC ApoI 3019 AAATTC [GA]^AATT[CT] 3948 AAATTT AvaI 1297 CTCGGG C^[CT]CG[GA]G 2681 CCCGGG BbrPI 521 CACGTG CAC^GTG 2287 CACGTG BsgI 293 CTGCAC GTGCAG(16/14) 326 GTGCAG BsoBI 1297 CTCGGG C^[CT]CG[GA]G 2681 CCCGGG BspLU11I 1313 ACATGT A^CATGT 2788 ACATGT BsrBI 3391 CCGCTC CCGCTC(-3/-3) 2440 GAGCGG BsrDI 2499 CATTGC GCAATG(2/0) 834 GCAATG BstUI 301 CGCG CG^CG 473 CGCG BstXI 1332 CCAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1581 CCAGCCACATGG EcoNI 796 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4121 CCTTCCAAAGG HaeII 34 GGCGCC [GA]GCGC^[CT] 1158 AGCGCC MvnI 301 CGCG CG^CG 473 CGCG NaeI 1380 GCCGGC GCC^GGC 2228 GCCGGC NgoMIV 1380 GCCGGC G^CCGGC 2228 GCCGGC NheI 3226 GCTAGC G^CTAGC 3931 GCTAGC PmaCI 521 CACGTG CAC^GTG 2287 CACGTG PmlI 521 CACGTG CAC^GTG 2287 CACGTG SmlI 617 CTCAAG C^T[CT][GA]AG 3554 CTTGAG SphI 2156 GCATGC GCATG^C 2657 GCATGC XcmI 1585 CCACATGGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2259 CCATGCGATGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AcyI AfaI AflIII AluI AocI ApaI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XmnI