##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BciVI
BfrI BglII BlnI BsaBI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2479 GACGTC GACGT^C
AgeI 2780 ACCGGT A^CCGGT
AlwI 118 GATCC GGATC(4/5)
AspI 216 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 34 GGCGCC GGCGC^C
BbvCI 1607 GCTGAGG CCTCAGC(-5/-2)
BcgI 1034 GCATCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2405 TGATCA T^GATCA
BlpI 1516 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1516 GCTCAGC GC^T[ACGT]AGC
BsiEI 339 CGGCCG CG[GA][CT]^CG
BsmBI 3557 GAGACG CGTCTC(1/5)
BsmI 3707 GCATTC GAATGC(1/-1)
Bsp1407I 2763 TGTACA T^GTACA
BsrGI 2763 TGTACA T^GTACA
BssSI 2233 CACGAG CACGAG(-5/-1)
BstYI 117 AGATCC [GA]^GATC[CT]
CelII 1516 GCTCAGC GC^T[ACGT]AGC
EagI 339 CGGCCG C^GGCCG
EclXI 339 CGGCCG C^GGCCG
Eco52I 339 CGGCCG C^GGCCG
FbaI 2405 TGATCA T^GATCA
HindIII 1409 AAGCTT A^AGCTT
KasI 34 GGCGCC G^GCGCC
MflI 117 AGATCC [GA]^GATC[CT]
NarI 34 GGCGCC GG^CGCC
NotI 338 GCGGCCGC GC^GGCCGC
PflFI 216 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2780 ACCGGT A^CCGGT
SapI 1831 GCTCTTC GCTCTTC(1/4)
ScaI 174 AGTACT AGT^ACT
SfoI 34 GGCGCC GGC^GCC
SmaI 2681 CCCGGG CCC^GGG
SspBI 2763 TGTACA T^GTACA
SspI 3824 AATATT AAT^ATT
TaqI 3429 TCGA T^CGA
Tth111I 216 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3429 TCGA T^CGA
XhoII 117 AGATCC [GA]^GATC[CT]
XmaCI 2681 CCCGGG C^CCGGG
XmaI 2681 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 301 CGCG CG^CG
473 CGCG
AcsI 3019 AAATTC [GA]^AATT[CT]
3948 AAATTT
Alw44I 2722 GTGCAC G^TGCAC
2818 GTGCAC
AlwNI 803 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3004 CAGCTCCTG
ApaLI 2722 GTGCAC G^TGCAC
2818 GTGCAC
ApoI 3019 AAATTC [GA]^AATT[CT]
3948 AAATTT
AvaI 1297 CTCGGG C^[CT]CG[GA]G
2681 CCCGGG
BbrPI 521 CACGTG CAC^GTG
2287 CACGTG
BsgI 293 CTGCAC GTGCAG(16/14)
326 GTGCAG
BsoBI 1297 CTCGGG C^[CT]CG[GA]G
2681 CCCGGG
BspLU11I 1313 ACATGT A^CATGT
2788 ACATGT
BsrBI 3391 CCGCTC CCGCTC(-3/-3)
2440 GAGCGG
BsrDI 2499 CATTGC GCAATG(2/0)
834 GCAATG
BstUI 301 CGCG CG^CG
473 CGCG
BstXI 1332 CCAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1581 CCAGCCACATGG
EcoNI 796 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4121 CCTTCCAAAGG
HaeII 34 GGCGCC [GA]GCGC^[CT]
1158 AGCGCC
MvnI 301 CGCG CG^CG
473 CGCG
NaeI 1380 GCCGGC GCC^GGC
2228 GCCGGC
NgoMIV 1380 GCCGGC G^CCGGC
2228 GCCGGC
NheI 3226 GCTAGC G^CTAGC
3931 GCTAGC
PmaCI 521 CACGTG CAC^GTG
2287 CACGTG
PmlI 521 CACGTG CAC^GTG
2287 CACGTG
SmlI 617 CTCAAG C^T[CT][GA]AG
3554 CTTGAG
SphI 2156 GCATGC GCATG^C
2657 GCATGC
XcmI 1585 CCACATGGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2259 CCATGCGATGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI AocI ApaI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XmnI