##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI Aor51HI ApaI AscI
Asp718I AspI AviII BbrPI BcgI
BciVI BglII BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BssHII BssSI BstEII BstPI
BstUI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NdeI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SexAI SfiI
SgrAI SmaI SnaBI SpeI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 801 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 801 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcnI 998 CCCGG CC^[GC]GG
BglI 1961 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4192 CACGTA [CT]AC^GT[GA]
BsmI 1844 GAATGC GAATGC(1/-1)
BsrBI 3219 GAGCGG CCGCTC(-3/-3)
BsrFI 1961 GCCGGC [GA]^CCGG[CT]
BstBI 4541 TTCGAA TT^CGAA
Cfr10I 1961 GCCGGC [GA]^CCGG[CT]
Eam1105I 801 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 907 GATATC GAT^ATC
HpaI 3803 GTTAAC GTT^AAC
NaeI 1961 GCCGGC GCC^GGC
NciI 998 CCCGG CC^[GC]GG
NgoMIV 1961 GCCGGC G^CCGGC
NheI 2577 GCTAGC G^CTAGC
NspV 4541 TTCGAA TT^CGAA
PflMI 2273 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 555 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2916 CCTGCAGG CCTGCA^GG
SfuI 4541 TTCGAA TT^CGAA
Sse8387I 2916 CCTGCAGG CCTGCA^GG
Van91I 2273 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 91 TCTAGA T^CTAGA
XcmI 1187 CCAGTAATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 595 GGCGCC G[GA]^CG[CT]C
1611 GGCGCC
AflII 475 CTTAAG C^TTAAG
3062 CTTAAG
Alw44I 3287 GTGCAC G^TGCAC
4245 GTGCAC
AocI 2828 CCTCAGG CC^T[ACGT]AGG
4055 CCTGAGG
ApaLI 3287 GTGCAC G^TGCAC
4245 GTGCAC
AseI 3636 ATTAAT AT^TAAT
3756 ATTAAT
AsnI 3636 ATTAAT AT^TAAT
3756 ATTAAT
Asp700I 975 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2949 GAAACTCTTC
BamHI 789 GGATCC G^GATCC
3561 GGATCC
BbeI 595 GGCGCC GGCGC^C
1611 GGCGCC
BclI 1284 TGATCA T^GATCA
3137 TGATCA
BfrI 475 CTTAAG C^TTAAG
3062 CTTAAG
BlpI 343 GCTCAGC GC^T[ACGT]AGC
3097 GCTCAGC
Bpu1102I 343 GCTCAGC GC^T[ACGT]AGC
3097 GCTCAGC
BsaHI 595 GGCGCC G[GA]^CG[CT]C
1611 GGCGCC
BsrDI 268 GCAATG GCAATG(2/0)
1927 GCAATG
Bst1107I 884 GTATAC GTA^TAC
3939 GTATAC
BstZ17I 884 GTATAC GTA^TAC
3939 GTATAC
Bsu36I 2828 CCTCAGG CC^T[ACGT]AGG
4055 CCTGAGG
CelII 343 GCTCAGC GC^T[ACGT]AGC
3097 GCTCAGC
EarI 2109 CTCTTC CTCTTC(1/4)
2953 CTCTTC
Eco81I 2828 CCTCAGG CC^T[ACGT]AGG
4055 CCTGAGG
FbaI 1284 TGATCA T^GATCA
3137 TGATCA
HaeII 595 GGCGCC [GA]GCGC^[CT]
1611 GGCGCC
HapII 999 CCGG C^CGG
1962 CCGG
HgaI 480 GACGC GACGC(5/10)
2002 GACGC
Hin1I 595 GGCGCC G[GA]^CG[CT]C
1611 GGCGCC
HincII 649 GTTGAC GT[CT]^[GA]AC
3803 GTTAAC
HindII 649 GTTGAC GT[CT]^[GA]AC
3803 GTTAAC
HpaII 999 CCGG C^CGG
1962 CCGG
KasI 595 GGCGCC G^GCGCC
1611 GGCGCC
Ksp632I 2109 CTCTTC CTCTTC(1/4)
2953 CTCTTC
MspI 999 CCGG C^CGG
1962 CCGG
NarI 595 GGCGCC GG^CGCC
1611 GGCGCC
PshBI 3636 ATTAAT AT^TAAT
3756 ATTAAT
SacI 391 GAGCTC GAGCT^C
2072 GAGCTC
SfoI 595 GGCGCC GGC^GCC
1611 GGCGCC
SmlI 475 CTTAAG C^T[CT][GA]AG
3062 CTTAAG
SphI 2812 GCATGC GCATG^C
3278 GCATGC
XmnI 975 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2949 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII