################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Aor51HI AscI Asp700I Asp718I AspEI AspI AviII BbeI BbrPI BciVI BfrI BglII BsaAI BsaBI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI HindIII KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SapI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1333 GACGTC GACGT^C AccI 3370 GTAGAC GT^[AC][GT]AC AccII 59 CGCG CG^CG AcyI 1333 GACGTC G[GA]^CG[CT]C AflIII 789 ACATGT A^C[GA][CT]GT Alw44I 4211 GTGCAC G^TGCAC ApaLI 4211 GTGCAC G^TGCAC AseI 3662 ATTAAT AT^TAAT AsnI 3662 ATTAAT AT^TAAT AvrII 1871 CCTAGG C^CTAGG BanI 4443 GGCACC G^G[CT][GA]CC BcgI 335 CGAGAGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 208 TGATCA T^GATCA BlnI 1871 CCTAGG C^CTAGG BsaHI 1333 GACGTC G[GA]^CG[CT]C BsaWI 3595 ACCGGA [AT]^CCGG[AT] BsmBI 1394 CGTCTC CGTCTC(1/5) BsmI 3423 GCATTC GAATGC(1/-1) BspHI 211 TCATGA T^CATGA BspLU11I 789 ACATGT A^CATGT BsrDI 2355 GCAATG GCAATG(2/0) BstAPI 1819 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 59 CGCG CG^CG BstXI 424 CCACACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG FbaI 208 TGATCA T^GATCA Hin1I 1333 GACGTC G[GA]^CG[CT]C HpaI 4615 GTTAAC GTT^AAC KspI 58 CCGCGG CCGC^GG MvnI 59 CGCG CG^CG NaeI 2608 GCCGGC GCC^GGC NcoI 1092 CCATGG C^CATGG NgoMIV 2608 GCCGGC G^CCGGC PaeR7I 3467 CTCGAG C^TCGAG PshBI 3662 ATTAAT AT^TAAT RcaI 211 TCATGA T^CATGA SacI 497 GAGCTC GAGCT^C SacII 58 CCGCGG CCGC^GG SbfI 1577 CCTGCAGG CCTGCA^GG ScaI 2890 AGTACT AGT^ACT SexAI 880 ACCTGGT A^CC[AT]GGT SfiI 636 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3175 GCATGC GCATG^C Sse8387I 1577 CCTGCAGG CCTGCA^GG StuI 3392 AGGCCT AGG^CCT XbaI 1864 TCTAGA T^CTAGA XhoI 3467 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 347 AAATTT [GA]^AATT[CT] 4556 GAATTT ApoI 347 AAATTT [GA]^AATT[CT] 4556 GAATTT BalI 3494 TGGCCA TGG^CCA 4309 TGGCCA BbvCI 1441 CCTCAGC CCTCAGC(-5/-2) 539 GCTGAGG BlpI 2333 GCTCAGC GC^T[ACGT]AGC 2367 GCTCAGC Bpu1102I 2333 GCTCAGC GC^T[ACGT]AGC 2367 GCTCAGC Bsp1407I 792 TGTACA T^GTACA 3283 TGTACA BsrGI 792 TGTACA T^GTACA 3283 TGTACA CelII 2333 GCTCAGC GC^T[ACGT]AGC 2367 GCTCAGC CfoI 537 GCGC GCG^C 3865 GCGC EarI 485 GAAGAG CTCTTC(1/4) 1616 GAAGAG HaeII 536 GGCGCT [GA]GCGC^[CT] 3864 AGCGCC HgaI 4048 GACGC GACGC(5/10) 1918 GCGTC HhaI 537 GCGC GCG^C 3865 GCGC HinP1I 537 GCGC G^CGC 3865 GCGC Ksp632I 485 GAAGAG CTCTTC(1/4) 1616 GAAGAG MluNI 3494 TGGCCA TGG^CCA 4309 TGGCCA MscI 3494 TGGCCA TGG^CCA 4309 TGGCCA MslI 1057 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1216 CATGGAAATG NheI 847 GCTAGC G^CTAGC 3553 GCTAGC PvuII 1709 CAGCTG CAG^CTG 3709 CAGCTG SspBI 792 TGTACA T^GTACA 3283 TGTACA XcmI 1348 CCAGGCTTCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4216 CCAAGATCAAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BamHI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII