##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI Asp700I Asp718I
AspEI AspI AviII BbeI BbrPI
BciVI BfrI BglII BsaAI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI FspI
HindIII KasI KpnI MamI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SapI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tth111I XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1333 GACGTC GACGT^C
AccI 3370 GTAGAC GT^[AC][GT]AC
AccII 59 CGCG CG^CG
AcyI 1333 GACGTC G[GA]^CG[CT]C
AflIII 789 ACATGT A^C[GA][CT]GT
Alw44I 4211 GTGCAC G^TGCAC
ApaLI 4211 GTGCAC G^TGCAC
AseI 3662 ATTAAT AT^TAAT
AsnI 3662 ATTAAT AT^TAAT
AvrII 1871 CCTAGG C^CTAGG
BanI 4443 GGCACC G^G[CT][GA]CC
BcgI 335 CGAGAGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 208 TGATCA T^GATCA
BlnI 1871 CCTAGG C^CTAGG
BsaHI 1333 GACGTC G[GA]^CG[CT]C
BsaWI 3595 ACCGGA [AT]^CCGG[AT]
BsmBI 1394 CGTCTC CGTCTC(1/5)
BsmI 3423 GCATTC GAATGC(1/-1)
BspHI 211 TCATGA T^CATGA
BspLU11I 789 ACATGT A^CATGT
BsrDI 2355 GCAATG GCAATG(2/0)
BstAPI 1819 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 59 CGCG CG^CG
BstXI 424 CCACACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 208 TGATCA T^GATCA
Hin1I 1333 GACGTC G[GA]^CG[CT]C
HpaI 4615 GTTAAC GTT^AAC
KspI 58 CCGCGG CCGC^GG
MvnI 59 CGCG CG^CG
NaeI 2608 GCCGGC GCC^GGC
NcoI 1092 CCATGG C^CATGG
NgoMIV 2608 GCCGGC G^CCGGC
PaeR7I 3467 CTCGAG C^TCGAG
PshBI 3662 ATTAAT AT^TAAT
RcaI 211 TCATGA T^CATGA
SacI 497 GAGCTC GAGCT^C
SacII 58 CCGCGG CCGC^GG
SbfI 1577 CCTGCAGG CCTGCA^GG
ScaI 2890 AGTACT AGT^ACT
SexAI 880 ACCTGGT A^CC[AT]GGT
SfiI 636 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3175 GCATGC GCATG^C
Sse8387I 1577 CCTGCAGG CCTGCA^GG
StuI 3392 AGGCCT AGG^CCT
XbaI 1864 TCTAGA T^CTAGA
XhoI 3467 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 347 AAATTT [GA]^AATT[CT]
4556 GAATTT
ApoI 347 AAATTT [GA]^AATT[CT]
4556 GAATTT
BalI 3494 TGGCCA TGG^CCA
4309 TGGCCA
BbvCI 1441 CCTCAGC CCTCAGC(-5/-2)
539 GCTGAGG
BlpI 2333 GCTCAGC GC^T[ACGT]AGC
2367 GCTCAGC
Bpu1102I 2333 GCTCAGC GC^T[ACGT]AGC
2367 GCTCAGC
Bsp1407I 792 TGTACA T^GTACA
3283 TGTACA
BsrGI 792 TGTACA T^GTACA
3283 TGTACA
CelII 2333 GCTCAGC GC^T[ACGT]AGC
2367 GCTCAGC
CfoI 537 GCGC GCG^C
3865 GCGC
EarI 485 GAAGAG CTCTTC(1/4)
1616 GAAGAG
HaeII 536 GGCGCT [GA]GCGC^[CT]
3864 AGCGCC
HgaI 4048 GACGC GACGC(5/10)
1918 GCGTC
HhaI 537 GCGC GCG^C
3865 GCGC
HinP1I 537 GCGC G^CGC
3865 GCGC
Ksp632I 485 GAAGAG CTCTTC(1/4)
1616 GAAGAG
MluNI 3494 TGGCCA TGG^CCA
4309 TGGCCA
MscI 3494 TGGCCA TGG^CCA
4309 TGGCCA
MslI 1057 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1216 CATGGAAATG
NheI 847 GCTAGC G^CTAGC
3553 GCTAGC
PvuII 1709 CAGCTG CAG^CTG
3709 CAGCTG
SspBI 792 TGTACA T^GTACA
3283 TGTACA
XcmI 1348 CCAGGCTTCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4216 CCAAGATCAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BamHI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII