################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AclI AhdI Alw44I ApaI ApaLI AscI AspEI AvrII BbeI BbrPI BcgI BglI BlnI BsiWI BsmBI BssHII BssSI Bst1107I BstZ17I CpoI EagI Eam1105I EclXI Eco52I FseI KasI KspI NaeI NarI NgoMIV NotI NruI PacI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 3331 GACGTC GACGT^C Acc65I 4849 GGTACC G^GTACC AccI 1338 GTCTAC GT^[AC][GT]AC AccIII 3240 TCCGGA T^CCGGA AflII 2009 CTTAAG C^TTAAG AgeI 5142 ACCGGT A^CCGGT AocI 2789 CCTGAGG CC^T[ACGT]AGG Asp718I 4849 GGTACC G^GTACC AspI 2974 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3224 TGCGCA TGC^GCA BciVI 4399 GTATCC GTATCC(6/5) BclI 850 TGATCA T^GATCA BfrI 2009 CTTAAG C^TTAAG BglII 2594 AGATCT A^GATCT BsaI 4103 GAGACC GGTCTC(1/5) BseAI 3240 TCCGGA T^CCGGA BsiEI 4584 CGGTCG CG[GA][CT]^CG BspDI 471 ATCGAT AT^CGAT BspEI 3240 TCCGGA T^CCGGA BspLU11I 4483 ACATGT A^CATGT BstBI 2443 TTCGAA TT^CGAA Bsu36I 2789 CCTGAGG CC^T[ACGT]AGG ClaI 471 ATCGAT AT^CGAT DraIII 2378 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2789 CCTGAGG CC^T[ACGT]AGG EcoNI 4295 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2198 GAATTC G^AATTC EcoRV 4813 GATATC GAT^ATC FbaI 850 TGATCA T^GATCA FspI 3224 TGCGCA TGC^GCA HindIII 2050 AAGCTT A^AGCTT KpnI 4849 GGTACC GGTAC^C MluI 3255 ACGCGT A^CGCGT MroI 3240 TCCGGA T^CCGGA NcoI 2358 CCATGG C^CATGG NdeI 2347 CATATG CA^TATG NheI 1952 GCTAGC G^CTAGC NspV 2443 TTCGAA TT^CGAA PaeR7I 241 CTCGAG C^TCGAG PflFI 2974 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 5142 ACCGGT A^CCGGT ScaI 1278 AGTACT AGT^ACT SfuI 2443 TTCGAA TT^CGAA SmaI 4641 CCCGGG CCC^GGG SnaBI 4686 TACGTA TAC^GTA SphI 1841 GCATGC GCATG^C Tth111I 2974 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 974 TCTAGA T^CTAGA XcmI 1008 CCACCAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 241 CTCGAG C^TCGAG XmaCI 4641 CCCGGG C^CCGGG XmaI 4641 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Aor51HI 3270 AGCGCT AGC^GCT 3548 AGCGCT AseI 2628 ATTAAT AT^TAAT 4056 ATTAAT AsnI 2628 ATTAAT AT^TAAT 4056 ATTAAT Asp700I 525 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1227 GAAGAGCTTC BamHI 87 GGATCC G^GATCC 4513 GGATCC BbsI 3236 GTCTTC GAAGAC(2/6) 4987 GTCTTC BpuAI 3236 GTCTTC GAAGAC(2/6) 4987 GTCTTC BsaWI 3240 TCCGGA [AT]^CCGG[AT] 5142 ACCGGT BseRI 337 CTCCTC GAGGAG(10/8) 1545 GAGGAG Bsp1407I 1287 TGTACA T^GTACA 5051 TGTACA BspHI 2620 TCATGA T^CATGA 3956 TCATGA BspMI 1150 ACCTGC ACCTGC(4/8) 3907 ACCTGC BsrBI 3061 CCGCTC CCGCTC(-3/-3) 1899 GAGCGG BsrGI 1287 TGTACA T^GTACA 5051 TGTACA BstEII 365 GGTAACC G^GT[ACGT]ACC 3358 GGTTACC BstPI 365 GGTAACC G^GT[ACGT]ACC 3358 GGTTACC BstXI 1011 CCAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4974 CCACATCCTTGG DraI 129 TTTAAA TTT^AAA 5317 TTTAAA DraII 1793 AGGCCCT [GA]G^G[ACGT]CC[CT] 2726 AGGCCCT DrdI 1329 GACATCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3325 GACGATGACGTC DsaI 2358 CCATGG C^C[GA][CT]GG 2521 CCGTGG Eco47III 3270 AGCGCT AGC^GCT 3548 AGCGCT EcoO109I 1793 AGGCCCT [GA]G^G[ACGT]CC[CT] 2726 AGGCCCT EcoO65I 365 GGTAACC G^GT[ACGT]ACC 3358 GGTTACC FauI 3115 GCGGG CCCGC(4/6) 3423 GCGGG HaeII 3270 AGCGCT [GA]GCGC^[CT] 3548 AGCGCT HincII 354 GTTAAC GT[CT]^[GA]AC 2457 GTTAAC HindII 354 GTTAAC GT[CT]^[GA]AC 2457 GTTAAC HpaI 354 GTTAAC GTT^AAC 2457 GTTAAC PshBI 2628 ATTAAT AT^TAAT 4056 ATTAAT RcaI 2620 TCATGA T^CATGA 3956 TCATGA SacI 1134 GAGCTC GAGCT^C 4023 GAGCTC SapI 594 GAAGAGC GCTCTTC(1/4) 1227 GAAGAGC SspBI 1287 TGTACA T^GTACA 5051 TGTACA SspI 528 AATATT AAT^ATT 4243 AATATT StuI 1352 AGGCCT AGG^CCT 2786 AGGCCT XmnI 525 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1227 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII