##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AclI AhdI Alw44I ApaI ApaLI
AscI AspEI AvrII BbeI BbrPI
BcgI BglI BlnI BsiWI BsmBI
BssHII BssSI Bst1107I BstZ17I CpoI
EagI Eam1105I EclXI Eco52I FseI
KasI KspI NaeI NarI NgoMIV
NotI NruI PacI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 3331 GACGTC GACGT^C
Acc65I 4849 GGTACC G^GTACC
AccI 1338 GTCTAC GT^[AC][GT]AC
AccIII 3240 TCCGGA T^CCGGA
AflII 2009 CTTAAG C^TTAAG
AgeI 5142 ACCGGT A^CCGGT
AocI 2789 CCTGAGG CC^T[ACGT]AGG
Asp718I 4849 GGTACC G^GTACC
AspI 2974 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3224 TGCGCA TGC^GCA
BciVI 4399 GTATCC GTATCC(6/5)
BclI 850 TGATCA T^GATCA
BfrI 2009 CTTAAG C^TTAAG
BglII 2594 AGATCT A^GATCT
BsaI 4103 GAGACC GGTCTC(1/5)
BseAI 3240 TCCGGA T^CCGGA
BsiEI 4584 CGGTCG CG[GA][CT]^CG
BspDI 471 ATCGAT AT^CGAT
BspEI 3240 TCCGGA T^CCGGA
BspLU11I 4483 ACATGT A^CATGT
BstBI 2443 TTCGAA TT^CGAA
Bsu36I 2789 CCTGAGG CC^T[ACGT]AGG
ClaI 471 ATCGAT AT^CGAT
DraIII 2378 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2789 CCTGAGG CC^T[ACGT]AGG
EcoNI 4295 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2198 GAATTC G^AATTC
EcoRV 4813 GATATC GAT^ATC
FbaI 850 TGATCA T^GATCA
FspI 3224 TGCGCA TGC^GCA
HindIII 2050 AAGCTT A^AGCTT
KpnI 4849 GGTACC GGTAC^C
MluI 3255 ACGCGT A^CGCGT
MroI 3240 TCCGGA T^CCGGA
NcoI 2358 CCATGG C^CATGG
NdeI 2347 CATATG CA^TATG
NheI 1952 GCTAGC G^CTAGC
NspV 2443 TTCGAA TT^CGAA
PaeR7I 241 CTCGAG C^TCGAG
PflFI 2974 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5142 ACCGGT A^CCGGT
ScaI 1278 AGTACT AGT^ACT
SfuI 2443 TTCGAA TT^CGAA
SmaI 4641 CCCGGG CCC^GGG
SnaBI 4686 TACGTA TAC^GTA
SphI 1841 GCATGC GCATG^C
Tth111I 2974 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 974 TCTAGA T^CTAGA
XcmI 1008 CCACCAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 241 CTCGAG C^TCGAG
XmaCI 4641 CCCGGG C^CCGGG
XmaI 4641 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Aor51HI 3270 AGCGCT AGC^GCT
3548 AGCGCT
AseI 2628 ATTAAT AT^TAAT
4056 ATTAAT
AsnI 2628 ATTAAT AT^TAAT
4056 ATTAAT
Asp700I 525 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1227 GAAGAGCTTC
BamHI 87 GGATCC G^GATCC
4513 GGATCC
BbsI 3236 GTCTTC GAAGAC(2/6)
4987 GTCTTC
BpuAI 3236 GTCTTC GAAGAC(2/6)
4987 GTCTTC
BsaWI 3240 TCCGGA [AT]^CCGG[AT]
5142 ACCGGT
BseRI 337 CTCCTC GAGGAG(10/8)
1545 GAGGAG
Bsp1407I 1287 TGTACA T^GTACA
5051 TGTACA
BspHI 2620 TCATGA T^CATGA
3956 TCATGA
BspMI 1150 ACCTGC ACCTGC(4/8)
3907 ACCTGC
BsrBI 3061 CCGCTC CCGCTC(-3/-3)
1899 GAGCGG
BsrGI 1287 TGTACA T^GTACA
5051 TGTACA
BstEII 365 GGTAACC G^GT[ACGT]ACC
3358 GGTTACC
BstPI 365 GGTAACC G^GT[ACGT]ACC
3358 GGTTACC
BstXI 1011 CCAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4974 CCACATCCTTGG
DraI 129 TTTAAA TTT^AAA
5317 TTTAAA
DraII 1793 AGGCCCT [GA]G^G[ACGT]CC[CT]
2726 AGGCCCT
DrdI 1329 GACATCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3325 GACGATGACGTC
DsaI 2358 CCATGG C^C[GA][CT]GG
2521 CCGTGG
Eco47III 3270 AGCGCT AGC^GCT
3548 AGCGCT
EcoO109I 1793 AGGCCCT [GA]G^G[ACGT]CC[CT]
2726 AGGCCCT
EcoO65I 365 GGTAACC G^GT[ACGT]ACC
3358 GGTTACC
FauI 3115 GCGGG CCCGC(4/6)
3423 GCGGG
HaeII 3270 AGCGCT [GA]GCGC^[CT]
3548 AGCGCT
HincII 354 GTTAAC GT[CT]^[GA]AC
2457 GTTAAC
HindII 354 GTTAAC GT[CT]^[GA]AC
2457 GTTAAC
HpaI 354 GTTAAC GTT^AAC
2457 GTTAAC
PshBI 2628 ATTAAT AT^TAAT
4056 ATTAAT
RcaI 2620 TCATGA T^CATGA
3956 TCATGA
SacI 1134 GAGCTC GAGCT^C
4023 GAGCTC
SapI 594 GAAGAGC GCTCTTC(1/4)
1227 GAAGAGC
SspBI 1287 TGTACA T^GTACA
5051 TGTACA
SspI 528 AATATT AAT^ATT
4243 AATATT
StuI 1352 AGGCCT AGG^CCT
2786 AGGCCT
XmnI 525 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1227 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII