################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AviII BamHI BbrPI BclI BfrI BseAI BsiWI BspDI BspEI BspHI BspLU11I BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI Eco47III EcoO65I EcoRI EcoRV FbaI FseI FspI HpaI KpnI KspI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SgrAI SmaI SnaBI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflIII 1291 ACGTGT A^C[GA][CT]GT AgeI 1233 ACCGGT A^CCGGT AhdI 2788 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3384 CCTCAGG CC^T[ACGT]AGG Asp700I 1875 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2788 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2067 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1409 CCCGAG C^[CT]CG[GA]G AvrII 4288 CCTAGG C^CTAGG BcgI 2517 GCAGTCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1481 GTATCC GTATCC(6/5) BglI 2698 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1018 AGATCT A^GATCT BlnI 4288 CCTAGG C^CTAGG BsaAI 1290 TACGTG [CT]AC^GT[GA] BsaBI 3086 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 3365 CTGCAC GTGCAG(16/14) BsiEI 588 CGGCCG CG[GA][CT]^CG BsmBI 598 GAGACG CGTCTC(1/5) BsmI 2897 GCATTC GAATGC(1/-1) BsoBI 1409 CCCGAG C^[CT]CG[GA]G BsrBI 2376 CCGCTC CCGCTC(-3/-3) BsrFI 1233 ACCGGT [GA]^CCGG[CT] BssHII 39 GCGCGC G^CGCGC BstAPI 3933 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2165 TTCGAA TT^CGAA BstYI 1018 AGATCT [GA]^GATC[CT] Bsu36I 3384 CCTCAGG CC^T[ACGT]AGG Cfr10I 1233 ACCGGT [GA]^CCGG[CT] DraIII 2803 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3867 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1745 CCATGG C^C[GA][CT]GG EagI 588 CGGCCG C^GGCCG Eam1105I 2788 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 588 CGGCCG C^GGCCG Eco52I 588 CGGCCG C^GGCCG Eco81I 3384 CCTCAGG CC^T[ACGT]AGG EcoT22I 876 ATGCAT ATGCA^T HincII 2904 GTCAAC GT[CT]^[GA]AC HindII 2904 GTCAAC GT[CT]^[GA]AC MamI 3086 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1018 AGATCT [GA]^GATC[CT] NcoI 1745 CCATGG C^CATGG NsiI 876 ATGCAT ATGCA^T NspV 2165 TTCGAA TT^CGAA PflFI 2067 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2802 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1233 ACCGGT A^CCGGT PshAI 3041 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfuI 2165 TTCGAA TT^CGAA SpeI 2935 ACTAGT A^CTAGT SphI 874 GCATGC GCATG^C SspI 4238 AATATT AAT^ATT StuI 4285 AGGCCT AGG^CCT Tth111I 2067 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2802 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1018 AGATCT [GA]^GATC[CT] XmnI 1875 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 1179 GAGCTC G[AT]GC[AT]^C 1448 GAGCTC BbeI 123 GGCGCC GGCGC^C 3342 GGCGCC BbvCI 2145 CCTCAGC CCTCAGC(-5/-2) 2175 CCTCAGC BlpI 3230 GCTCAGC GC^T[ACGT]AGC 4688 GCTAAGC Bpu1102I 3230 GCTCAGC GC^T[ACGT]AGC 4688 GCTAAGC BsaWI 1233 ACCGGT [AT]^CCGG[AT] 2408 TCCGGT BsiHKAI 1179 GAGCTC G[AT]GC[AT]^C 1448 GAGCTC BstXI 1382 CCACCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1937 CCAGGTATTTGG CelII 3230 GCTCAGC GC^T[ACGT]AGC 4688 GCTAAGC HgaI 1829 GACGC GACGC(5/10) 2990 GACGC HindIII 756 AAGCTT A^AGCTT 2430 AAGCTT KasI 123 GGCGCC G^GCGCC 3342 GGCGCC NarI 123 GGCGCC GG^CGCC 3342 GGCGCC PpuMI 3046 GGGTCCC [GA]G^G[AT]CC[CT] 3505 GGGACCC PvuII 476 CAGCTG CAG^CTG 2996 CAGCTG SacI 1179 GAGCTC GAGCT^C 1448 GAGCTC SexAI 2253 ACCAGGT A^CC[AT]GGT 3694 ACCAGGT SfoI 123 GGCGCC GGC^GCC 3342 GGCGCC XcmI 1250 CCATATTTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1367 CCAACTTTAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I