##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AviII
BamHI BbrPI BclI BfrI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI Eco47III EcoO65I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI KspI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SmaI SnaBI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AflIII 1291 ACGTGT A^C[GA][CT]GT
AgeI 1233 ACCGGT A^CCGGT
AhdI 2788 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3384 CCTCAGG CC^T[ACGT]AGG
Asp700I 1875 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2788 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2067 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1409 CCCGAG C^[CT]CG[GA]G
AvrII 4288 CCTAGG C^CTAGG
BcgI 2517 GCAGTCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1481 GTATCC GTATCC(6/5)
BglI 2698 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1018 AGATCT A^GATCT
BlnI 4288 CCTAGG C^CTAGG
BsaAI 1290 TACGTG [CT]AC^GT[GA]
BsaBI 3086 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3365 CTGCAC GTGCAG(16/14)
BsiEI 588 CGGCCG CG[GA][CT]^CG
BsmBI 598 GAGACG CGTCTC(1/5)
BsmI 2897 GCATTC GAATGC(1/-1)
BsoBI 1409 CCCGAG C^[CT]CG[GA]G
BsrBI 2376 CCGCTC CCGCTC(-3/-3)
BsrFI 1233 ACCGGT [GA]^CCGG[CT]
BssHII 39 GCGCGC G^CGCGC
BstAPI 3933 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2165 TTCGAA TT^CGAA
BstYI 1018 AGATCT [GA]^GATC[CT]
Bsu36I 3384 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1233 ACCGGT [GA]^CCGG[CT]
DraIII 2803 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3867 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1745 CCATGG C^C[GA][CT]GG
EagI 588 CGGCCG C^GGCCG
Eam1105I 2788 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 588 CGGCCG C^GGCCG
Eco52I 588 CGGCCG C^GGCCG
Eco81I 3384 CCTCAGG CC^T[ACGT]AGG
EcoT22I 876 ATGCAT ATGCA^T
HincII 2904 GTCAAC GT[CT]^[GA]AC
HindII 2904 GTCAAC GT[CT]^[GA]AC
MamI 3086 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1018 AGATCT [GA]^GATC[CT]
NcoI 1745 CCATGG C^CATGG
NsiI 876 ATGCAT ATGCA^T
NspV 2165 TTCGAA TT^CGAA
PflFI 2067 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2802 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1233 ACCGGT A^CCGGT
PshAI 3041 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 2165 TTCGAA TT^CGAA
SpeI 2935 ACTAGT A^CTAGT
SphI 874 GCATGC GCATG^C
SspI 4238 AATATT AAT^ATT
StuI 4285 AGGCCT AGG^CCT
Tth111I 2067 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2802 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1018 AGATCT [GA]^GATC[CT]
XmnI 1875 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 1179 GAGCTC G[AT]GC[AT]^C
1448 GAGCTC
BbeI 123 GGCGCC GGCGC^C
3342 GGCGCC
BbvCI 2145 CCTCAGC CCTCAGC(-5/-2)
2175 CCTCAGC
BlpI 3230 GCTCAGC GC^T[ACGT]AGC
4688 GCTAAGC
Bpu1102I 3230 GCTCAGC GC^T[ACGT]AGC
4688 GCTAAGC
BsaWI 1233 ACCGGT [AT]^CCGG[AT]
2408 TCCGGT
BsiHKAI 1179 GAGCTC G[AT]GC[AT]^C
1448 GAGCTC
BstXI 1382 CCACCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1937 CCAGGTATTTGG
CelII 3230 GCTCAGC GC^T[ACGT]AGC
4688 GCTAAGC
HgaI 1829 GACGC GACGC(5/10)
2990 GACGC
HindIII 756 AAGCTT A^AGCTT
2430 AAGCTT
KasI 123 GGCGCC G^GCGCC
3342 GGCGCC
NarI 123 GGCGCC GG^CGCC
3342 GGCGCC
PpuMI 3046 GGGTCCC [GA]G^G[AT]CC[CT]
3505 GGGACCC
PvuII 476 CAGCTG CAG^CTG
2996 CAGCTG
SacI 1179 GAGCTC GAGCT^C
1448 GAGCTC
SexAI 2253 ACCAGGT A^CC[AT]GGT
3694 ACCAGGT
SfoI 123 GGCGCC GGC^GCC
3342 GGCGCC
XcmI 1250 CCATATTTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1367 CCAACTTTAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I